Report of ribosomal RNA methylase gene erm(B) in multidrug-resistant Campylobacter coli

Qin, Shangshang; Wang, Yang; Zhang, Qijing; Zhang, Maojun; Deng, Fengru; Shen, Zhangqi; Wu, Congming; Wang, Shaolin; Zhang, Jianzhong; Shen, Jianzhong

doi:10.1093/jac/dkt492

Cited by 104 publications

(126 citation statements)

References 35 publications

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“…The detailed genetic features of the erm(B)-carrying plasmids and their transfer mechanisms remain to be determined in future studies. The identification of multiple erm(B)-positive isolates in this study reinforced the findings in our recent report (11) and indicates that erm(B) has emerged and is disseminating in Campylobacter. This finding is significant, as macrolides are the key antibiotic for clinical therapy of Campylobacter infections and resistance mediated by rRNA methylase was not identified until recently (11).…”

Section: Erm(b) In Campylobactersupporting

confidence: 90%

“…Our previous study showed that the type I MDRGI from ZC113 could be transferred to C. jejuni NCTC 11168 via natural transformation (11). In this study, we examined five other types of MDRGIs and found that they were also transferable from C. coli isolates to C. Table 2).…”

Section: Erm(b) In Campylobactermentioning

confidence: 78%

“…These include 75 human isolates, 789 swine isolates, 433 chicken isolates, 227 duck isolates, and 30 isolates from chicken carcasses. The previously identified erm(B)-carrying C. coli ZC113 of swine origin was included as a reference in this study (11). The 75 human C. coli isolates were from the retrospective collection of Chinese Centers for Disease Control and Prevention (CDC, Beijing, China) and were obtained during 2001-2011 from clinically confirmed gastroenteritis in four different provinces or cities.…”

Section: Methodsmentioning

confidence: 99%

“…rRNA methylases, which represent major mechanisms for macrolide resistance in other bacterial organisms (10), were not identified in Campylobacter until recently. A recent work by our group reported the identification of a Gram-positive erm(B) gene in a single porcine Campylobacter coli isolate (11), which carried no mutations in the 23S rRNA or in the rplD and rplV genes. erm(B) encodes a rRNA methylase and mediates highlevel macrolide resistance (MIC ϭ 512 g/ml) in Campylobacter, and it can be transferred between Campylobacter jejuni and Campylobacter coli via natural transformation (11).…”

mentioning

confidence: 91%

“…A recent work by our group reported the identification of a Gram-positive erm(B) gene in a single porcine Campylobacter coli isolate (11), which carried no mutations in the 23S rRNA or in the rplD and rplV genes. erm(B) encodes a rRNA methylase and mediates highlevel macrolide resistance (MIC ϭ 512 g/ml) in Campylobacter, and it can be transferred between Campylobacter jejuni and Campylobacter coli via natural transformation (11). This was the first identification of a horizontally transferrable macrolide resistance mechanism in thermophilic Campylobacter spp.…”

mentioning

confidence: 91%

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Emergence of Multidrug-Resistant Campylobacter Species Isolates with a Horizontally Acquired rRNA Methylase

