Replacement of fetal calf serum by human serum as supplementation for human fibroblast culture

Isaac, César; Mattos, Cristiana Nicoli de; Rego, Francinni M. P.; Cardim, Larissa Nocchi; Altran, Silvana Cereijido; Paggiaro, André Oliveira; Tutihashi, Rafael Mamoru Carneiro; Mathor, Mônica Beatriz; Ferreira, Marcus Castro

doi:10.1590/s1983-51752011000300003

Cited by 3 publications

(7 citation statements)

References 24 publications

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“…A significant amount of fibroblast cells was stained by vimentin, while the blood cells trapped in PRF during the centrifugation were not stained by vimentin, showing the possibility of PPP 10% in maintaining the viability of those human cells. The PPP provides a significant amount of platelet-derived growth factor (PDGF-AB), transforming growth factor-β1(TGF-β1) and other bioactive molecules ( 13 ) ( 9 , 10 ) and this can explain the results that showed that 10% PPP provided similar cell proliferation when compared to control group.…”

Section: Discussionmentioning

confidence: 99%

“…Besides, xenogeneic agents could pose risks to patients’health due to their ability to generate antibodies against factor V and XI, which could cause coagulopathies ( 11 , 16 ). The PRF is rich in growth factors, platelets and fibrin,thus having high potential for clinical application ( 9 , 10 ). However, the study of PPP, which isobtained of PRF, has been poorly investigated even though ithas a high range of important biomolecules ( 13 , 14 ).…”

Section: Discussionmentioning

confidence: 99%

“…Ranges of culture media, including Dulbecco’s Modified Eagle Medium (DMEM), have been employed for the in vitro maintenance of MC. To allow the cells remaining in physiological conditions, the culture medium should be supplemented with a complex mixture of growth factors, proteins, carbohydrates and cytokines ( 9 )[REMOVED HYPERLINK FIELD]. Fetal Bovine Serum (FBS) is the solution commonly applied for this purpose, despite present risks associated with transmission of zoonosis and pathogens ( 7 ).…”

Section: Introductionmentioning

confidence: 99%

“…Besides, animal (xenogeneic) proteins that are present in FBS can be internalized into the human cells byactivatingantigenic response post-implantation. In addition, FBS-proteins remain internalized incells cytoplasm evenafter successive washings ( 9 ) andmay causechanges in the surface markers, which couldinducealterationsin cells biology ( 7 ).…”

Section: Introductionmentioning

confidence: 99%

“…Venous blood derivatives (VBD)have been developed as an alternative to traditional supplements to overcome the risks related to xenogeneic serums. Venous blood derivatives such as Platelet Rich Plasma have been widely studied, proving to be able to maintainMS culture as well as FBS ( 9 , 10 ).The use of platelet concentrates in tissue regeneration can provide a more effective healing and can accelerate the repair process ( 11 ). However, the protocols for obtaining the PRP are complex and there is a need for incorporation of chemicals,which are indispensable for proper PRP processing ( 12 ).…”

Section: Introductionmentioning

confidence: 99%

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Platelet-Poor Plasma as a Supplement for Fibroblasts Cultured in Platelet-Rich Fibrin

Chisini¹,

Karam²,

Noronha³

et al. 2017

Acta Stomatol Croat

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The aim of this study was to evaluate the proliferation and adhesion of mesenchymal cells (3T3/NIH) in Dulbecco’s Modified Eagle Medium(DMEM) supplemented with Platelet-Poor Plasma (PPP) in aPlatelet-Rich Fibrin (PRF) scaffold. Human blood was obtained and processed in a centrifuge considering the equation G=1.12xRx(RPM/1000)2 to obtain PRF and PPP.Cell adhesion and maintenance analyses were performed by MTTassays in a 96 well plate withsupplemented DMEM: PPP (90:10) for 24 hours. Besides, the PRF was deposited in a 48 well plate and 10x104 cells were seeded above each PRF (n=3) with 800µl of DMEM: PPP (90:10) and cultured for 7 days. Histological analysis and the immunohistochemical staining for Vimentin were performed. Results were analyzed by one-way ANOVA in Stata12®. A significant decrease (p<0.05) of cells adhesion in relationship to FBSwas observed. However, a similar ability of cell-maintenance for PPP 10% was observed (P>0.05). Fibroblasts culture for 7 days in PRF supplemented with PPP 10% was possible, showing positive staining for Vimentin. Therefore, PPP cell supplementation decreased the initial adhesion of cells but was able to maintain the proliferation of adhered cells and able to support their viability in PRF.It seems that this method has many clinical advantagessince it provides an autologous and natural scaffold with their respective supplement for cell culture by only one process, without using xenogeneic compounds. This could improve the potential of clinical translational therapies based on the use of PRF cultured cells, promoting the regenerative potential for future use in medicine and dentistry.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Platelet-Poor Plasma as a Supplement for Fibroblasts Cultured in Platelet-Rich Fibrin

