Relationships between plasmids and phenotypes of presumptive strains of Vibrio anguillarum isolated from different fish species

Wiik, Ragnhild; Hoff, Kjell Arne; Andersen, Knut-Jan; Daae, Frida Lise

doi:10.1128/aem.55.4.826-831.1989

Cited by 38 publications

(23 citation statements)

References 31 publications

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“…The 65to 70-kilobase plasmid, noted to be the only plasmid in 62% of the V. anguillarum strains we isolated, corresponds in size to the pJM1 plasmid, first noted by Crosa et al (3,4) in highly virulent strains of V. anguillarum and later described in detail with respect to plasmid-encoded iron-sequestering and iron-binding proteins (15). The 65to 70-kilobase plasmids in Danish isolates of V. anguillarum have HindIll profiles similar to that previously published for pJM1 by Wiik et al (16), and we assume that they belong to the same class of virulence plasmids.…”

Section: Discussionsupporting

confidence: 85%

“…on September 1, 2020 by guest http://aem.asm.org/ Downloaded from II 41 53 III 20 26 IV 1 1 V 2 3 VI 1 1 patterns produced thereby were similar to the Hindlll pattern previously described for the virulence plasmid pJM1 (16).…”

Section: Resultssupporting

confidence: 79%

“…Plasmid profiling is of limited value; e.g., Danish isolates have been shown to have only two different plasmid profiles, and 62% of the strains carry only one 65to 70-kilobase plasmid (unpublished data). Discrimination based on biotyping has been used for V. anguillarum (16), but most Danish isolates of serovar 01 fall within the same biogroup (unpublished data), so this method is also of limited value.…”

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confidence: 99%

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Restriction fragment length polymorphism of the Vibrio anguillarum serovar O1 virulence plasmid

Olsen¹,

Larsen²

1990

Appl Environ Microbiol

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Seventy-eight strains of Vibrio anguillarum serovar 01, all harboring one 65-to 70-kilobase plasmid, were typed according to restriction fragment length polymorphism of the plasmid. Six types, three of which comprised 96% of the strains examined, were produced with the restriction endonuclease BamHI. The fragment length polymorphism type did not correlate to any of 12 different phenotypic properties tested.

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Section: Discussionsupporting

confidence: 85%

Section: Resultssupporting

confidence: 79%

mentioning

confidence: 99%

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Restriction fragment length polymorphism of the Vibrio anguillarum serovar O1 virulence plasmid

Olsen¹,

Larsen²

1990

Appl Environ Microbiol

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“…It has been stressed that some host preferences exist within the 'pathogenic serovars' and that they interact with the host in different ways (Wiik et al 1989). These may be conferred by and O^ strains have shown the saniie level of virulence (Toranzo et al 1987).…”

Section: Resultsmentioning

confidence: 99%

Vibrio anguillarum serovars associated with vibriosis in fish

Larsen¹,

Pedersen²,

Dalsgaard³

1994

Journal of Fish Diseases

116

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A total ol" 317 Vibrio anguillantm strains isolated from diseased fish together with 14 V. anguillarum serogroup O2 and V. ordalii type strains were serotyped using the European scrotyping system. Marked speeies difterenees were reeorded. In isolates from belonged to other serogroups or were non-typeablc. Figures for turbot were similar to those from salmonids. In 32 isolates from sea bass, sea bream and mullet, most strains belonged to serogroup Ol. while one was O2a. one O7, and the rest non-typcable In cod sero\ar O2 was dominant while only a minor proportion belonged to other serogroups or were non-typeable. The eel isolates belonged equally to serovars O2 and O3. All O2 strains were subtyped with absorbed O2a and O2b antisera. O2a was dominant in all fish speeies. but in eod. the relative number of O2b isolates was eonsiderably higher than in other fish speeies.

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“…Other pathogenic strains of V. anguillarum belonging to serotype 01 that were isolated from cultured fish on the northwestern coast of Spain also harbor a plasmid which exhibits structural and functional homology with pJM1, as assessed by hybridization analysis and curing experiments (28). This 65-kb plasmid seems to be widespread in many strains belonging to serotype 01 (22,35).…”

mentioning

confidence: 99%

Distribution of plasmid- and chromosome-mediated iron uptake systems in Vibrio anguillarum strains of different origins

Conchas

Lemos

Barja

et al. 1991

Appl Environ Microbiol

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We investigated the incidence of plasmid-mediated and chromosome-mediated iron uptake systems in strains of Vibrio anguillarum that belong to serotypes 01 and 02 and were isolated from different fish species and in different geographic areas. All of the strains gave positive reactions in CAS agar medium and in the Arnow test, which indicated that catechol types of siderophores were produced. The majority of V. anguillarum serotype 01 strains harbored a 65-kb plasmid similar to plasmid pJMl from strain 775, which encodes the siderophore anguibactin and its outer membrane receptor, protein OM2. All of the isolates harboring this plasmid promoted the growth of an anguibactin-deficient receptor-proficient mutant derived from strain 775, but none of these isolates promoted the growth of mutants lacking receptor OM2. Furthermore, under iron-limiting conditions all of these strains induced outer membrane proteins that were identical in size to protein OM2 of strain 775. In contrast, none of the serotype 02 strains contained a high-molecular-weight plasmid, but all of them induced the growth of mutants defective in the anguibactin-mediated system regardless of the presence or absence of receptor OM2. The serotype 02 strains, but not the plasmid-bearing serotype 01 strains, also induced the growth of Salmonella typhimurium enb-1 which utilizes only enterobactin as a siderophore. Moreover, the pattern of iron-regulated outer membrane proteins in serotype 02 isolates was totally different from the pattern in the serotype 01 strains harboring the 65-kb plasmid. Interestingly, several plasmidless strains belonging to serotype 01 behaved in the same way as serotype 02 strains in cross-feeding experiments and produced the same iron-regulated outer membrane protein patterns. These results indicate that regardless of their origins all V. anguillarum strains belonging to serotype 02 contain similar chromosome-mediated iron-sequestering systems. However, in the serotype 01 strains both chromosome-and plasmid-encoded iron uptake systems occur, but there is a significant predominance of the plasmid-mediated system.

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Relationships between plasmids and phenotypes of presumptive strains of Vibrio anguillarum isolated from different fish species

Cited by 38 publications

References 31 publications

Restriction fragment length polymorphism of the Vibrio anguillarum serovar O1 virulence plasmid

Restriction fragment length polymorphism of the Vibrio anguillarum serovar O1 virulence plasmid

Vibrio anguillarum serovars associated with vibriosis in fish

Distribution of plasmid- and chromosome-mediated iron uptake systems in Vibrio anguillarum strains of different origins

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