Replacement Process of Carbonate Apatite by Alveolar Bone in a Rat Extraction Socket

Zhang, Xiaoxu; Atsuta, Ikiru; Narimatsu, Ikue; Ueda, Nobuyuki; Takahashi, Ryuji; Egashira, Yuki; Zhang, Jing Qi; Gu, Jian; Koyano, Kiyoshi; Ayukawa, Yasunori

doi:10.3390/ma14164457

Cited by 11 publications

(14 citation statements)

References 42 publications

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“…To surpass the efficacy of previous HC scaffolds and satisfy these requirements, an effective approach is to control the chemical composition and pore structure of the HC scaffold, which can crucially affect its osteoconductivity and resorption. In terms of the chemical composition, carbonate apatite (CAp) is resorbed by the osteoclasts at a similar pace as that of new bone formation and can thus be replaced with new bone (Doi et al, 1998;Zhang et al, 2021). In contrast, hydroxyapatite (HAp) is not resorbed and beta-tricalcium phosphate (β-TCP) is resorbed prior to new bone formation (Ishikawa et al, 2018;Hayashi et al, 2019bHayashi et al, , 2020b.…”

Section: Introductionmentioning

confidence: 99%

Effects of Channels and Micropores in Honeycomb Scaffolds on the Reconstruction of Segmental Bone Defects

Shibahara

Hayashi

Nakashima

et al. 2022

Front. Bioeng. Biotechnol.

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The reconstruction of critical-sized segmental bone defects is a key challenge in orthopedics because of its intractability despite technological advancements. To overcome this challenge, scaffolds that promote rapid bone ingrowth and subsequent bone replacement are necessary. In this study, we fabricated three types of carbonate apatite honeycomb (HC) scaffolds with uniaxial channels bridging the stumps of a host bone. These HC scaffolds possessed different channel and micropore volumes. The HC scaffolds were implanted into the defects of rabbit ulnar shafts to evaluate the effects of channels and micropores on bone reconstruction. Four weeks postoperatively, the HC scaffolds with a larger channel volume promoted bone ingrowth compared to that with a larger micropore volume. In contrast, 12 weeks postoperatively, the HC scaffolds with a larger volume of the micropores rather than the channels promoted the scaffold resorption by osteoclasts and bone formation. Thus, the channels affected bone ingrowth in the early stage, and micropores affected scaffold resorption and bone formation in the middle stage. Furthermore, 12 weeks postoperatively, the HC scaffolds with large volumes of both channels and micropores formed a significantly larger amount of new bone than that attained using HC scaffolds with either large volume of channels or micropores, thereby bridging the host bone stumps. The findings of this study provide guidance for designing the pore structure of scaffolds.

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Section: Introductionmentioning

confidence: 99%

Effects of Channels and Micropores in Honeycomb Scaffolds on the Reconstruction of Segmental Bone Defects

Shibahara

Hayashi

Nakashima

et al. 2022

Front. Bioeng. Biotechnol.

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“…The OECs and FBs morphology on CO 3 Ap or HAp plates (diameter 10.0 mm, thickness 0.8 mm), which was kindly provided by GC (Tokyo, Japan), was evaluated by scanning electron microscopy (SEM) [ 12 ] (Fig. 2 A).…”

Section: Methodsmentioning

confidence: 99%

“…Rats received care following the guidelines established by Kyushu University (approval number: A29-227-0). Extraction and transplant was performed as previously reported [ 12 ]. Briefly, 6-week-old Wistar rats (72 males; 120–150 g) had extraction of the maxillary right first and second molars under systemic anesthesia.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, 6-week-old Wistar rats (72 males; 120–150 g) had extraction of the maxillary right first and second molars under systemic anesthesia. The extraction socket was enlarged with a dental round bar, and the CO 3 Ap (Cytrans Granule, GC; particle size 0.3–0.6 mm) or HAp bone substitute (Bonetite granule perio, HOYA Technosurgical, Tokyo Japan; particle size 0.3–1.0 mm) was implanted [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

