2012
DOI: 10.1038/modpathol.2012.95
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Reovirus-associated reduction of microRNA-let-7d is related to the increased apoptotic death of cancer cells in clinical samples

Abstract: We analyzed the in situ molecular correlates of infection from cancer patients treated with reovirus. Melanoma, colorectal, and ovarian cancer samples from such patients showed variable infection of the cancer cells but not the intermingled benign cells. RT in situ PCR showed most cancer cells contained the viral genome with threefold less having productive viral infection as documented by either tubulin or reoviral protein co-expression. Productive infection in the cancer cells was strongly correlated with co… Show more

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Cited by 48 publications
(45 citation statements)
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“…19 We grew SiHa (1 HPV DNA copy/cell), HeLa (20 HPV DNA copies/cell), and Caski (hundreds of HPV DNA copies/cell) in DMEM media until 75% confluent, then fixed the cells in 10% buffered formalin overnight, washed the cells several times, then processed them for immunohistochemistry and in situ hybridization analyses.…”
Section: Cell Culturementioning
confidence: 99%
See 3 more Smart Citations
“…19 We grew SiHa (1 HPV DNA copy/cell), HeLa (20 HPV DNA copies/cell), and Caski (hundreds of HPV DNA copies/cell) in DMEM media until 75% confluent, then fixed the cells in 10% buffered formalin overnight, washed the cells several times, then processed them for immunohistochemistry and in situ hybridization analyses.…”
Section: Cell Culturementioning
confidence: 99%
“…18,19 In brief, after using a standard optimization protocol, that included positive controls known to have the target of interest, we tested each tissue for CD8 (Ventana Medical System), PD L1 and PD1 (each from ABCAM). Selected cases that showed strong PD L1 expression and that had sufficient remaining tissue were also tested for CD3, CD68, CD45, CD 117, Ki67, CD45RO, and CD20 (each from Ventana Medical System) as well as RANTES and granyzme B (Enzo Life Sciences).…”
Section: Immunohistochemistrymentioning
confidence: 99%
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“…The viral capsid proteins, including the viral adhesin molecules s1 and s1s, trigger apoptotic pathways characterized by activation of initiator caspases-8 and -9 and release of cytochrome c and Smac/ DIABLO into the cytosol (29). Efforts to elucidate the exact signaling networks that culminate in tumor cell apoptosis are ongoing (30,31). The immunomodulatory impact of reovirus infection is gaining prominence as a mechanism of oncolysis (32).…”
Section: Mechanism Of Tumor Lysismentioning
confidence: 99%