Wang

Zhang

Deng

et al. 2014

Antimicrob Agents Chemother

120

135

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Antibiotic-resistant Campylobacter constitutes a serious threat to public health, and resistance to macrolides is of particular concern, as this class of antibiotics is the drug of choice for clinical therapy of campylobacteriosis. Very recently, a horizontally transferrable macrolide resistance mediated by the rRNA methylase gene erm(B) was reported in a Campylobacter coli isolate, but little is known about the dissemination of erm(B) among Campylobacter isolates and the association of erm(B)-carrying isolates with clinical disease. To address this question and facilitate the control of antibiotic-resistant Campylobacter, we determined the distribution of erm(B) in 1,554 C. coli and Campylobacter jejuni isolates derived from food-producing animals and clinically confirmed human diarrheal cases. The results revealed that 58 of the examined isolates harbored erm(B) and exhibited high-level resistance to macrolides, and most were recent isolates, derived in 2011-2012. In addition, the erm(B)-positive isolates were all resistant to fluoroquinolones, another clinically important antibiotic used for treating campylobacteriosis. The erm(B) gene is found to be associated with chromosomal multidrug resistance genomic islands (MDRGIs) of Gram-positive origin or with plasmids of various sizes. All MDRGIs were transferrable to macrolide-susceptible C. jejuni by natural transformation under laboratory conditions. Molecular typing of the erm(B)-carrying isolates by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) identified diverse genotypes and outbreak-associated diarrheal isolates. Molecular typing also suggested zoonotic transmission of erm(B)-positive Campylobacter. These findings reveal an emerging and alarming trend of dissemination of erm(B) and MDRGIs in Campylobacter and underscore the need for heightened efforts to control their further spread.

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Section: Erm(b) In Campylobactersupporting

confidence: 90%

Section: Erm(b) In Campylobactermentioning

confidence: 78%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 91%

mentioning

confidence: 91%

See 3 more Smart Citations

Emergence of Multidrug-Resistant Campylobacter Species Isolates with a Horizontally Acquired rRNA Methylase

Wang

Zhang

Deng

et al. 2014

Antimicrob Agents Chemother

120

135

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Natural Competence and Horizontal Gene Transfer in Campylobacter

Golz

Stingl

2021

Current Topics in Microbiology and Immunology

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Thermophilic Campylobacter, in particular Campylobacter jejuni, C. coli and C. lari are the main relevant Campylobacter species for human infections. Due to their high capacity of genetic exchange by horizontal gene transfer (HGT), rapid adaptation to changing environmental and host conditions contribute to successful spreading and persistence of these foodborne pathogens. However, extensive HGT can exert dangerous side effects for the bacterium, such as the incorporation of gene fragments leading to disturbed gene functions. Here we discuss mechanisms of HGT, notably natural transformation, conjugation and bacteriophage transduction and limiting regulatory strategies of gene transfer. In particular, we summarize the current knowledge on how the DNA macromolecule is exchanged between single cells. Mechanisms to stimulate and to limit HGT obviously coevolved and maintained an optimal balance. Chromosomal rearrangements and incorporation of harmful mutations are risk factors for survival and can result in drastic loss of fitness. In Campylobacter, the restricted recognition and preferential uptake of free DNA from relatives are mediated by a short methylated DNA pattern and not by a classical DNA uptake sequence as found in other bacteria. A class two CRISPR-Cas system is present but also other DNases and restriction–modification systems appear to be important for Campylobacter genome integrity. Several lytic and integrated bacteriophages have been identified, which contribute to genome diversity. Furthermore, we focus on the impact of gene transfer on the spread of antibiotic resistance genes (resistome) and persistence factors. We discuss remaining open questions in the HGT field, supposed to be answered in the future by current technologies like whole-genome sequencing and single-cell approaches.

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Antimicrobial Resistance: An Overview

Shah,

Bhat,

Bhat

et al. 2024

Nanotechnology Based Strategies for Combating Antimicrobial Resistance

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Report of ribosomal RNA methylase gene erm(B) in multidrug-resistant Campylobacter coli

Abstract: To the best of our knowledge, this is the first report of a horizontally transferable macrolide resistance mechanism in thermophilic Campylobacter. Surveillance of erm(B) and its associated MDRGI in both C. coli and C. jejuni is urgently warranted.

Cited by 104 publications

References 35 publications

Emergence of Multidrug-Resistant Campylobacter Species Isolates with a Horizontally Acquired rRNA Methylase

Emergence of Multidrug-Resistant Campylobacter Species Isolates with a Horizontally Acquired rRNA Methylase

Natural Competence and Horizontal Gene Transfer in Campylobacter

Antimicrobial Resistance: An Overview

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