Relative importance of <i>Microcystis</i>
 abundance and diversity in determining microcystin dynamics in Lake Erie coastal wetland and downstream beach water

Hu, Chenlin; Rea, Chris L.; Yu, Zhongtang; Lee, Ji-Young

doi:10.1111/jam.12983

Cited by 30 publications

(19 citation statements)

References 32 publications

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“…MCs were extracted from Microcystis aeruginosa (identified by targeting PC-IGS and mcyA genes [38]) that was isolated from Lake Erie. M. aeruginosa was cultured in CT medium [39] using sonication (three cycles of 5-min sonication followed by 10-min rest) with a Sonic Dismembrator (50 watts, Fisher Scientific Model F50 with Probe, Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Sustainable Methods for Decontamination of Microcystin in Water Using Cold Plasma and UV with Reusable TiO2 Nanoparticle Coating

Jiang

Lee

Mok

et al. 2017

IJERPH

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Microcystins (MCs) are a family of cyanotoxins and pose detrimental effects on human, animal, and ecological health. Conventional water treatment processes have limited success in removing MCs without producing harmful byproducts. Therefore, there is an urgent need for cost-effective and environmentally-friendly methods for treating MCs. The objective of this study was to develop sustainable and non-chemical-based methods for controlling MCs, such as using cold plasma and ultra violet (UV) light with titanium dioxide (TiO2) coating, which can be applied for diverse scale and settings. MCs, extracted from Microcystis aeruginosa, were treated with cold plasma or UV at irradiance of 1470 μW/cm2 (high) or 180 μW/cm2 (low). To assess synergistic effects, the outside of the UV treatment chamber was coated with nanoparticles (TiO2) prior to irradiation, which can be reused for a long time. The degradation efficiency of UV was enhanced by the reusable TiO2 coating at lower irradiance (70.41% [UV] vs. 79.61% [UV+TiO2], 120 min), but no significant difference was observed at higher irradiance. Cold plasma removed MCs rapidly under experimental conditions (92%, 120 min), indicating that it is a promising candidate for controlling MCs in water without generating harmful disinfection byproducts. It can be also easily and practically used in household settings during emergency situations.

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Section: Methodsmentioning

confidence: 99%

Sustainable Methods for Decontamination of Microcystin in Water Using Cold Plasma and UV with Reusable TiO2 Nanoparticle Coating

Jiang

Lee

Mok

et al. 2017

IJERPH

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“…Freshwater bacteria have been extensively investigated via culture-independent methods, such as fluorescence in situ hybridization (FISH) method (Sekar et al, 2003; Lindström et al, 2005). Terminal restriction fragment length polymorphism (T-RFLP) and quantitative polymerase chain reaction (qPCR) (Eiler and Bertilsson, 2004, 2007; Hu et al, 2016) were used for studying diversity of freshwater bacterial communities associated with cyanobacterial bloom. The application of culture-independent methods provided information on bacterial diversity in freshwaters that had been underestimated in the past.…”

Section: Introductionmentioning

confidence: 99%

The Microbiota of Recreational Freshwaters and the Implications for Environmental and Public Health

et al. 2016

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The microbial communities in recreational freshwaters play important roles in both environmental and public health perspectives. In this study, the bacterial community structure and its associations with freshwater environments were investigated by analyzing the summertime microbiomes of three beach waters in Ohio (East Fork, Delaware, and Madison lakes) together with environmental and microbial water quality parameters. From the swimming season of 2009, 21 water samples were collected from the three freshwater beaches. From the samples, 110,000 quality-checked bacterial 16S rRNA gene sequences were obtained and analyzed, resulting in an observation of 4500 bacterial operational taxonomic units (OTUs). The most abundant bacteria were Mycobacterium and Arthrobacter of the Actinobacteria (33.2%), Exiguobacterium and Paenisporosarcina of the Firmicutes (23.4%), Planktothrix and Synechococcus of the Cyanobacteria (20.8%), and Methylocystis and Polynucleobacter of the Proteobacteria (16.3%). Considerable spatial and temporal variations were observed in the bacterial community of Actinobacteria, Cyanobacteria, and Firmicutes, where the bacterial community structure was greatly influenced by hydrological and weather conditions. The most influential factors were (1) water inflow for Bacteroidia and Clostridia, (2) turbidity for Gammaproteobacteria, (3) precipitation for Bacilli, and (4) temperature and pH for Cyanobacteria. One noticeable microbial interaction in the bacterial community was a significant negative relationship between Cyanobacteria and Bacilli (P < 0.05). Concerning beach water quality, the level of the genetic markers for cyanobacterial toxin (mcyA) was linked to the abundance of Cyanobacteria. In addition, unique distributions of the genera Enterococcus, Staphylococcus, Streptococcus, Bacteroides, Clostridium, Finegoldia, Burkholderia, and Klebsiella, together with a high density of fecal indicator Escherichia coli, were markedly observed in the sample from Madison Lake on July 13, suggesting a distinctly different source of bacterial loading into the lake, possibly fecal contamination. In conclusion, deep sequencing-based microbial community analysis can provide detailed profiles of bacterial communities and information on potential public health risks at freshwater beaches.

