Repair of abdominal wall hernias using acellular dermal matrix in goats

Kumar, Vineet; Kumar, Naveen; Mathew, Dayamon David; Gangwar, Anil Kumar; Saxena, Abhishek; Remya, V.

doi:10.1080/09712119.2012.738222

Cited by 16 publications

(11 citation statements)

References 15 publications

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“…These results were in accordance with Kumar et al . (2012, 2013a,b,c). Recurrence of the hernia is a relatively common complication of herniorrhaphy and has been reported after repair using sutures alone (Cook et al .…”

Section: Discussionmentioning

confidence: 99%

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An acellular aortic matrix of buffalo origin crosslinked with 1‐ethyl‐3‐3‐dimethylaminopropylcarbodiimide hydrochloride for the repair of inguinal hernia in horses

Kumar

Singh

et al. 2013

Equine Veterinary Education

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An acellular aortic matrix (AAM) crosslinked with 1-ethyl-3-3-dimethylaminopropylcarbodiimide hydrochloride (EDC) was evaluated for the repair of inguinal hernias in 5 horses. The aorta from buffalo was acellularised using 1% sodium dodecyl sulfate (SDS) and 0.25% trypsin. The AAM was crosslinked with 1% EDC. Under anaesthesia, inguinal hernias were repaired with EDC-crosslinked AAM graft using an inlay graft technique. Blood samples collected on Days 0, 15 and 30 post implantation, were used for SDS polyacrylamide gel electrophoresis (PAGE) analysis to assess the animals' serum protein concentration, and gelatin zymography for the identification of matrix metalloproteinases. All animals that underwent hernia repair demonstrated successful healing without clinical signs of wound dehiscence, infection or recurrence during a 6-month follow-up period. SDS-PAGE analysis of serum protein concentrations revealed that, this was increased at Day 15 and had decreased again at Day 30. Gelatin zymography of serum of implanted horses expressed a band of 92 kDa, corresponding to MMP-9 activity. The relative amount of the 92 kDa band was higher at Day 15 as compared to Days 0 and 30. It may be concluded that EDC-crosslinked AAM of buffalo origin can be used safely in horses for the repair of inguinal hernia with adequate strength and minimal foreign body reaction.

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Section: Discussionmentioning

confidence: 99%

“…2012; Kumar et al . 2013a,b,c). The potential benefits of biological materials include improved infection resistance, host tissue ingrowth and less adhesion formation (Gaertner et al .…”

Section: Introductionmentioning

confidence: 99%

An acellular aortic matrix of buffalo origin crosslinked with 1‐ethyl‐3‐3‐dimethylaminopropylcarbodiimide hydrochloride for the repair of inguinal hernia in horses

Kumar

Singh

et al. 2013

Equine Veterinary Education

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“…The extracellular matrix (ECM) scaffolds primarily composed of structural collagen protein are widely used in tissue engineering and regenerative medicine [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15]. These are usually prepared from animal tissues by decellularization process.…”

Section: Introductionmentioning

confidence: 99%

“…Enzymatic techniques of decellularization include the use of protease (trypsin) [1-5, 8, 10, 12-15], endonucleases and exonucleases. Chemical methods of decellularization include the use of acids and alkalis (acetic acid, peracetic acid, hydrochloric acid, sulfuric acid, ammonium hydroxide), nonionic detergents (Triton X-100), ionic detergents (sodium dodecyl sulfate, sodium deoxycholate, Triton X-200) [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15], zwitterionic detergents (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, sulfobetaine-10, sulfobetaine-16), organic solvent (Tri(n-butyl)phosphate) [3,10], hypertonic and hypotonic solutions [2,3,8,10,13,15], and chelating agents (EDTA). These reagents at higher concentrations extensively disrupt the structural proteins of ECM scaffolds and make it impossible to analyze by routine techniques [17].…”

Section: Introductionmentioning

confidence: 99%

Fourier Transform Infrared Spectroscopy of the Animal Tissues

Kumar¹,

Vora²,

Asodiya³

et al. 2021

Real Perspective of Fourier Transforms and Current Developments in Superconductivity

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Animal tissues are extensively used as scaffolds for tissue engineering and regenerative therapies. They are typically subjected to decellularization process to obtain a cell-free extracellular matrix (ECM) scaffolds. It is important to identify chemical structure of the ECM scaffolds and Fourier transform infrared (FTIR) appears to be a technique of choice. In this chapter, FTIR spectra of native and decellularized buffalo aortae, buffalo diaphragms, goat skin, and native bovine cortical bone are presented. The transmittance peaks are that of organic collagen amide A, amide B, amide I, amide II and amide III chemical functional groups in both native and decellularized aortae, diaphragms and skin. In bone, the transmittance peaks are that of inorganic ν1, ν3 PO43−, OH− in addition to organic collagen amide A, amide B, amide I, amide II and amide III chemical functional groups. These important transmittance peaks of the tissue samples will help researchers in defining the chemical structure of these animal tissues.

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“…[21][22][23] Several studies reported that ADM grafts have been used for the reconstruction of abdominal wall defects in rabbits, rats, goats, and humans. [24][25][26][27] Although the results of the studies were promising, the personalized reconstruction of the ADM as needed is limited, and the cost is very high. In addition, to our knowledge, there is no research on using ADM powder to reconstruct abdominal wall defects.…”

Section: Introductionmentioning

confidence: 99%

A facile strategy for fabricating composite patch scaffold by using porcine acellular dermal matrix and gelatin for the reconstruction of abdominal wall defects

Liu

Zhang

et al. 2020

J Biomater Appl

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Defects of the abdominal wall continue to be a significant issue in regenerative medicine and tissue engineering. Porcine acellular dermal matrix (P-ADM) and gelatin have been widely used for fabricating engineered abdominal wall due to their good biocompatibility and integration with host tissues. In this study, we demonstrate a novel and reproducible approach of using gelatin and P-ADM powder to fabricate composite patch scaffolds by combining solvent casting and enzymatic cross-linking for the abdominal wall regeneration in rats. Different concentration ratios of gelatin and P-ADM and different shapes of engineered scaffolds are studied for various abdominal wall defects. Skeletal muscle cells and human umbilical vein endothelial cells can successfully attach to the surface of the scaffolds and maintain high viability after culturing for five days. In vivo experiments demonstrate that there are no signs of infection, intra-abdominal adhesion, and severe inflammation after two weeks, which indicate good repairability of the scaffolds. In conclusion, we propose a novel method that holds great potential for building engineered scaffolds to meet the different requirements of abdominal wall regeneration.

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Repair of abdominal wall hernias using acellular dermal matrix in goats

Cited by 16 publications

References 15 publications

An acellular aortic matrix of buffalo origin crosslinked with 1‐ethyl‐3‐3‐dimethylaminopropylcarbodiimide hydrochloride for the repair of inguinal hernia in horses

An acellular aortic matrix of buffalo origin crosslinked with 1‐ethyl‐3‐3‐dimethylaminopropylcarbodiimide hydrochloride for the repair of inguinal hernia in horses

Fourier Transform Infrared Spectroscopy of the Animal Tissues

A facile strategy for fabricating composite patch scaffold by using porcine acellular dermal matrix and gelatin for the reconstruction of abdominal wall defects

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