Remote Tuning of Built‐In Magnetoelectric Microenvironment to Promote Bone Regeneration by Modulating Cellular Exposure to Arginylglycylaspartic Acid Peptide

Abstract

Mimicking the endogenous physical microenvironment is a promising strategy for biomaterial‐mediated tissue regeneration. However, precise control of physical cues such as electric/magnetic fields within extracellular environments to facilitate tissue regeneration remains a formidable challenge. Here, remote tuning of the magnetoelectric microenvironment is achieved by a built‐in CoFe2O4/poly(vinylidene fluoridetrifluoroethylene) [P(VDF‐TrFE)] magnetoelectric membrane for effective bone regeneration. The magnetoelectric microenvironment from the nanocomposite membranes promotes osteogenic differentiation of bone marrow mesenchymal stem cells (BM‐MSCs) and enhances bone defect regeneration by increasing cellular exposure and integrin binding to arginylglycylaspartic acid peptide, as predicted by molecular dynamics simulations. Moreover, BM‐MSCs are directed to the osteogenic lineage by osteoimmuomodulation which involves accelerating transition from an initial inflammatory immune response to a pro‐healing regenerative immune response. This work offers a strategy to mimic the magnetoelectric microenvironment for achieving precise and effective tissue regenerative therapies, as well as provides fundamental insights into the biological effects driven by the built‐in magnetoelectric membrane, which can be remotely tuned to precisely modulate osteogenesis in situ.

Mechanistically Scoping Cell‐Free and Cell‐Dependent Artificial Scaffolds in Rebuilding Skeletal and Dental Hard Tissues

“…[267] Due to the highly tissue-penetrative and biocompatible, remote, and nonintrusive magnetic stimulation via applying external electromagnetic field was reported to be helpful for bone engineering. [268][269][270] It was found that the mobility of RGD peptide functionalized magnetic nanoparticles was regulated by external magnetic field, thereby affecting vinculin and YAP functions. In contrast to RGD with high mobility, the confined RGD promoted osteogenic differentiation of MSCs.…”

Tannin-bridged magnetic responsive multifunctional hydrogel for enhanced wound healing by mechanical stimulation-induced early vascularization

“…As a non-invasive and convenient treatment tool, magnetic fields have started to be applied in tissue engineering and repair. 9,10 At present, there are four main types of magnetic field: pulsed electromagnetic fields, static magnetic fields (SMF), rotating magnetic fields, and alternating electromagnetic fields. 11 In this work, a SMF was selected as a tool to assist injury healing.…”

“…15 The National Center for Complementary and Alternative Medicine (NCCAM) does, however, consider the low SMF produced by neodymium and ferrite magnets to be safe, and the scientific community is urged to examine it for therapeutic uses. Relevant studies have shown that SMFs with different intensity (10,50,80, 100 mT) had no effect on the survival rate of mouse embryonic fibroblasts and caused no damage to the cell membrane. 11 Therefore, it is vital to find a solution to the challenge of how to efficiently stimulate wound healing under lower SMFs.…”

The osteogenic response to chirality-patterned surface potential distribution of CFO/P(VDF-TrFE) membranes

“…Liu et al constructed a CoFe 2 O 4 /poly(vinylidene fluoride-trifluoroethylene (CFO/P(VDF-TrFE)) electroactive membrane to effectively promote the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and the regeneration of bone defects in vivo. 2 The reason for piezoelectric biomaterials to promote osteogenesis is believed to provide a suitable endogenous electrophysiological microenvironment. 3 The intrinsic potential intensity, 4 directional structure arrangement 5 and dynamic enhancement on the surface of piezoelectric biomaterials 6 have been used to participate in the construction of a suitable electrophysiological microenvironment to regulate the cell membrane potential, 7 membrane receptor proteins and extracellular matrix, 8 and then mediate cellular osteogenic differentiation behavior.…”