Repeatability of the efficiency of columns packed with sub-3μm core–shell particles: Part I. 2.6μm Kinetex-C18 particles in 4.6mm and 2.1mm×100mm column formats

Gritti, Fabrice; Guiochon, Georges

doi:10.1016/j.chroma.2012.05.072

Cited by 49 publications

(11 citation statements)

References 56 publications

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“…Differences in column efficiency between columns were attributed merely to the random nature of the packing process and the resulting lack of homogeneity of the column bed . For 2.6 μm SPPs in 2.1 × 100 mm column formats, the RSD of the eddy diffusion contribution was <10% . Similar results were found for SPPs with larger pore size designed for the separation of peptides .…”

Section: Core–shell Particles As Packing Materials and The Mechanism supporting

confidence: 58%

Core–shell microspheres with porous nanostructured shells for liquid chromatography

Ahmed

Skinley

Herodotou

et al. 2017

J of Separation Science

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The development of new stationary phases has been the key aspect for fast and efficient high-performance liquid chromatography separation with relatively low backpressure.Core-shell particles, with a solid core and porous shell, have been extensively investigated and commercially manufactured in the last decade. The excellent performance of core-shell particles columns has been recorded for a wide range of analytes, covering small and large molecules, neutral and ionic (acidic and basic), biomolecules and metabolites. In this review, we first introduce the advance and advantages of coreshell particles (or more widely known as superficially porous particles) against nonporous particles and fully porous particles. This is followed by the detailed description of various methods used to fabricate core-shell particles. We then discuss the applications of common silica core-shell particles (mostly commercially manufactured), spheres-on-sphere particles and core-shell particles with a non-silica shell. This review concludes with a summary and perspective on the development of stationary phase materials for high-performance liquid chromatography applications.

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Section: Core–shell Particles As Packing Materials and The Mechanism supporting

confidence: 58%

Core–shell microspheres with porous nanostructured shells for liquid chromatography

Ahmed

Skinley

Herodotou

et al. 2017

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Differences in column efficiency between columns were attributed merely to the random nature of the packing process and the resulting lack of homogeneity of the column bed 51 . For 2.6 m commercially available shell particles in 2.1 x 100mm column formats, the rsd of the eddy diffusion contribution was less than 10% 52 . Similar results were found for shell particles with larger pore size designed for the separation of peptides 53 .…”

Section: Reasons For the High Efficiency Of Shell Particlesmentioning

confidence: 89%

Core–Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography

Tanaka¹,

McCalley

2015

Anal. Chem.

154

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“…Similar results have been observed with larger-bore (i.e., larger inner diameter) columns [29], but it was not given that this would be the case for nano-scale columns, as successful packing/polymerization can be dependent on column diameter. For example, solid core particles packed in 2.1 mm ID columns have previously not been able to match the efficiency of comparable 4.6 mm ID columns [30], while monolith columns are easier to prepare in capillary/nanoformat [31]. …”

Section: Discussionmentioning

confidence: 99%

Comparison of Commercial Nanoliquid Chromatography Columns for Fast, Targeted Mass Spectrometry-Based Proteomics

et al. 2016

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Aim:We compared four commonly used, commercially available reverse phase nanoLC columns for identification/determination of Wnt/β-catenin-related pathway proteins.Materials & methods:The columns were: Chromolith® (silica monolith; Merke Millipore, MA, USA), PepMap™ (porous particles; Thermo Fisher Scientific, MA, USA), Accucore™ (solid core particles; Thermo Fisher Scientific) and PepSwift™ (organic monolith; Thermo Fisher Scientific).Results:The peak capacity of the columns varied from 100 (Pepswift) to 190 (Accucore) (for 30 min gradients). All columns enabled identification/detection of GSK3β and β-catenin in the complex samples. However, even the columns with higher peak capacities could not enable detection of the somewhat less abundant proteins AXIN2 and TNKS2. The monoliths were more prone to retention time instability when sample complexity increased.Conclusion:We find that commercial nanoLC columns, although featuring different morphologies and peak capacities, provided surprisingly few practical differences for relatively fast, targeted determination of proteins.

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Repeatability of the efficiency of columns packed with sub-3μm core–shell particles: Part I. 2.6μm Kinetex-C18 particles in 4.6mm and 2.1mm×100mm column formats

Cited by 49 publications

References 56 publications

Core–shell microspheres with porous nanostructured shells for liquid chromatography

Core–shell microspheres with porous nanostructured shells for liquid chromatography

Core–Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography

Comparison of Commercial Nanoliquid Chromatography Columns for Fast, Targeted Mass Spectrometry-Based Proteomics

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