Chisini¹,

Karam²,

Noronha³

et al. 2017

Acta Stomatol Croat

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Steroid-depleted polycystic ovarian syndrome serum promotesin vitrooocyte maturation and embryo development

Eyvaznejad

Nouri

Ghasemzadeh

et al. 2018

Gynecological Endocrinology

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In vitro maturation (IVM) of immature oocytes obtained from patients with polycystic ovarian syndrome (PCOS) is considered as a novel strategy in order to reduce clinical side effects and cost of in vitro fertilization (IVF) technique. The aim of this study was to evaluate the effects of PCOS whole and steroid-depleted serums on in vitro oocyte maturation indices. Patients with PCOS were selected according to the Rotterdam criteria. Cumulus-oocyte complexes and blood serums were collected and pooled. Cumulus cells and immature oocytes were treated with 10% whole or steroid-depleted serums. Stearoyl-CoA desaturase-1 (SCD1) and cyclooxygenase-2 (COX2) expression levels in cumulus cells were evaluated by quantitative PCR. Fatty acid composition of cumulus cells was analyzed using gas-liquid chromatography. Polar body observation was considered as the oocyte maturation index. Oleate (1.28-fold, p = .006), SCD1 expression (450-fold, p = .001), and COX2 expression (35-fold, p = .02) in cumulus cell, as well as oocyte maturation (p < .001) and in vitro embryo development (p < .05) were significantly higher in treatment with steroid-depleted serum compared to that of whole serum. Steroid depletion of PCOS serum improved its capacity to increase success rate of oocyte maturation, intra-cytoplasmic sperm injection and early embryo development.

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Behaviour of Titanium Dioxide Particles in Artificial Body Fluids and Human Blood Plasma

Korábková

Kašpárková

Jasenská

et al. 2021

IJMS

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The growing application of materials containing TiO2 particles has led to an increased risk of human exposure, while a gap in knowledge about the possible adverse effects of TiO2 still exists. In this work, TiO2 particles of rutile, anatase, and their commercial mixture were exposed to various environments, including simulated gastric fluids and human blood plasma (both representing in vivo conditions), and media used in in vitro experiments. Simulated body fluids of different compositions, ionic strengths, and pH were used, and the impact of the absence or presence of chosen enzymes was investigated. The physicochemical properties and agglomeration of TiO2 in these media were determined. The time dependent agglomeration of TiO2 related to the type of TiO2, and mainly to the type and composition of the environment that was observed. The presence of enzymes either prevented or promoted TiO2 agglomeration. TiO2 was also observed to exhibit concentration-dependent cytotoxicity. This knowledge about TiO2 behavior in all the abovementioned environments is critical when TiO2 safety is considered, especially with respect to the significant impact of the presence of proteins and size-related cytotoxicity.

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Replacement of fetal calf serum by human serum as supplementation for human fibroblast culture

Cited by 3 publications

References 24 publications

Platelet-Poor Plasma as a Supplement for Fibroblasts Cultured in Platelet-Rich Fibrin

Platelet-Poor Plasma as a Supplement for Fibroblasts Cultured in Platelet-Rich Fibrin

Steroid-depleted polycystic ovarian syndrome serum promotesin vitrooocyte maturation and embryo development

Behaviour of Titanium Dioxide Particles in Artificial Body Fluids and Human Blood Plasma

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