Research Article Cloning and functional characterization of the HbSYR1 gene encoding a syntaxin-related protein in Tibetan hulless barley (Hordeum vulgare L. var. nudum HK. f.).

Xu, Qijun; Wang, Y.L.; Wei, Zexiu; Yuan, Hongjun; Zeng, Xingquan; Tashi, Nyima

doi:10.4238/gmr16038909

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…Unfortunately, to date, only limited progress has been made to enhance the stress tolerance in this crop. More importantly, several studies have focused on drought and cold tolerance enhancement in hulless barley (Deng et al 2013; Zeng et al 2016; Liang et al 2017; Xu et al 2017; Yuan et al 2017) but research on the salinity tolerance has been largely overlooked. The salinization of cropland soil in the Tibetan Plateau has become an increasingly serious concern for agricultural production (Wang et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Metabolite profiling in two contrasting Tibetan hulless barley cultivars revealed the core salt-responsive metabolome and key salt-tolerance biomarkers

Wang

Zeng

et al. 2019

AoB PLANTS

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Salinity stress represents one of the most harmful abiotic stresses for agricultural productivity. Tibetan hulless barley is an important economic crop widely grown in highly stressful conditions in the Qinghai-Tibet Plateau and is often challenged by salinity stress. To investigate the temporal metabolic responses to salinity stress in hulless barley, we performed a widely targeted metabolomic analysis of 72 leaf samples from two contrasting cultivars. We identified 642 compounds 57 % of which were affected by salt stress in the two cultivars, principally amino acids and derivatives, organic acids, nucleotides, and derivatives and flavonoids. A total of 13 stress-related metabolites including piperidine, L-tryptophan, L-glutamic acid, L-saccharopine, L-phenylalanine, 6-methylcoumarin, cinnamic acid, inosine 5′-monophosphate, aminomalonic acid, 6-aminocaproic acid, putrescine, tyramine and abscisic acid (ABA) represent the core metabolome responsive to salinity stress in hulless barley regardless of the tolerance level. In particular, we found that the ABA signalling pathway is essential to salt stress response in hulless barley. The high tolerance of the cultivar 0119 is due to a metabolic reprogramming at key stress times. During the early salt stress stages (0–24 h), 0119 tended to save energy through reduced glycolysis, nucleotide metabolism and amino acid synthesis, while increased antioxidant compounds such as flavonoids. Under prolonged stress (48–72 h), 0119 significantly enhanced energy production and amino acid synthesis. In addition, some important compatible solutes were strongly accumulated. By comparing the two cultivars, nine salt-tolerance biomarkers, mostly unreported salt-tolerance compounds in plants, were uncovered. Our study indicated that the salt tolerant hulless barley cultivar invokes a tolerance strategy which is conserved in other plant species. Overall, we provide for the first time some extensive metabolic data and some important salt-tolerance biomarkers which may assist in efforts to improve hulless barley tolerance to salinity stress.

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Section: Introductionmentioning

confidence: 99%

Metabolite profiling in two contrasting Tibetan hulless barley cultivars revealed the core salt-responsive metabolome and key salt-tolerance biomarkers

Wang

Zeng

et al. 2019

AoB PLANTS

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“…nudum Hook. f.), also called naked barley, is an important cereal crop in Tibet Plateau (Xu et al 2016). It has been served as a healthy food for human consumption and animal feed for over thousands of years.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide DNA Methylation Dynamics During Drought Responsiveness in Tibetan Hulless Barley

Dunzhu

et al. 2023

J Plant Growth Regul

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Differences in drought stress tolerance within diverse grass genotypes have been attributed to epigenetic modifications. DNA methylation is an important epigenetic alteration regulating responses to drought stress. However, its effects on drought tolerance are poorly understood in Tibetan hulless barley. Here, bisulfite sequencing was conducted to profile the DNA methylation patterns of drought-tolerant variety (XL) and drought-sensitive (DQ) under drought and control conditions. A total of 5843 million reads were generated. We found the significant genome-wide changes in CHH methylation rates between XL and DQ, while CG or CHG methylation rates did not. Besides that, the two contrasting varieties do reveal distinct responses to drought stress in antioxidant activities and differentially methylated regions (DMRs). Genes in drought-tolerant varieties XL are rapidly and significantly methylated when exposed to drought stimulus. These DMRs-related genes in XL are significantly enriched in defense response and response to stimuli via gene-ontology enrichment analysis. Then, we focused on 1003 transcription factors and identified 15 specific DMRs-related transcription factors exhibiting specific methylation changes under drought stimuli. Finally, we identified three DMRs-related TFs (HvRR12, HvRR2, and HvCSP41B), where Arabidopsis homologs involve in responses to drought conditions. Altogether, abiotic stresses could be rapidly respond and mediated by methylation of transcription factors in hulless barely.

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“…In hulless barley, integrative functional genomics approach was employed to elucidate the molecular basis of drought tolerance. The genes HbSINA4 and HbSYR1 were cloned and functionally validated as important genes for drought tolerance and water retention [9, 10]. Based on transcriptome analysis, 853 differentially expressed genes were detected as involved in drought response and categorized into nine clusters enriched in various biological pathways in a drought tolerant hulless barley genotype [11].…”

Section: Introductionmentioning

confidence: 99%

Time-Course Comparative Metabolite Profiling under Osmotic Stress in Tolerant and Sensitive Tibetan Hulless Barley

Yuan

Zeng

Shi³

et al. 2018

BioMed Research International

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Tibetan hulless barley is widely grown in the extreme environmental conditions of the Qinghai-Tibet Plateau which is characterized by cold, high salinity, and drought. Osmotic stress always occurs simultaneously with drought and its tolerance is a vital part of drought tolerance. The diversity of metabolites leading to osmotic stress tolerance was characterized using widely-targeted metabolomics in tolerant (XL) and sensitive (D) accessions submitted to polyethylene glycol. XL regulated a more diverse set of metabolites than D, which may promote the establishment of a robust system to cope with the stress in XL. Compounds belonging to the group of flavonoids, amino acids, and glycerophospholipids constitute the core metabolome responsive to the stress, despite the tolerance levels. Moreover, 8 h appeared to be a critical time point for stress endurance involving a high accumulation of key metabolites from the class of nucleotide and its derivative which provide the ultimate energy source for the synthesis of functional carbohydrates, lipids, peptides, and secondary metabolites in XL. This intrinsic metabolic adjustment helped XL to efficiently alleviate the stress at the later stages. A total of 22 diverse compounds were constantly and exclusively regulated in XL, representing novel stress tolerance biomarkers which may help improving stress tolerance, especially drought, in hulless barley.

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Research Article Cloning and functional characterization of the HbSYR1 gene encoding a syntaxin-related protein in Tibetan hulless barley (Hordeum vulgare L. var. nudum HK. f.).

Cited by 4 publications

References 12 publications

Metabolite profiling in two contrasting Tibetan hulless barley cultivars revealed the core salt-responsive metabolome and key salt-tolerance biomarkers

Metabolite profiling in two contrasting Tibetan hulless barley cultivars revealed the core salt-responsive metabolome and key salt-tolerance biomarkers

Genome-Wide DNA Methylation Dynamics During Drought Responsiveness in Tibetan Hulless Barley

Time-Course Comparative Metabolite Profiling under Osmotic Stress in Tolerant and Sensitive Tibetan Hulless Barley

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