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“…In this sense, Rinta-Kanto and Wilhelm (2006) studied the genetic diversity of potentially toxic Microcystis based on the McyA amino acid sequence and reported new sequences of the mcyA gene (Rinta-Kanto and Wilhelm, 2006). Using mcyA amplification and denaturing gradient gel electrophoresis (DGGE), Hu et al (2016) found that the microcystin variants were related to the band pattern obtained, implying that the composition of Microcystis community determined the kind of microcystin produced (Hu et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…A study in Lake Erie (USA) found that relative abundances of some genotypes changed temporally, indicating the existence of different genotypes adapted to particular environmental characteristics (Hu et al, 2016). In the same ecosystem, Berry et al (2017) analysed Microcystis oligotypes using a computational method (oligotyping) based on the V4 region of the 16S rRNA gene and found changes in Microcystis oligotypes composition related to spatial nutrient gradients.…”

Section: Discussionmentioning

confidence: 99%

Genotyping and functional regression trees reveals environmental preferences of toxic cyanobacteria (Microcystis aeruginosacomplex) along a wide spatial gradient

Escalera

Segura

Kruk

et al. 2019

Preprint

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Addressing ecological and evolutionary processes explaining biodiversity patterns is essential to identify the mechanisms driving community assembly. In the case of bacteria, the formation of new ecologically distinct populations or ecotypes is proposed as one of the main drivers of diversification. New ecotypes arise when mutation in key functional genes or acquisition of new metabolic pathways by horizontal gene transfer allow the population to exploit new resources, making possible their coexistence with parental population. Recently, we have reported the presence of toxic, microcystin-producing organisms from the Microcystis aeruginosa complex (MAC) through a wide environmental gradient (800 km) in South America, ranging from freshwater to estuarine-marine waters. In order to explain this finding, we hypothesize that the success of toxic organisms of MAC in such array of environmental conditions is due to the existence of ecotypes having different environmental preferences. So, we analysed the genetic diversity of microcystin-producing populations of Microcystis aeruginosa complex (MAC) by qPCR and high resolution melting analysis (HRMA) of a functional gene (mcyJ, involved in microcystin synthesis) and explored its relationship with the environmental conditions through the gradient by functional classification and regression trees (fCART). Six groups of mcyJ genotypes were distinguished and selected by different combinations of water temperature, conductivity and turbidity, determining the environmental preferences of each group. Since these groups were based on the basis of similar sequence and ecological characteristics they were defined as ecotypes of toxic MAC. Taking into account that the role of microcystins in MAC biology and ecology has not yet been elucidated, we propose that the toxin might have a role in MAC fitness that would be mainly controlled by the physical environment in a way such that the ecotypes that thrive in the riverine zone of the gradient would be more stable and less influenced by salinity fluctuations than those living at the marine limit of the estuary. These would periodically disappear or being eliminated by salinity increases, depending on the estuary dynamics. Thus, ecotypes generation would be an important mechanism allowing toxic MAC adapting to and succeed in a wide array of environmental conditions.

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Relative importance of Microcystis abundance and diversity in determining microcystin dynamics in Lake Erie coastal wetland and downstream beach water

Cited by 30 publications

References 32 publications

Sustainable Methods for Decontamination of Microcystin in Water Using Cold Plasma and UV with Reusable TiO2 Nanoparticle Coating

Sustainable Methods for Decontamination of Microcystin in Water Using Cold Plasma and UV with Reusable TiO2 Nanoparticle Coating

The Microbiota of Recreational Freshwaters and the Implications for Environmental and Public Health

Genotyping and functional regression trees reveals environmental preferences of toxic cyanobacteria (Microcystis aeruginosacomplex) along a wide spatial gradient

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