Repeated Doses of UVR Cause Minor Alteration in CytokineSerum Levels in Humans

Abstract

The aim of our study was to compare serum concentration of IL-1β, IL-6, IL-8, IL-10, and TNF-α in 105 healthy volunteers before and after exposure to UVR: 25 subjects (10 days of UVB), 55 (10 days of UVB or solar-simulated radiation, followed by acute UVB dose), and 25 (local high dose of UVB). In all the individuals blood samples were analyzed before and after final irradiation by chemiluminescence assay. After 10 days of UVB irradiation a statistically significant increase in serum concentration only in IL-8 (P < .05) and strong tendency in TNF-α (P = .05) were observed. The applied schedules of irradiation have minor impact on serum cytokine level and still a threshold dose of UVR causing systemic immune impairment is unknown.

Differential modulation of inflammatory markers in plasma and skin after single exposures to UVA or UVB radiation in vivo

“…In contrast, no significant changes in TNF-α and IL-1β level were found in plasma of human subjects at 24 h following a single exposure to 3 MED of UVB (280-315 nm). Repeated UVB irradiation (10 x 0.7 MED) induced a statistically significant increase only in plasma TNF-α level; no effect was found on IL-1β levels 18 . Repeated SSL (280-400 nm; 96% UVA) exposures (10 x 120 J/m 2 ) caused a significant increase in mRNA levels of IL-1β and TNF-α in human skin 19 , but no changes in plasma level of these cytokines 24 h after the last exposure 18 .…”

“…Repeated UVB irradiation (10 x 0.7 MED) induced a statistically significant increase only in plasma TNF-α level; no effect was found on IL-1β levels 18 . Repeated SSL (280-400 nm; 96% UVA) exposures (10 x 120 J/m 2 ) caused a significant increase in mRNA levels of IL-1β and TNF-α in human skin 19 , but no changes in plasma level of these cytokines 24 h after the last exposure 18 . Three whole-body exposures of human subjects to UVA (340-400 nm) or UVB (311-313 nm) did not affect TNF-α and IL-1β serum level at several time points (0-48 h) (ref.…”

“…In UVA irradiated mice, we found a significant IL-10 increase in both plasma and skin samples after 24 h. Shen et al reported that UVA (37.8 J/cm 2 ) irradiation caused non-significant but detectable increase in IL-10 level in mice skin during 0-120 h after exposure 25 . On the other hand, repeated SSL (280-400 nm; 96% UVA) exposures (10 x 120 J/m 2 ) of human volunteers caused a significant increase in mRNA levels of IL-10 in skin 19 , but no changes in plasma protein level of the cytokine 24 h after the last exposure 18 . The results suggest that UVB-caused IL-10 increase is probably linked to enhanced leukocyte infiltration and DNA damage (thymine dimmer formation) (ref.…”

“…Local exposure of human volunteers to SSL (1 and 3 MED) induced a strong increase in IL-10 mRNA level 24 h after exposure 13 . Narbutt et al found enhanced IL-10 mRNA level in skin 17 but unchanged protein level in plasma 18 at 24 h after a single exposure to 3 MED of UVB (280-315 nm) exposure. In UVA irradiated mice, we found a significant IL-10 increase in both plasma and skin samples after 24 h. Shen et al reported that UVA (37.8 J/cm 2 ) irradiation caused non-significant but detectable increase in IL-10 level in mice skin during 0-120 h after exposure 25 .…”

“…In human subjects, Urbanski et al found the peak IL-6 concentration in plasma at 12 h after following the treatment with a UV source emitting light of 250-600 nm (emission peaks 300 and 360 nm) (ref. level in plasma of human volunteers at 24 h following a single (3 MED) and repeated (10 x 0.7 MED) UVB (280-315 nm) exposure 18 . In contrast, massive increase in IL-6 mRNA level was found in human skin 24 h after a single exposure to 3 MED of UVB (280-315 nm) (ref.…”