Replication of Oryctes Baculovirus in Cell Culture: Viral Morphogenesis, Infectivity and Protein Synthesis

Crawford, A. M.; Sheehan, Cathy

doi:10.1099/0022-1317-66-3-529

Cited by 45 publications

(39 citation statements)

References 19 publications

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“…This is not surprising as these viruses do not have any form of inclusion body. There is also no late gene expression analogous to that of the polyhedrin/granulin gene (Crawford & Sheehan, 1985). Unfortunately it is these conserved sequences which have been used to orientate the previously published maps (Vlak & Smith, 1982).…”

Section: Discussionmentioning

confidence: 99%

“…O0"ctes baculovirus, strain PV505, was cloned by endpoint dilution as previously described (Crawford & Sheehan, 1985). Oryctes baculovirus was grown in DSIR-HA1179 cells (Crawford, 1982) or adult Oryctes rhinoceros.…”

Section: Methodsmentioning

confidence: 99%

“…With the exception of Oryctes baculovirus and the Hz-I virus found in the IMC-Hz-1 cell line none has been characterized beyond its appearance in thin sections viewed with an electron microscope (Crawford & Granados, 1982). Three major differences have been identified between Oryctes baculovirus and the more intensively studied subgroup A baculoviruses: the absence of unenveloped nucleocapsids during virus morphogenesis in the nucleus, the acquisition of a second unit membrane by those particles budded from the plasma membrane, and the absence of any inclusion body production including late protein synthesis (Crawford & Sheehan, 1985).…”

Section: Introductionmentioning

confidence: 99%

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A Physical Map of the Oryctes Baculovirus Genome

Crawford¹,

Ashbridge

Sheehan³

et al. 1985

Journal of General Virology

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SUMMARYThe restriction endonucleases, BamHI, EcoRl, HindlII and PstI, cleave Oryctes baculovirus (strain PV505) DNA into 21, 43, 23 and seven fragments respectively. A large number of these fragments were cloned into the bacterial plasmids pUC8 and pBR328. These clones encompass 96 % of the genome. The restriction sites for the four endonucleases were mapped using double and partial digestions of cloned fragments as well as hybridization of labelled fragments to Southern transfers of cleaved DNA.When HindlII fragment D and BamHI fragment D were hybridized to Southern transfers six regions containing reiterated sequences were found. The physical map for Oryctes baculovirus could not be orientated with respect to other published baculovirus maps because the BamHI fragment F of Autographa californiea nuclear polyhedrosis virus which contains conserved polyhedrin gene sequences common to occluded baculoviruses did not bind to Oryctes baculovirus DNA.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Physical Map of the Oryctes Baculovirus Genome

Crawford¹,

Ashbridge

Sheehan³

et al. 1985

Journal of General Virology

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“…There have been studies on the molecular and structural biology of OrNV (Payne 1974;Richards et al 1999;Mohan and Gopinathan 1989;Crawford and Zelazny 1990), its viral pathogenesis in DSIR-HA-1179 cells (Crawford and Sheehan 1985), and more recent studies on nudivirus genomics (Wang et al 2011). However, no literature currently exists on the characterization of this cell line from a technological perspective, with the exception of an initial estimation of the population doubling time of 6 days shortly after establishment of the DSIR-HA-1179 cell line (Crawford 1982).…”

Section: Introductionmentioning

confidence: 99%

Characterization of growth and Oryctes rhinoceros nudivirus production in attached cultures of the DSIR-HA-1179 coleopteran insect cell line

et al. 2013

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The DSIR-HA-1179 coleopteran cell line is a susceptible and permissive host to the Oryctes rhinoceros nudivirus (OrNV), which has been used as a biocontrol agent against the coconut rhinoceros beetle (Oryctes rhinoceros); a pest of palms in the Asia-Pacific region. However, little is known about growth and metabolism of this cell line, knowledge of which is necessary to develop an in vitro large-scale OrNV production process. The strong anchoragedependent characteristics of the cell line, its particular fragility and its tendency to form dense clumps when manipulated, are the most likely reasons that have precluded further development of the cell line. In order to characterize DSIR-HA-1179 cells, there was first a need for a reliable technique to count the cells. A homogenous cell suspension suitable for enumeration could be produced by treatment with TrypLE Express TM with optimum mean time for cell release calculated as 30 min. The cell line was adapted to grow in four serum-supplemented culture media namely TC-100, IPL-41, Sf-900 II and Sf-900 III and cell growth, glucose consumption, lactate and ammonia production were assessed from static-batch cultures. The maximum viable cell density was reached in Sf-900 II (17.9 9 10 5 cells/ml), with the maximum specific growth rate observed in this culture medium as well (0.0074 h -1 ). Higher production of OrNV was observed in IPL-41 and TC-100 (4.1 9 10 7 TCID 50 /ml) than in cultures infected in Sf-900 III (2.0 9 10 7 TCID 50 /ml) and Sf-900 II (1.4 9 10 7 TCID 50 /ml). At the end of the growth period, glucose was completely consumed in cultures grown in TC-100, while remained in excess in the other three culture media. The cell line produced lactate and ammonia to very low levels in the TC-100 culture medium which is a promising aspect for its cultivation at large-scale.

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“…All virus isolates were twice cloned by endpoint dilution as previously described (Crawford & Sheehan, 1985). Isolates were replicated in DSIR-HA-1179 cells (Crawford, 1982) and the DNA was purified from pelleted, extracellular virus as previously described (Crawford et al, 1985).…”

mentioning

confidence: 99%

Genotypic Variation in Geographical Isolates of Oryctes Baculovirus

Crawford¹,

Zelazny²,

Alfiler³

1986

Journal of General Virology

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SUMMARYTwelve geographical isolates of Oryctes baculovirus were cloned in DSIR-HA-1179 cells. The DNA of these isolates was analysed by restriction endonuclease digestion with the enzymes BamHI, EcoRI, HindIII and PstI. Each isolate showed slightly different restriction fragment electrophoresis profiles. Most of the changes were due to small insertions or deletions of DNA, although two isolates lacked a single restriction site. The sites of genotypic change were not randomly distributed but were mainly associated with regions that have been shown to contain reiterated sequences.

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Replication of Oryctes Baculovirus in Cell Culture: Viral Morphogenesis, Infectivity and Protein Synthesis

Cited by 45 publications

References 19 publications

A Physical Map of the Oryctes Baculovirus Genome

A Physical Map of the Oryctes Baculovirus Genome

Characterization of growth and Oryctes rhinoceros nudivirus production in attached cultures of the DSIR-HA-1179 coleopteran insect cell line

Genotypic Variation in Geographical Isolates of Oryctes Baculovirus

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