Remote ischemic perconditioning attenuates ischemia/reperfusion-induced downregulation of AQP2 in rat kidney

Kristensen, Marie Louise Vindvad; Kierulf-Lassen, Casper; Nielsen, Per Mose; Krag, Susanne; Birn, Henrik; Nejsum, Lene N.; Nørregaard, Rikke

doi:10.14814/phy2.12865

Cited by 19 publications

(17 citation statements)

References 45 publications

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“…Urine osmolality in the I-R group significantly declined compared to the control group due to impaired urinary concentrating ability. Unsuitable regulation of the AQPs and Na + transporter proteins in renal tubules is responsible for changes in sodium and water regulation under the influence of ischemia-reperfusion injury [23]. Urine osmolarity in the NG group was not significantly different from that of the I-R group.…”

Section: Discussionmentioning

confidence: 84%

Effects of Nitroglycerine on Renal Ischemia-Reperfusion Injury in Adult Male Rats

et al. 2019

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Background Ischemia-reperfusion (I-R) leads to acute kidney injury (AKI). The present study investigated the effects of nitroglycerine (NG) on improving renal dysfunctions caused by I-R in rats. Methodology Twenty-four rats were equally divided into four groups: (1) the control group, (2) the sham group, (3) the I-R group, and (4) NG-treated groups.NG (50 μg/kg) was injected intraperitoneally after induction of IR. I-R was induced through clamping of the bilateral renal artery and vein of both kidneys for 20 min followed by 24 h of reperfusion. Results NG significantly increased the creatinine clearance levels and renal blood flow rate (which was reduced by I-R). NG also significantly improved serum electrolytes (sodium and potassium) that were disordered by I-R. In addition, NG significantly offset impaired antioxidant defense mechanism and inhibited lipid peroxidation. Conclusions The results show NG has a protective effect on renal tissue against AKI caused by I-R. These protective effects mediated through antioxidant activity and decrease of lipid peroxidation.

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Section: Discussionmentioning

confidence: 84%

Effects of Nitroglycerine on Renal Ischemia-Reperfusion Injury in Adult Male Rats

et al. 2019

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“…Inappropriate regulation of the AQPs and Na + transporter proteins in renal tubules and decreasing the expression of Na + /K + -ATPase in the thick descending limb of the external medulla that causing changes in sodium and water regulation under the influence of I-R injury. 19 In the hydralazine-treated rats, urinary osmolality was significantly more than that of the I-R rats. Previous studies have shown that hydralazine reduces urinary volume due to water and sodium retention.…”

Section: Discussionmentioning

confidence: 84%

“…30 In addition, I-R causes brush border destruction with reduced reabsorption of sodium by proximal tubules, impaired expression of tubular sodium transporters and unsuitable regulation of Na + /K + -ATPase expression in the basolateral membranes. 19,30 In the I-R injury lead to direct injury of cells responsible for the secretion of potassium in the distal tubules and collecting ducts. 31 Post-treatment of hydralazine in the I-R rats reduced urinary excretion of sodium compared to the I-R rats.…”

Section: Discussionmentioning

confidence: 99%

“…Osmolarity of urine and plasma samples was measured using a Osmometer (Gonotec Osmomat-030, Germany). 18,19 Renal clearance of creatinine (C Cr ), absolute and relative excretions of potassium and sodium was calculated using the following formula: C Cr (μl/min/gkw) = (V°/1000 × U Cr )/P Cr Absolute excretion of sodium: U Na V°(μmol/min/gkw) = (V° × U Na )/1000.…”

Section: Methodsmentioning

confidence: 99%

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Therapeutic Effects of Hydralazine on Renal Ischemia-Reperfusion Injury in Rat.

2019

J. contemp. med. sci.

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Objective Renal ischemia-reperfusion (I-R) induces acute kidney injury (AKI). In this study, the effect of hydralazine was investigated on the renal injury induced by the I-R in rats. Methods AKI was induced with bilateral obstruction of the renal artery and vein for 20 min following 24 h of reperfusion. Hydralazine (5mg/ kg) was injected intraperitoneally as post-treatment. Results Hydralazine significantly increased the levels of renal clearance of creatinine and renal blood flow, while they were decreased by I-R. Also, hydralazine significantly improved levels of serum electrolytes (sodium and potassium) that were impaired by I-R. The tissue malondialdehyde levels were significantly suppressed by hydralazine. Conclusion According to the results, the post-treatment of hydralazine had a therapeutic effect on renal I-R because of improved ion reabsorption and excretion and increased renal blood flow and glomerular filtration rate and decreased lipid peroxidation. Key words hydralazine, renal ischemia-reperfusion injury, rat This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.

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“…AQP2 was first discovered in the early 1990s, it is a member of the aquaporin family (16). AQP2 mainly exists in the cytoplasm of principal cell of renal collecting duct and the cell membrane towards the luminal side, which can increase the ability of water reabsorption and urine concentration (17). It was indicated in the results of this study that ALB level in children with hydronephrosis was significantly lower than that in normal children and it was significantly increased after treatment (P<0.05).…”

Section: Discussionmentioning

confidence: 99%

Diagnostic value of urinary RBP, ALB and AQP2 in neonatal hydronephrosis and the relationship with expression of MCP-1 in the prenatal maternal peripheral blood

et al. 2018

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Diagnostic value of urinary retinol binding protein (RBP), albumin (ALB) and aquaporin-2 (AQP2) in neonatal hydronephrosis and their relationship with the expression of monocyte chemoattractant protein 1 (MCP-1) in the prenatal maternal peripheral blood was investigated. Forty-six child patients with hydronephrosis admitted to Hongqi Hospital Affiliated to Mudanjiang Medical College from December 2016 to November 2017 were selected as the observation group and the control included 46 normal newborn infants. The urinary RBP, ALB, AQP2 and the expression of MCP-1 in the prenatal maternal peripheral blood in the two groups were compared. The diagnostic value of the combination of urinary RBP, ALB and AQP2 for the neonatal hydronephrosis was accessed through the area under curve (AUC). The changes of urinary RBP, ALB and AQP2 of child patients were observed and the correlations between RBP, ALB, AQP2 and MCP-1 were analyzed. The concentrations of RBP and ALB in the observation group were obviously increased compared to those in the control group. The AQP2 concentration in the observation group was lower than that in the control group. In the observation group, the MCP-1 level in the prenatal maternal blood was significantly higher than that in the control group (P<0.05). After treatment, the concentration of RBP and ALB in the child patients were significantly decreased and AQP2 concentration was increased compared with that before treatment (P<0.05). The AUC of the diagnosis combining with RBP, ALB and AQP2 was 0.913. RBP and ALB were positively correlated to MCP-1 in the prenatal maternal peripheral blood and there was a negative correlation between AQP2 and MCP-1 (P<0.05). In conclusion, urinary RBP, ALB and AQP2 can be regarded as markers for the diagnosis of the neonatal hydronephrosis and they are also closely related to the MCP-1 level in the prenatal maternal peripheral blood.

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Remote ischemic perconditioning attenuates ischemia/reperfusion-induced downregulation of AQP2 in rat kidney

Cited by 19 publications

References 45 publications

Effects of Nitroglycerine on Renal Ischemia-Reperfusion Injury in Adult Male Rats

Effects of Nitroglycerine on Renal Ischemia-Reperfusion Injury in Adult Male Rats

Therapeutic Effects of Hydralazine on Renal Ischemia-Reperfusion Injury in Rat.

Diagnostic value of urinary RBP, ALB and AQP2 in neonatal hydronephrosis and the relationship with expression of MCP-1 in the prenatal maternal peripheral blood

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