Relevance of single-nucleotide polymorphism to the expression of resistin gene affecting serum and gingival crevicular fluid resistin levels in chronic periodontitis and type 2 diabetes mellitus: A randomized control clinical trial

Abstract

Aim:The present study is aimed to investigate whether single-nucleotide polymorphism (SNP) of resistin gene (RETN) at −420 and +299 sites, is associated with resistin levels in serum and gingival crevicular fluid (GCF) in periodontally healthy, chronic periodontitis (CP) with and without type 2 diabetes mellitus (T2DM) patients.Materials and Methods:Serum and GCF samples were procured from all the 60 patients (twenty in each group) of the three study groups i.e., periodontally healthy (Group I), CP (Group II) and CP with T2DM patients (Group III) to analyze resistin levels using enzyme-linked immunosorbent assay test and clinical parameters were assessed at baseline and at 3 months after scaling and root planing (SRP). RETN polymorphism at −420 and +299 was genotyped by polymerase chain reaction-restriction fragment length polymorphism technique.Results:Patients with SNP −420 and +299 were positively correlated with increased serum and GCF resistin levels in Group II and Group III patients. SRP led to substantial reduction in the serum and GCF resistin levels.Conclusion:These findings are suggestive of a biologic link between resistin, periodontal diseases, and periodontal diseases with T2DM and RETN SNP at −420 and +299 in imparting increased resistin levels in inflammatory and diabetic conditions.

Association of RETN +299(G>A) polymorphism with type two diabetes mellitus

“…The +299 (G>A) SNP is located in the second intron of the RETN gene (Suriyaprom et al, 2009). Several explanations may explain the association of this SNP with T2DM Li et al, 2007;Rode et al, 2019;Suriyaprom et al, 2009). For example, this SNP may be in linkage disequilibrium with another SNP that modulates level of gene expression of resistin, similar to that reported between -358 and +420 RETN SNPs (Onuma et al, 2010).…”

The Influence of Fixed Dental Prostheses on the Expression of Inflammatory Markers and Periodontal Status—Narrative Review

“…Immune and epithelial cells both release resistin, a hormone peptide generated from adipose tissue. Resistin can act as a pro-inflammatory mediator and is a key marker for inflammatory disorders, according to recent research [ 47 ]. Human cartilage glycoprotein-39 (YKL-40) is a recently identified inflammatory factor with several tissue origins.…”

Evaluation and association of periodontal status with levels of Porphyromonas gingivalis in chronic periodontitis with and without Type 2 diabetes mellitus following nonsurgical periodontal therapy using quantitative polymerase chain reaction: An interventional study

“…Since it is a follow-up study, the sample size was considered with reference to the previous study. [ 6 ] A dental and medical history was recorded for the selected patients, and an intraoral examination was conducted by a single examiner (RK). The intrarater reliability in the measurements of periodontal parameters was evaluated before treatment in the study groups.…”

“…[ 5 ] NSPT was performed to evaluate the reduction in inflammatory status with an interval of 3 months in our previous study. [ 6 ] Although significantly positive results were obtained when evaluated clinically to know whether eradication of causative microbes and complete remission of disease could be achieved after 3 months of NSPT, led us to generate a further protocol involving microbiological analysis.…”