Reproducibility of the Histologic Diagnosis of Pneumonia Among a Panel of Four Pathologists

Corley, David E.; Kirtland, Steven H.; Winterbauer, Richard H.; Hammar, Samuel P.; Dail, David H.; Bauermeister, Donald E.; Bolen, John W.

doi:10.1378/chest.112.2.458

Cited by 96 publications

(40 citation statements)

References 12 publications

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“…Moreover, the recognition of histologic pneumonia varies among pathologists. In a study by Corley et al, the prevalence of pneumonia in postmortem open lung biopsies determined by each of four pathologists varied from 18% to 38% (40). Nonetheless, histopathology and lung tissue culture remain our best "gold standard" for the diagnosis of VAP.…”

Section: Microbiologic Diagnosismentioning

confidence: 99%

Ventilator-Associated Pneumonia: Diagnosis, Treatment, and Prevention

2006

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SUMMARY While critically ill patients experience a life-threatening illness, they commonly contract ventilator-associated pneumonia. This nosocomial infection increases morbidity and likely mortality as well as the cost of health care. This article reviews the literature with regard to diagnosis, treatment, and prevention. It provides conclusions that can be implemented in practice as well as an algorithm for the bedside clinician and also focuses on the controversies with regard to diagnostic tools and approaches, treatment plans, and prevention strategies.

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Section: Microbiologic Diagnosismentioning

confidence: 99%

Ventilator-Associated Pneumonia: Diagnosis, Treatment, and Prevention

2006

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“…The criteria used for histological definition of severe pneumonia included the presence of consolidation with pulmonary infiltrates and intense polymorphonuclear leukocyte and/or lymphocyte accumulations in bronchioles and adjacent alveoli seen in several adjacent lowpower microscope fields, with or without tissue necrosis (Corley et al, 1997).…”

Section: Methodsmentioning

confidence: 99%

Detection of Haemophilus influenzae type B DNA in a murine pneumonia model by in situ PCR

et al. 2008

Journal of Medical Microbiology

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This study estimated the value of in situ PCR (ISPCR) in the detection of Haemophilus influenzae type b (Hib) DNA in paraffin-embedded lung tissues of a murine pneumonia model. ICR mice were infected with Hib solution intranasally. In study group A (n520), physiological changes and the number of deaths were recorded for 7 consecutive days after infection. In study group B (n510), blood samples and lung tissues were obtained from the infected mice on the brink of death. In both groups, portions of the lung tissue were cultured for Hib, while other portions were submitted for histopathological studies. Conventional PCR, PCR followed by Southern blotting and ISPCR were performed to detect Hib in paraffin-embedded lung tissues. In control group A, six mice were inoculated intranasally with the same concentration of heat-inactivated Hib solution. In control group B, six healthy mice served as a blank control. Both control groups were managed using the same methods as those used in the study groups. The white blood cell count of the mice in the study group increased (F53.295, P,0.01), with a high neutrophil count (F50.127, P,0.05). In the histopathological study, various stages of pneumonia were found in the lung tissues of the infected mice examined by microscope; 80 % of the mice had moderate or severe pneumonia. Cultures of lung tissues in the study groups were all positive for Hib, while no bacteria were found in the control groups. Hib was detected in only 4 of 30 samples (13.3 %) of the study groups using conventional PCR, but in all 30 samples (100 %) using both Southern blotting and ISPCR. All three methods did not detect Hib in the control groups. Because of its sensitivity and specificity and its ability to locate the micro-organism, ISPCR can be considered suitable for the detection of Hib in paraffin-embedded lung tissues.

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“…There have been several studies of the diagnostic criteria for VAP in vivo (microbiology and cytopathology) and at autopsy (histopathology), and the results are unsettling (Corley et al 1997) (4177): www.intechopen.com …”

Section: Ventilator-associated Pneumonia (Vap)mentioning

confidence: 99%

The autopsy pathology of sepsis-related death

Lucas

2007

Current Diagnostic Pathology

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Reproducibility of the Histologic Diagnosis of Pneumonia Among a Panel of Four Pathologists

Cited by 96 publications

References 12 publications

Ventilator-Associated Pneumonia: Diagnosis, Treatment, and Prevention

Ventilator-Associated Pneumonia: Diagnosis, Treatment, and Prevention

Detection of Haemophilus influenzae type B DNA in a murine pneumonia model by in situ PCR

The autopsy pathology of sepsis-related death

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