Reproductive aspects and storage of semen in Camelidae

Bravo, P. Walter; Skidmore, J.A.; Zhao, Xingxu

doi:10.1016/s0378-4320(00)00158-5

Cited by 118 publications

(137 citation statements)

References 27 publications

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“…Mientras que comparado los porcentajes de gestación obtenidos en el presente estudio, con otros trabajos de inseminación artificial con semen congelado, procedente de colecciones de muestras por vagina artificial, en dosis de 0.25 o 0.5 mL con un cantidad variable de 3 a 7 millones de espermatozoides motiles a la descongelación, reportaron gestaciones en los primeros 30 y 60 días pos inseminación del 7 al 37% (Pérez 1996;Apaza et al 1999;Bravo et al 2000;Aller y col. 2003), estos resultados fueron similares a los encontrados en el presente estudio, esta similitud estaría dado porque los diferentes autores i n d i c a n h a b e r i n d u c i d o l a o v u l a c i ó n farmacológicamente aplicando hormonas de liberación fijando así el tiempo de ovulación para la inseminación artificial a tiempo fijo a las 30 h post aplicación. En forma similar en el presente estudio la inseminación artificial se realizo 30 h post inducción de ovulación con macho vasectomizado.…”

Section: Prueba De Fertilidad In-vivo De Los Espermatozoides Del Condunclassified

Viabilidad in vitro e in vivo de los espermatozoides congelados/descongelados del conducto deferente de Alpacas (vicugna pacos)

Durand¹,

Aragón²,

Guerra³

2016

Rev. Investig. Altoandin. - RIA -

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KEY WORDS:Viabilidad in vitro e in vivo de los espermatozoides congelados/descongelados del conducto deferente de alpacas (Vicugna pacos)Viability in vitro and invivo of sperm frozen/thawed from alpaca (Vicugna pacos) vas deferens El objetivo del presente estudio fue evaluar la viabilidad in-vitro e in-vivo de los espermatozoides procedente de los conductos deferentes de alpacas. Se utilizaron 2 reproductores como donadores de espermatozoides con desviación del conducto deferente. Los espermatozoides colectados se sometieron a la congelación y descongelación con el dilutor Triladyl®. Durante el procesamiento de los espermatozoides se evaluaron la motilidad y la prueba hipo-osmotica. En la inseminación se evaluó la proporción de gestaciones producidas. Los resultados fueron los siguientes: La motilidad de los espermatozoides para los reproductores 1 y 2 fueron: a los 37°C 65.16 y 63.37% sin diferencia (P>0.05), al enfriamiento (5°C) 52.63 y 48.31% similares (P>0.05) ya la descongelación 24.51 y 33.18% mostraron diferencia (P<0.05). A la prueba hipo-osmótica fueron; a los 37°C 51.55 y 52.98% similares (P>0.05), Al enfriamiento (5°C) 46.67 y 55.93% mostraron diferencia (P<0.05) y a la descongelación 20.06 y 32.18% con diferencia (P<0.05). Las gestaciones fueron: Con espermatozoides frescos diluidos 36.36% (4/11), con espermatozoides descongelados 25.00% (5/20) y con monta 54.54% (6/11), siendo similares (P>0.05). En conclusión los espermatozoides procedentes de los conductos deferentes soportan el congelamiento y a la inseminación artificial producen gestaciones.The aim of this study was to evaluate the in-vitro and in-vivo viability of sperm from the vas deferens alpacas. 2 males as sperm donors were used to offset the vas deferens. The collected sperm cells were subjected to freezing and thawing with dilutor Triladyl®. During processing of sperm motility and hypo-osmotic test they were evaluated. Insemination in the proportion of pregnancies produced was evaluated. The results were as follows: The sperm motility of males 1 and 2 were: at 37 ° C 65.16 and 63.37% with no difference (P> 0.05), cooling (5 ° C) 52.63 and 48.31% similar (P > 0.05) and thawing 24.51 and 33.18% showed no difference (P <0.05). The hypo-osmotic test were; at 37 ° C 51.55 and 52.98 similar percentage (P> 0.05), and cooling 55.93% 46.67 showed no difference (P <0.05) and thawing 20.06 and 32.18% with difference (P <0.05). Pregnancies were diluted with fresh sperm 36.36% (4/11), with defrosted sperm 25.00% (5/20) and 54.54% mounts (6/11), being similar (P> 0.05). In conclusion sperm from the vas deferens withstand freezing and insemination are able to produce pregnancies.

