Reproduction of Large Virulent Bacteriophages

Mathews, Christopher K.

doi:10.1007/978-1-4684-2712-7_3

Cited by 26 publications

(7 citation statements)

References 504 publications

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“…[E or QI d: lack of immunity to superinfection (42,197 (47) 18,000 13729,000 6846,000 9540,000 9610,000-o8,900 (196) 11,700-o10,000 (196) 21,200-o18,300 (196) 14733,000 14710,000 9629,000 (28) 10,500 (127) 53,700 (196) [psu+SB] The following general articles, not referred to in the table, are useful sources of additional information and references as follows: for the original assignment of essential gene functions (67); for early functions of phage-coded enzymes (128,129); for the original classification of gene functions in assembly of phage particles using in vitro complementation (63,219); for recent work on phage assembly (29,64,76,171,212,221); for gene functions in general (26, 34, 58, 136, 140, 155a, 178).…”

Section: Gene Classes and Gene Namesmentioning

confidence: 99%

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The genome of bacteriophage T4.

Wood¹,

Revel²

1976

Bacteriological Reviews

123

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Section: Gene Classes and Gene Namesmentioning

confidence: 99%

“…Ofthe metabolic gene functions, 22 are essential, as defined earlier. Six of these genes code for known proteins required for phage DNA synthesis (67), either as enzymes of nucleotide metabolism or components of the actual DNA replication and recombination machinery (4,128,129). A list of the known T4-induced enzymes is given in Table 3.…”

mentioning

confidence: 99%

The genome of bacteriophage T4.

Wood¹,

Revel²

1976

Bacteriological Reviews

123

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“…The data obtained with DNA polymerase I Klenow fragment and the susceptibility of individual RS1 EcoRI restriction fragments to BAL 31 nuclease suggest that these regions occur at a minimum within EcoRI restriction fragments A, C, D, and E. These four restriction fragments represent approximately 65% of the RS1 genome. Studies are currently in progress to determine whether these 3' hydroxyl groups are localized within specific regions of these restriction fragments or on one particular strand of the RS1 DNA, as is the case for the gaps found within one strand of E. coli wild-type bacteriophage T5 DNA (17,24). The data presented in this paper would strongly suggest that the single-stranded nicks or gaps within the RS1 DNA occur at a much higher frequency than those in either the wild type or mutants of T5 with increased frequencies of single-stranded chain interruptions (25).…”

Section: Resultsmentioning

confidence: 99%

Structure and physical map of Rhodopseudomonas sphaeroides bacteriophage RS1 DNA

Donohue¹,

Chory²,

Goldsand³

et al. 1985

J Virol

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We analyzed, by restriction endonuclease mapping and electron microscopy, the genome of the lytic Rhodopseudomonas sphaeroides-specific bacteriophage RS1 and characterized it as a linear molecule of approximately 60 to 65 kilobases. When the DNA from purified phage particles was examined by several independent methods, considerable size heterogeneity was apparent in the RS1 DNA. This size heterogeneity was concluded to be of biological origin, was independent of the specific host strain used to propagate virus, and was not due to the presence of host DNA within or nonspecifically associated with purified virions. In addition, treatment of RS1 DNA with either BAL 31 nuclease or DNA polymerase I Klenow fragment revealed that several distinct regions exist within the viral chromosome which contain free 3' hydroxyl groups. A restriction endonuclease map of the RS1 genome was constructed by using the restriction endonucleases EcoRI, ClaI, KpnI, BamHI, MluI, SmaI, and Bcll; thereby allowing the positioning of some 40 restriction sites within the viral genome. The results are discussed in terms of the significance and the possible biological origin of the unique features discovered within the phage RS1 DNA.

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“…All goF phages grow on the wild-type parental bacterium as well as on all T4+-permissive Caltech hospital strains (50), many of which are nonpermissive for T4 carrying deletions of phage genes that are nonessential in standard laboratory hosts (29).…”

Section: Resultsmentioning

confidence: 99%

Role of the host cell in bacteriophage T4 development. II. Characterization of host mutants that have pleiotropic effects on T4 growth

Stitt¹,

Revel²,

Lielausis³

et al. 1980

J Virol

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Mutant host-defective Escherichia coli that fail to propagate bacteriophage T4 and have a pleiotropic effect on T4 development have been isolated and characterized. In phage-infected mutant cells, specific early phage proteins are absent or reduced in amount, phage DNA synthesis is depressed by about 50%, specific structural phage proteins, including some tail and collar components, are deficient or missing, and host-cell lysis is delayed and slow. Almost all phage that can overcome the host block carry mutations that map in functionally undefined 'nonessential" regions of the T4 genome, most near gene 39. The mutant host strains are temperature sensitive for growth and show simultaneous reversion of the ts phenotype and the inability to propagate T4+. The host mutations are cotransduced with ilv (83 min) and may lie in the gene for transcription termination factor rho.

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Reproduction of Large Virulent Bacteriophages

Cited by 26 publications

References 504 publications

The genome of bacteriophage T4.

The genome of bacteriophage T4.

Structure and physical map of Rhodopseudomonas sphaeroides bacteriophage RS1 DNA

Role of the host cell in bacteriophage T4 development. II. Characterization of host mutants that have pleiotropic effects on T4 growth

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