Removal of estrogenic activity of 4‐tert‐octylphenol by ligninolytic enzymes from white rot fungi

Abstract

4-tert-Octylphenol (4-t-OP) was treated with the white rot fungus Phanerochaete sordida YK-624 under ligninolytic condition with low-nitrogen and high-carbon culture medium. 4-t-OP completely disappeared after 5 days of treatment and the activities of ligninolytic enzymes, laccase and manganese peroxidase (MnP), were detected during this period, thus suggesting that the disappearance of 4-t-OP is related to these extracellular enzymes. Therefore, 4-t-OP was treated with laccase and MnP prepared from white rot fungi cultures. HPLC analysis demonstrated that 4-t-OP completely disappeared in the reaction mixture after 1 h of treatment with either laccase or MnP. Using the yeast two-hybrid assay system, it was also confirmed that laccase and MnP substantially removed the estrogenic activity of 4-t-OP after 1 and 2 h of treatment, respectively. These results strongly demonstrate that ligninolytic enzymes are effective in removing the estrogenic activity of 4-t-OP.

Removal of estrogenic activity of iso-butylparaben and n-butylparaben by laccase in the presence of 1-hydroxybenzotriazole

“…3). These results suggest that degradation of parabens by laccase itself is possible, but is not sufficiently effective for application to the bioremediation of parabens, although several studies on laccase treatment of endocrine-disrupting (estrogenic) chemicals have demonstrated its efficiency (Tamagawa et al 2005(Tamagawa et al , 2006(Tamagawa et al , 2007Kim et al 2008;Sei et al 2008).…”

“…There is currently great interest in these fungi and their ligninolytic enzymes due to their potential for degrading and detoxifying recalcitrant environmental pollutants, such as polychlorinated dioxins (Kamei et al 2005), chlorophenols (Ehlers and Rose 2005), polycyclic aromatic hydrocarbons (Cambria et al 2008), and dyes (Asgher et al 2008). Furthermore, we recently demonstrated that ligninolytic enzymes such as manganese peroxidase (MnP) and laccase, which are produced extracellularly by white rot fungi, are effective in removing the estrogenic activities of nonylphenol (NP), bisphenol A (BPA), 4-tert-octylphenol, 17b-estradiol (E 2 ), ethinylestradiol, estrone, and genistein (Tsutsumi et al 2001;Suzuki et al 2003;Tamagawa et al 2005Tamagawa et al , 2006Tamagawa et al , 2007, and in degrading methoxychlor ) and the antifouling compound Irgarol 1051 (Ogawa et al 2004). These studies prompted investigation into removing the estrogenic activity of parabens using white rot fungi and ligninolytic enzymes, which are able to degrade various aromatic compounds.…”

Removal of diclofenac and mefenamic acid by the white rot fungus Phanerochaete sordida YK-624 and identification of their metabolites after fungal transformation

“…There is currently great interest in these fungi and their ligninolytic enzymes due to their potential for degrading and detoxifying recalcitrant environmental pollutants, such as polychlorinated dioxins (Kamei et al 2005), chlorophenols (Ehlers and Rose 2005), polycyclic aromatic hydrocarbons (Cambria et al 2008) and dyes (Asgher et al 2008). We recently demonstrated that ligninolytic enzymes such as manganese peroxidase (MnP) and laccase, which are produced extracellularly by white rot fungi, are effective in removing the estrogenic activities of bisphenol A, nonylphenol, 4-tert-octylphenol, 17b-estradiol, ethinylestradiol, genistein, iso-butylparaben and n-butylparaben (Tsutsumi et al 2001;Suzuki et al 2003;Tamagawa et al 2005Tamagawa et al , 2007Mizuno et al 2009). Furthermore, recent studies showed the capability of white rot fungi to degrade the pharmaceuticals such as ibuprofen, ketoprofen, clofibric acid, carbamazepine, atenolol and propranolol (Marco-Urrea et al 2009a, b, c).…”

Degradation of endocrine disrupting chemicals by genetic transformants with two lignin degrading enzymes in Phlebia tremellosa

“…These enzymes are also involved in the degradation of many recalcitrant chemicals such as dyes (Champagne and Ramsay, 2005), EDCs (Cabana et al, 2007;Tamagawa et al, 2007), explosives (Cheong et al, 2006) and pesticides (Rezenda et al, 2005;Maruyama et al, 2006). A white rot fungus Phlebia tremellosa has been used in the degradation of EDCs (Yeo et al, 2008a), and its purified laccase was reported to have this degrading activity (Kim et al, 2008).…”