Reproducibility of saliva and plasma theophylline levels following single dose administration of two sustained release preparations.

Jackson, A. H.; Edwards, C. A.; Cope, A. S.; Stockley, R A; Purkiss, R.

doi:10.1111/j.1365-2125.1983.tb01522.x

Cited by 12 publications

(2 citation statements)

References 14 publications

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“…3). The coneentration of theophylline in saliva is reportedly neither influenced by salivary pH nor flow rates (4,5,18,20,21). Somewhat higher values for the protein binding of theophylline have been observed in previous studies, in which the pH of samples was apparently not controlled (16).…”

Section: Discussionmentioning

confidence: 83%

Determination of Theophylline in Saliva, Using Fluorescence Polarization Immunoassay (FPIA)

Niemann¹,

Oellerich²,

Schumann³

et al. 1985

Clinical Chemistry and Laboratory Medicine

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Theophylline concentrations were determined by fluorescence polarization immunoassay (FPIA) in saliva, serum and serum water of healthy volunteers and outpatients after administration of single , theophylline doses, and after the administration of several doses in order to establish a steady state. The ! FPIA allowed rapid and reliable theophylline determinations in saliva and serum water (between-days j coefficients of Variation: < 3%; recovery: 95 -103%). Salivary theophylline concentrations measured by l, ' FPIA in 30 samples agreed well with those determined by HPLC. Furthermore the results obtained by j ultrafiltration for the concentration of unbound theophylline in serum water were in good agreement with those determined by ultracentrifugation. The binding of theophylline by serum protein rose by about 25%, when the pH of the samples was increased from 7.0 to 8.0. After adjusting the pH to 7.4, average values for l theophylline binding to proteins at 25 °C ranged from 48.5 to 52.2% in serum samples from outpatients and healthy adults. Salivary theophylline concentrations correlated well with total and free serum theophylline concentrations in healthy adults and outpatients (r = 0.90-0.98). The theophylline concentration in saliva was on average about 20-30% higher than the unbound theophylline concentration in serum water. The l saliva/serum concentration ratio of theophylline showed some intersubject Variation (0.68 ± 0.08; ränge: 0.50-0.85). Using the inean saliva/serum concentration ratio of the patient group, steady state serum .theophylline concentrations were predicted from salivary levels with a mean error of 7.6% (ränge: 0.0-26.8%). The salivary theophylline concentration appears to be a suitable parameter for assessment of compliance, for identification of patients with inappropriate dosage, and for consequent dosage adjustment.

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Section: Discussionmentioning

confidence: 83%

Determination of Theophylline in Saliva, Using Fluorescence Polarization Immunoassay (FPIA)

Niemann¹,

Oellerich²,

Schumann³

et al. 1985

Clinical Chemistry and Laboratory Medicine

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“…Monitoring of serum theophylline levels is well established because of the narrow range of optimal serum concentration and the great interindividual differences in pharmacokinetics especially in ~h i l d r e n .~.~ Knowledge of theophylline levels is also useful in assessing and improving patient ~o m p l i a n c e .~ In order to make theophylline drug monitoring more convenient for the patient, numerous investigators have studied the accuracy of salivary theophylline levels in predicting serum levels and have obtained conflicting results . [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] In this study we have investigated the accuracy and predictive value of saliva theophylline measurements using fluorescence polarization immunoassay (FPIA), which has been proven to be a reliable The Seralyzer (reagent strip reflectance photometer method), which can be used for rapid theophylline determination in an ambulatory setting,21,22 was simultaneously tested. We tried to make sampling of saliva easier by using a special device.…”

Section: Introductionmentioning

confidence: 99%

Monitoring theophylline levels in serum and saliva using fluorescence polarization immunoassay and seralyzer

Schulte¹,

W²,

Schmidt³

et al. 1989

Pediatric Pulmonology

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The reliability and predictive value of salivary theophylline levels was investigated in 58 children who were treated with oral sustained-release theophylline preparations for bronchial asthma. Serum and saliva levels were assessed by fluorescence polarization immunoassay (FPIA), and Seralyzer (reagent strip reflectance photometer method). Forty-six paired serum and saliva samples from 28 patients (Group 1) were used to develop a linear regression formula. This formula was tested on 130 paired serum and saliva samples from 32 patients (Group 2). When saliva theophylline levels were assayed using FPIA, 95% of serum levels were predicted within 30% of the measured value. Using Seralyzer, 95% of serum levels were predicted within 45% of the measured value. For patients with three and more paired serum and saliva specimens, individual regression formulas were determined showing considerable variation. This study indicates that salivary theophylline levels provide for an estimation of serum levels if FPIA is used. The Seralyzer technique is only useful for serum theophylline measurements. Using a special device it is now easier to obtain saliva samples than by conventional methods.

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Serum/Saliva Correlations for Theophylline in Asthmatics

Blanchard¹,

Harvey²,

Morgan³

1991

The Journal of Clinical Pharma

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Theophylline levels in mixed saliva (both stimulated and unstimulated) were compared with total and free (unbound) serum theophylline levels in 28 asthmatic outpatients using theophylline regularly. Stimulated saliva predicted both total and unbound serum theophylline concentrations within +/- 1 microgram/mL in 62.5% and 92.9%, respectively, of the samples examined. In addition, the total serum levels could be used to predict unbound serum concentrations to within +/- 1 mg/L in 100% of the cases that were examined. These results indicate that saliva levels predict the unbound serum theophylline levels with greater accuracy and precision than they predict total serum theophylline levels. In addition, total serum levels can be used to reliably predict unbound serum levels. The use of mixed stimulated saliva is recommended as a reliable non-invasive method for monitoring unbound serum theophylline levels. The therapeutic range for saliva, which corresponds to the accepted total serum concentration range of 10-20 mg/L, is approximately 5.6-11.3 mg/L.

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Reproducibility of saliva and plasma theophylline levels following single dose administration of two sustained release preparations.

Cited by 12 publications

References 14 publications

Determination of Theophylline in Saliva, Using Fluorescence Polarization Immunoassay (FPIA)

Determination of Theophylline in Saliva, Using Fluorescence Polarization Immunoassay (FPIA)

Monitoring theophylline levels in serum and saliva using fluorescence polarization immunoassay and seralyzer

Serum/Saliva Correlations for Theophylline in Asthmatics

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