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Section: Prueba De Fertilidad In-vivo De Los Espermatozoides Del Condunclassified

Viabilidad in vitro e in vivo de los espermatozoides congelados/descongelados del conducto deferente de Alpacas (vicugna pacos)

Durand¹,

Aragón²,

Guerra³

2016

Rev. Investig. Altoandin. - RIA -

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“…Semen was collected from five dromedary camels between 0800 and 1000 h using a modified artificial vagina (30 cm long and 5 cm internal diameter, IMV, L'Aigle, France) as mentioned in previous studies [7][8][9]. Ejaculate contact with the rubber liner of the artificial vagina was avoided, since it has been reported that most rubber liners have a deleterious effect on camel spermatozoa [10].…”

Section: Semen Collection By Artificial Vaginamentioning

confidence: 99%

Survival and Fertility Rate of Cooled Dromedary Camel Spermatozoa Supplemented with Catalase Enzyme

Medan

Absy

Zeidan

et al. 2008

Journal of Reproduction and Development

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Abstract. The present study was planned to study the effects of addition of different concentrations of catalase enzyme (0, 250, 500 and 1,000 IU/ml) to cooled dromedary camel semen extended with tris-yolk-fructose extender on semen quality during storage at 5 C for up to 5 days. Conception rates of she-camels artificially inseminated with whole fresh or extended cooled dromedary camel semen with or without 500 IU/ml catalase enzyme were also estimated. The results showed that addition of catalase enzyme at concentrations of 250 or 500 IU/ml to extended cooled dromedary camel semen significantly increased (P<0.01) the percentage of sperm motility and significantly decreased (P<0.01) the percentages of dead spermatozoa, sperm abnormalities and acrosomal damage. The highest (P<0.01) percentage of sperm motility was recorded with extended cooled dromedary camel semen supplemented with catalase enzyme at a concentration of 500 IU/ml, and the lowest (P<0.01) value was recorded with catalase enzyme at a concentration of 1000 IU/ml. On the other hand, the lowest (P<0.01) percentages of dead spermatozoa, sperm abnormalities and acrosomal damage of spermatozoa were recorded with extended cooled dromedary camel semen supplemented with 500 IU/ml, and the highest (P<0.01) values were recorded with catalase enzyme at a concentration of 1,000 IU/ml. Advancement of the storage time at 5 C significantly decreased (P<0.01) the percentage of sperm motility and significantly increased (P<0.01) the percentages of dead spermatozoa, sperm abnormalities and acrosomal damage of spermatozoa. Moreover, the conception rates of she-camels artificially inseminated with whole fresh, extended cooled dromedary camel semen free-catalase enzyme and extended cooled dromedary camel semen supplemented with catalase enzyme at a concentration of 500 IU/ml were 46.15, 22.22 and 37.50%, respectively. In conclusion, the results show that addition of catalase enzyme at a concentration of 500 IU/ml to semen extender can be used as an agent for prolongation of dromedary camel sperm cell survival during storage at 5 C. Key words: Catalase enzyme, Dromedary camel, Semen quality, Spermatozoa (J. Reprod. Dev. 54: [84][85][86][87][88][89] 2008) rtificial insemination with preserved semen is a prerequisite for any breeding strategy to maximize spread of superior racing and draught potential of selected males of domestic species of camel. Artificial insemination is also useful to combat venereal diseases. It can be especially advantageous in camels because females exhibit follicular waves [1]. Pregnancy rates of 50-60% have been reported in camels inseminated with fresh diluted semen used within 30 min of collection [2] but conception rate decreases dramatically to 25-30% if the semen is not used within 24 hr.Great attention has been given to development of extenders that will preserve the functional activity of spermatozoa (viability and fertilizing ability) during storage at different temperatures. Various media have been recommended for dilution and preserv...

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“…O'Brien & Roth, 2000) or with the aid of intra-vaginal condom or vaginal sponge (e.g. Bravo et al, 2000), 2) artificial vagina (e.g. Gastal et al, 1996;Asher et al, 2000), 3) manual stimulation of either the rectum (e.g.…”

Section: The Malementioning

confidence: 99%

“…Oliveira et al, 2010). In camelids, possibly due to the absence of vesicular glands, sperm is also fairly viscous but it can be enzymatically liquefied (Bravo et al, 2000). Similar enzymatic liquefaction was also helpful when attempting to separate rhinoceros sperm from the seminal plasma, something that cannot be done efficiently with centrifugation alone in some of the ejaculates (Behr et al, 2009b).…”

Section: The Malementioning

confidence: 99%

Genome Banking for Vertebrates Wildlife Conservation

Saragusty¹

2012

Current Frontiers in Cryobiology

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Reproductive aspects and storage of semen in Camelidae

Cited by 118 publications

References 27 publications

Viabilidad in vitro e in vivo de los espermatozoides congelados/descongelados del conducto deferente de Alpacas (vicugna pacos)

Viabilidad in vitro e in vivo de los espermatozoides congelados/descongelados del conducto deferente de Alpacas (vicugna pacos)

Survival and Fertility Rate of Cooled Dromedary Camel Spermatozoa Supplemented with Catalase Enzyme

Genome Banking for Vertebrates Wildlife Conservation

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