Relationships Between Strains of Mycoplasma mycoides subspp. mycoides and capri Studied by Two-dimensional Gel Electrophoresis of Cell Proteins

Rodwell, A. W.; Es, Rodwell

doi:10.1099/00221287-109-2-259

Cited by 43 publications

(11 citation statements)

References 12 publications

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“…capripneumoniae represent the six recognized members of the M. mycoides cluster, and species identification within this cluster has been problematic. Electrophoretic, immunoblotting and DNA hybridization studies confirm the close phylogenetic relationship between members of the cluster (Bonnet et al, 1993;Christiansen & Ernø, 1990;Costas et al, 1987;Olsson et al, 1990;Rodwell, 1982;Rodwell & Rodwell, 1978) and serological cross-reactions between Mycoplasma sp. bovine group 7 and M. capricolum subsp.…”

Section: Introductionmentioning

confidence: 72%

Characterization of a chromosomal region of Mycoplasma sp. bovine group 7 strain PG50 encoding a glycerol transport locus (gtsABC)

2003

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Mycoplasma species bovine group 7, represented by the type strain PG50, is one of six members of the Mycoplasma mycoides cluster and has been implicated in sporadic and outbreak cases of polyarthritis and mastitis in Australian dairy cattle. This study describes cloning and sequencing a 7?9 kb region of the PG50 chromosome and identification of genes involved in glycerol transport (gtsA, gtsB and gtsC) that are followed by a putative lipoprotein gene lppB and a genomic locus containing two ORFs encoding putative membrane proteins. Long range PCR using primers spanning gtsABC and downstream flanking genes, and Southern hybridization analyses using a suite of probes derived from M. mycoides subsp. mycoides small colony type (SC) strain Afadé for gtsA, gtsB and gtsC, lppB and the two downstream genes confirmed that these genes were conserved among Mycoplasma sp. bovine group 7 isolates and mycoplasmas belonging to the M. mycoides subcluster [M. mycoides subsp. mycoides SC, M. mycoides subsp. mycoides large colony type (LC) and M. mycoides subsp. capri ] but were absent in mycoplasmas belonging to the Mycoplasma capricolum subcluster (M. capricolum subsp. capricolum and M. capricolum subsp. capripneumoniae). M. capricolum subsp. capricolum type strain California kid did not hybridize with the probe for gtsA and gave only weak or no hybridization signals with probes derived from the loci downstream of gtsABC, suggesting that this region has diverged in mycoplasmas belonging to subspecies of M. capricolum. It is shown that PG50, after the addition of a physiological concentration of glycerol to the growth medium, generates H 2 O 2 at levels comparable with strain Afadé , implying that the glycerol transport system is functional in Mycoplasma sp. bovine group 7. This suggests that in PG50, as in M. mycoides subsp. mycoides SC, glycerol uptake is followed by phosphorylation to glycerol 3-phosphate and then conversion to dihydroxyacetone phosphate, catalysed by L-a-glycerophosphate oxidase, resulting in the production of H 2 O 2 . The ability of Mycoplasma sp. bovine group 7 to generate significant amounts of hydrogen peroxide may be important in pathogenesis.

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Section: Introductionmentioning

confidence: 72%

Characterization of a chromosomal region of Mycoplasma sp. bovine group 7 strain PG50 encoding a glycerol transport locus (gtsABC)

2003

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“…It must also be considered that functionally equivalent proteins differing by only a single charged amino acid may appear as unique protein spots when analysed by 2-DE. In fact, bacterial strains can sometimes show a greater degree of variability by 2-DE than observed by DNA-DNA hybridisation [53]. Jackson et al showed that the differences in the 2-D protein profiles observed for these bacteria followed their generally recognised taxonomic classification [41].…”

Section: The Analysis Of Genetic Variabilitymentioning

confidence: 99%

Proteomics in medical microbiology

Cash

2000

Electrophoresis

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“…capricolum [12] have been reported. Two-dimensional electrophoretic, immunoblotting, and DNA hybridisation studies also suggest a close relationship among species of the M. mycoides group [13][14][15][16][17][18]. Studies by Rodwell, [14] showed that the protein profiles of ten isolates of Mycoplasma sp.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity amongMycoplasmaspecies bovine group 7: Clonal isolates from an outbreak of polyarthritis, mastitis, and abortion in dairy cattle

et al. 2001

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A comprehensive genetic analysis of 60 Mycoplasma sp. bovine group 7 isolates from different geographic origins and epidemiological settings is presented. Twenty-four isolates were recovered from the joints of calves during sporadic episodes of polyarthritis in geographically distinct regions of Queensland and New South Wales, Australia, including two clones of the type strain PG5O. A further three Australian isolates were also recovered from the tympanic bulla, retropharyngeal lymph node and the lung and another three isolates had unconfirmed histories. Six isolates originated from Germany, Portugal, Nigeria, and France. Twenty-four epidemiologically related isolates of Mycoplasma sp. bovine group 7 were recovered from multiple tissue sites and body fluids of infected calves with polyarthritis, mastitic milk, and from the stomach contents, lung and liver from aborted foetuses in three large, centrally managed dairy herds in New South Wales, Australia. Restriction endonuclease analysis (REA) of genomic DNA differentiated 29 Cfol profiles among these 60 isolates and grouped all 24 epidemiologically related isolates in a defined pattern showing a clonal origin. Three isolates of this clonal cluster were recovered from mastitic milk and the synovial exudate of clinically-affected calves and appeared sporadically for periods up to 18 months after the initial outbreak of polyarthritis indicating a persistent, close association of the organism with cattle in these herds. The Cfol profile representative of the clonal cluster was distinguishable from profiles of isolates recovered from multiple, unrelated cases of polyarthritis in Queensland and New South Wales and from other countries. All 24 isolates from the clonal cluster possessed a plasmid (pBG7AU) with a molecular size of 1022 bp. DNA sequence analysis of pBG7AU identified two open reading frames sharing 81 and 99% DNA sequence similarity with hypothetical replication control proteins A and B respectively, previously described in plasmid pADB201 isolated from M. mycoides subspecies mycoides. Other isolates of bovine group 7, epidemiologically unrelated to the clonal cluster, including two clones of the type strain PG5O, possessed a similar-sized plasmid. These data confirm that Mycoplasma sp. bovine group 7 is capable of migrating to, and multiplying within, different tissue sites within a single animal and among different animals within a herd.

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Relationships Between Strains of Mycoplasma mycoides subspp. mycoides and capri Studied by Two-dimensional Gel Electrophoresis of Cell Proteins

Cited by 43 publications

References 12 publications

Characterization of a chromosomal region of Mycoplasma sp. bovine group 7 strain PG50 encoding a glycerol transport locus (gtsABC)

Characterization of a chromosomal region of Mycoplasma sp. bovine group 7 strain PG50 encoding a glycerol transport locus (gtsABC)

Proteomics in medical microbiology

Genetic diversity amongMycoplasmaspecies bovine group 7: Clonal isolates from an outbreak of polyarthritis, mastitis, and abortion in dairy cattle

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