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Effect of carbonate apatite as a bone substitute on oral mucosal healing in a rat extraction socket: in vitro and in vivo analyses using carbonate apatite

et al. 2022

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Background Low bone quantity and quality are serious problems that affect the prognosis of implants in the cosmetic field. Therefore, artificial bone substitutes are frequently used. However, whether there is a difference in the effect of either bone substitute on soft tissue healing is unclear given their greatly different absorbability. In this study, we used hydroxyapatite (HAp) and carbonate apatite (CO3Ap) as bone substitutes to analyze the epithelial and connective tissue healing after tooth extraction. Methods In vitro, oral mucosa-derived epithelial cells (OECs) collected from 4-day-old Wistar rats were seeded on HAp or CO3Ap and evaluated for adhesion, proliferation, migration, apoptosis, and morphology. Fibroblasts (FBs) were also analyzed for their ability to express collagen. In vivo, the extraction of maxillary right first (M1) and second molars (M2) of 6-week-old male Wistar rats was performed, followed by insertion of HAp or CO3Ap granules into the M1 and M2 sites. The oral mucosal healing process was then evaluated histochemically after 7 and 14 days. Results In vitro, high collagen expression by FBs in the CO3Ap group was observed and the surface analysis showed spreading of the FBs on the CO3Ap surface. However, the activity of OECs was suppressed on CO3Ap. Two weeks after CO3Ap implantation, soft tissue healing was observed, and recovery of the connective tissue was observed on the remaining CO3Ap. Conclusions Our results suggest that the formation of soft tissues, including connective tissue, was promoted by CO3Ap in the extraction socket within a short period.

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“…CAP promotes osteoblast differentiation and CAP artificial bones have high bone conductivity [ 12 , 13 ]. A comparison between CAP bone prostheses and autologous bone revealed that CAP was similarly absorbed and replaced by bone in osteoclast precursor cell-culture experiments and in a rat tooth extraction socket model [ 14 ]. In addition, CAP bone prostheses showed promising results in the biopsy–histological assessment upon implant entry and three years after implantation in two-way cases of maxillary sinus floor elevation [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Combination of Carbonate Hydroxyapatite and Stem Cells from Human Deciduous Teeth Promotes Bone Regeneration by Enhancing BMP-2, VEGF and CD31 Expression in Immunodeficient Mice

Putranti

Kunimatsu

Rikitake

et al. 2022

Cells

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The objective of this study was to clarify the efficiency of a combination of stem cells from human deciduous teeth and carbonate apatite in bone regeneration of calvarial defects. Immunodeficient mice (n = 5 for each group/4 groups) with artificial calvarial bone defects (5 mm in diameter) were developed, and stem cells from human deciduous teeth (SHEDs) and carbonate hydroxyapatite (CAP) granules were transplanted with an atelocollagen sponge as a scaffold. A 3D analysis using microcomputed tomography, and 12 weeks after transplantation, histological and immunohistochemical evaluations of markers of bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF), and cluster of differentiation (CD) 31 were performed. In the 3D analysis, regenerated bone formation was observed in SHEDs and CAP, with the combination of SHEDs and CAP showing significantly greater bone regeneration than that in the other groups. Histological and immunohistochemical evaluations showed that combining SHEDs and CAP enhanced the expression of BMP-2, VEGF, and CD31, and promoted bone regeneration. This study demonstrates that the combination of SHEDs and CAP transplantation may be a promising tool for bone regeneration in alveolar defects.

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Replacement Process of Carbonate Apatite by Alveolar Bone in a Rat Extraction Socket

Cited by 11 publications

References 42 publications

Effects of Channels and Micropores in Honeycomb Scaffolds on the Reconstruction of Segmental Bone Defects

Effects of Channels and Micropores in Honeycomb Scaffolds on the Reconstruction of Segmental Bone Defects

Effect of carbonate apatite as a bone substitute on oral mucosal healing in a rat extraction socket: in vitro and in vivo analyses using carbonate apatite

Combination of Carbonate Hydroxyapatite and Stem Cells from Human Deciduous Teeth Promotes Bone Regeneration by Enhancing BMP-2, VEGF and CD31 Expression in Immunodeficient Mice

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