Relative expression of seven candidate genes for pathogen resistance on Pinus radiata infected with Fusarium circinatum

Donoso, Andrea; Rodríguez, Victoria; Carrasco, Angela; Ahumada, Rodrigo; Sanfuentes, Eugenio; Valenzuela, Sofía

doi:10.1016/j.pmpp.2015.08.009

Cited by 26 publications

(28 citation statements)

References 71 publications

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“…Transcriptome analysis of Pinus radiata inoculated seedlings was recently published [18] showing induction of genes related to abscisic acid (ABA) signaling, auxin responsive-like proteins, gibberellin-regulated protein precursor, as well as induction of pathogenesis-related (PR) proteins, phosphorylase family protein (PFP) and genes related to physical and chemical barriers to restrict pathogen invasion. Similarly, Donoso et al, [32] detected an upregulation of genes encoding thaumatin-like protein (PR5) in P. radiata inoculated seedlings by a RT-qPCR assay. Davis et al, [27] reported SA and JA induction of chitinase (PR3) in pine seedlings inoculated with Fusarium subglutinans f. sp.…”

Section: Introductionmentioning

confidence: 84%

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The transcriptome of Pinus pinaster under Fusarium circinatum challenge

et al. 2020

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Background: Fusarium circinatum, the causal agent of pitch canker disease, poses a serious threat to several Pinus species affecting plantations and nurseries. Although Pinus pinaster has shown moderate resistance to F. circinatum, the molecular mechanisms of defense in this host are still unknown. Phytohormones produced by the plant and by the pathogen are known to play a crucial role in determining the outcome of plant-pathogen interactions. Therefore, the aim of this study was to determine the role of phytohormones in F. circinatum virulence, that compromise host resistance. Results: A high quality P. pinaster de novo transcriptome assembly was generated, represented by 24,375 sequences from which 17,593 were full length genes, and utilized to determine the expression profiles of both organisms during the infection process at 3, 5 and 10 days post-inoculation using a dual RNA-sequencing approach. The moderate resistance shown by Pinus pinaster at the early time points may be explained by the expression profiles pertaining to early recognition of the pathogen, the induction of pathogenesis-related proteins and the activation of complex phytohormone signaling pathways that involves crosstalk between salicylic acid, jasmonic acid, ethylene and possibly auxins. Moreover, the expression of F. circinatum genes related to hormone biosynthesis suggests manipulation of the host phytohormone balance to its own benefit. Conclusions: We hypothesize three key steps of host manipulation: perturbing ethylene homeostasis by fungal expression of genes related to ethylene biosynthesis, blocking jasmonic acid signaling by coronatine insensitive 1 (COI1) suppression, and preventing salicylic acid biosynthesis from the chorismate pathway by the synthesis of isochorismatase family hydrolase (ICSH) genes. These results warrant further testing in F. circinatum mutants to confirm the mechanism behind perturbing host phytohormone homeostasis.

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Section: Introductionmentioning

confidence: 84%

“…While most pine studies have focused their attention on host defense [18,32], little is known about F. circinatum genes involved in pathogenicity. Muñoz-Adalia et al [70] suggested 5 candidate genes that could be involved in F. circinatum virulence based on high similarity with other Fusarium species.…”

Section: Introductionmentioning

confidence: 99%

The transcriptome of Pinus pinaster under Fusarium circinatum challenge

et al. 2020

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“…But this history of potential success is not free of significant drawbacks that need careful consideration. To properly exploit genetic resistance against F. circinatum , special attention should be paid to (i) improving and standardizing the current screening protocols (Oak et al ., ; Roux et al ., ); (ii) developing genomic selection protocols (Quesada et al ., ; Moraga‐Suazo et al ., ; Donoso et al ., ; Carrasco et al ., ); (iii) discovery of durable and stable resistance operational across different environmental conditions, F. circinatum strains and tree ontogenetic stages; (iv) quantifying the consequences of putative trade‐offs between resistance and other life functions (e.g. growth–defence trade‐off) (Matheson et al ., ; Vivas et al ., ); and (v) exploration of other sources of variation with genetic backgrounds, such as inducibility (Agrawal et al ., ; Moreira et al ., ), maternal effects (Vivas et al ., ) or the attraction and susceptibility of trees to vectoring insects (Erbilgin et al ., ).…”

Section: Exploiting Genetic Resistance Against F Circinatummentioning

confidence: 99%

Environmentally friendly methods for controlling pine pitch canker

et al. 2019

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Fusarium circinatum is a highly virulent invasive pathogen that causes the disease commonly known as pine pitch canker (PPC). On mature trees, the most common symptoms of PPC include resin bleeding, resinous cankers, wilting of needles and dieback. In nurseries the main symptoms are yellowish needles, and wilting of foliage and shoots. PPC is considered one of the most important diseases of conifers globally and at least 60 species of Pinus along with Pseudotsuga menziesii are known to be susceptible. Quarantine regulations are crucial to minimize the risk of new introductions into disease‐free countries. However, if these measures fail, implementation of environmentally friendly control methods is currently encouraged in an integrated management approach that minimizes the use of chemicals in forests. This review therefore summarizes current knowledge of biological control using endophytic fungi, bacteria and viruses, and other environmentally friendly methods to control PPC, including thermotherapy, natural products with fungicidal effects, promoters of innate host resistance mechanisms and breeding for resistance. Key research gaps to be addressed for improvement of effective management of this disease are discussed.

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“…Thus it allows the spread of F. circinatum (Donoso et al, 2015), causing the death of seedlings (Fitza et al, 2013). So far, the pathogen has shown no evidence of susceptibility to and biological control measures (Donoso et al, 2015).…”

Section: Pitch Cankermentioning

confidence: 99%

The fungal pathogens causing diseases in pines

Raitelaitytė¹,

Rutkauskas²,

Radzijevskaja³

et al. 2017

biologija

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Fungal infections are the main cause of emerging infectious diseases in forest trees. Over the past decades, the number of invasive fungal pathogens in Europe has increased exponentially. In this paper the fungal pathogens causing the most common diseases in pines like Dothistroma needle blight, brown spot needle blight, Lophodermium needle cast, Scots pine blister rust, Scleroderris canker, and Pitch canker were analyzed. These diseases cause defoliation, increase susceptibility of plants to other diseases and pests, and tree mortality can also occur. As a result, the forest industry is suffering severe economic losses. The fungi species causing infection in forest trees have been described as serious pathogens across the world including Europe, confirming a fast spread of their ranges. Knowledge of pathogens distribution, life cycle and disease symptoms are essential for the diagnostic and control of pathogenic fungi. Human-driven species expansion has increased in the last century due to the growth of international travel and trade, resulting in huge disturbance to ecosystems. Most of the plant diseases are strongly influenced by environmental conditions. Climate change has important consequences on plants, pathogens, and the interaction between them, resulting in changes on diseases epidemics. Fungal infections of plants are difficult to control because pathogens populations are variable in time, space, and genotypes. The potential damage in the future may be large, and that is why we have to be aware of the problems and discuss some possible approaches to reducing the threats.

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Relative expression of seven candidate genes for pathogen resistance on Pinus radiata infected with Fusarium circinatum

Cited by 26 publications

References 71 publications

The transcriptome of Pinus pinaster under Fusarium circinatum challenge

The transcriptome of Pinus pinaster under Fusarium circinatum challenge

Environmentally friendly methods for controlling pine pitch canker

The fungal pathogens causing diseases in pines

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