Renal effects of potassium dichromate in the rat: Comparison of urinary enzyme excretion with corresponding tissue patterns

Ngaha, Edwin O.

doi:10.1016/0306-3623(81)90078-1

Cited by 17 publications

(16 citation statements)

References 8 publications

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“…ALP is a membrane bound enzyme while ALT and AST are cytosolic enzymes. These enzymes are highly concentrated in the liver and kidney and are only found in the serum in significant quantities when the cell membrane becomes leaky and even completely ruptured (Cotran et al, 1989;Ngaha, 1981). A rise in serum level or decrease in tissue level of these intracellular enzymes is an index of damage to liver and kidney cells (Moss and Rosalki, 1986).…”

Section: Discussionmentioning

confidence: 99%

Kidney and Liver Function Parameters in Alloxan-Induced Diabetic Rats Treated with Aloe Barbadensis Juice Extract

A¹,

Oluwole²,

Babara³

2010

Sierra Leone j. biomed. res.

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Aloe barbadensis juice extract has been reported to possess hypoglycaemic property but the effects of its use on kidney and liver functions in diabetic animals have not been well investigated. This study investigated some biochemical parameters in the liver and kidney of alloxan-induced diabetic rats treated with Aloe barbadensis juice extract. Alloxan-induced diabetic rats were administered orally with Aloe barbadensis juice extract for seven days after which some biochemical indices in the serum, liver and kidney were measured and compared with the control. Serum alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea and creatinine of untreated diabetic group and treated diabetic groups were significantly elevated when compared with the normal control group with no significant changes in the levels of the enzymes in the liver and kidney. There were no significant changes (p>0.05) in the serum levels of Na+ and K+ in untreated diabetic group and treated diabetic groups when compared with the normal control rats which are not diabetic. These results suggest that administration of aqueous extract of Aloe barbadensis to diabetic rats did not have any adverse effect on the liver and kidney functions in rats showing that the extract is not toxic to man.

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Section: Discussionmentioning

confidence: 99%

Kidney and Liver Function Parameters in Alloxan-Induced Diabetic Rats Treated with Aloe Barbadensis Juice Extract

A¹,

Oluwole²,

Babara³

2010

Sierra Leone j. biomed. res.

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“…The heart was then excised, weighed and homogenised in sucrose buffer solution according to (10,12). The homogenate was frozen overnight to allow proper release of enzymes as indicated by (13). Activities of ALP, LDH and GDH in the supernatant fluid were determined spectrophotometrically as follows: ALP activity was measured by the method of (14) where p-nitrophenyl phosphate was hydrolysed and the absorbance read at 400nm; LDH activity was estimated using the procedure of (15) by measuring the amount ofpyruvate formed from lactate at 440ran and GDH activity by the method of (16) in which conversion of NADH to NAD + was followed at 340nm.…”

Section: Methodsmentioning

confidence: 99%

Changes in the activities of three diagnostic enzymes in the heart of rats following the consumption of diets deficient in zinc and essential fatty acids

Adisa

Odutuga

1998

IUBMB Life

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SummaryChanges in the activities of alkaline phosphatase (ALP), lactate dehydrogenase(LDH) and glutamate dehydrogenase (GDH) in the heart following the consumption of diets deficient in zinc and essential fatty acids (EFA) by rats were investigated. The study was a 2 X 2 factorial design in which rats were fed diets deficient in zinc (5gg/g) or EFA (4% hydrogenated coconut oil) or both. The control diet was adequate in both zinc (100~tg/g) and EFA (4% soyabean oil). The experimental diets were fed for forty-two days after which time the activities of the enzymes were terminally determined in the heart of the rats. The result showed that the activity of ALP was markedly elevated in rats consuming either zinc or EFA deficient diet and slightly in the group deficient in both EFA and zinc. LDH and GDH activities were significantly depressed by EFA only. An interactive effect of zinc and EFA was indicated only for ALP activity. The data indicate a resistance of cardiac tissue to moderate zinc deficiency. However, the effects of EFA were apparent.

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“…In the present report, we have decided to provide information on the mode of cellular toxicity of potassium bromate by assaying for the activities of some carefully selected 'marker' enzymes (alkaline and acid phosphatases, lactate and glutamate dehydrogenases) of cellular damage in rats, since it has been shown that the site of injury to the cell could be correlated and determined by assaying the level of activities of 'marker' enzyme in such cells (Ngaha, 1981). The levels of these enzymes were also assayed in the serum to correlate the changes in the tissues to that of the serum (Ngaha, 1982;Akanji and Yakubu, 2000) and these were complemented with histological examination of the selected tissues.…”

Section: Introductionmentioning

confidence: 99%

Enzyme activities and histopathology of selected tissues in rats treated with potassium bromate.

Akanji¹,

Nafiu²,

Yakubu³

2010

African Journal of Biomedical Research

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The effect of chronic administration of potassium bromate (KBrO 3 ), a flour improver, on some 'marker' enzymes of rat cellular system was investigated. The levels of these enzymes were measured progressively in the kidney, liver and small intestine, 24h after days 1, 3, 5, 10, 15 and 20 following the administration of 10mg/kg body weight of potassium bromate and when left for 10days without administration after their 20 daily doses. The alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and glutamate dehydrogenase (GDH) activities of all the tissues were significantly (P<0.05) decreased in KBrO 3 treated groups with corresponding significant (P<0.05) increase in the serum enzymes. Acid phosphatase (ACP) activities of all the tissues as well as the serum enzyme activities were significantly (P<0.05) decreased. There was only recovery in the ACP activities of all the tissues. Histological examination revealed congestion of the central vein with blood cells in the hepatocytes, infiltration of the interstitial cells accompanied with acute nephritis in the nephrons and mild mucosal dysfunction in the small intestine. The alterations in the 'marker' enzymes as well as in the tissue histology are indications of adverse effect of potassium bromate on the cells which might be due to the presence of oxygen in the molecule leading to oxidation of polyunsaturated fatty acids on the ordered lipid bilayer of cell membranes. All these might be responsible for the various adverse effects associated with the chemical compound.

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Renal effects of potassium dichromate in the rat: Comparison of urinary enzyme excretion with corresponding tissue patterns

Cited by 17 publications

References 8 publications

Kidney and Liver Function Parameters in Alloxan-Induced Diabetic Rats Treated with Aloe Barbadensis Juice Extract

Kidney and Liver Function Parameters in Alloxan-Induced Diabetic Rats Treated with Aloe Barbadensis Juice Extract

Changes in the activities of three diagnostic enzymes in the heart of rats following the consumption of diets deficient in zinc and essential fatty acids

Enzyme activities and histopathology of selected tissues in rats treated with potassium bromate.

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