Research Article The effect of acetate Ringer's solution on inflammatory factors and NF-ÎºB signal pathway in the liver of hemorrhagic shock rats

Wang, Z. J.

doi:10.4238/gmr16039906

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…Cannabidiol alleviated the hemorrhagic shock-induced neural apoptosis by the activation of PI3K/AKT signaling pathway (34). In THS rat model, the inactivation of NF-κB p65 protein and TLR4/NF-κB signaling pathway could reverse the expression imbalance of inflammatory mediators and reduce organ damage caused by THS (35). Here, FIG.…”

Section: Discussionmentioning

confidence: 84%

Effect of Mir-21-3p on Intestinal Injury in Rats With Traumatic Hemorrhagic Shock Resuscitated With the Sodium Bicarbonate Ringer's Solution

Li,

Jiang,

Qiu

et al. 2024

Shock

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Background This study aims to determine the impact and mechanism of miR-21-3p on intestinal injury and intestinal glycocalyx during fluid resuscitation in traumatic hemorrhagic shock (THS), and the different impacts of sodium lactate Ringer's solution (LRS) and sodium bicarbonate Ringer's solution (BRS) for resuscitation on intestinal damage. Methods A rat model of THS was induced by hemorrhage from the left femur fracture. The pathological changes of intestinal tissues and glycocalyx structure were observed by hematoxylin-eosin (H&E) staining and transmission electron microscope. MiR-21-3p expression in intestinal tissues was detected by real-time quantitative polymerasechain reaction (RT-qPCR). The expression of glycocalyx-, cell junction- and PI3K/Akt/NF-κB signaling pathway- related proteins was analyzed by western blot. Results MiR-21-3p expression was increased in THS rats, which was suppressed by resuscitation with BRS. BRS or LRS aggravated the intestinal injury and damaged intestinal glycocalyx in THS rats. The expression of SDC-1, HPA, β-catenin, MMP2 and MMP9 was upregulated, the expression of E-cad was downregulated and the PI3K/Akt/NF-κB signaling pathway was activated in THS rats, which were further aggravated by BRS or LRS. The side effect of LRS was more serious than BRS. MiR-21-3p overexpression deteriorated the injury of intestinal tissues and intestinal glycocalyx, increased the expression of SDC-1, HPA, β-catenin, MMP2 and MMP9 while decreased E-cad expression, and activated the PI3K/Akt/NF-κB signaling pathway in BRS-resuscitated THS rats. Conclusion MiR-21-3p aggravated intestinal tissue injury and intestinal glycocalyx damage through activating PI3K/Akt/NF-κB signaling pathway in rats with THS resuscitated with BRS.

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Section: Discussionmentioning

confidence: 84%

Effect of Mir-21-3p on Intestinal Injury in Rats With Traumatic Hemorrhagic Shock Resuscitated With the Sodium Bicarbonate Ringer's Solution

Li,

Jiang,

Qiu

et al. 2024

Shock

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“…The inhibition of IκB kinase/NF-κB signaling by BAY11-7082 was shown to improve the proinflammatory response of microvascular endothelial cells in mice with hemorrhagic shock ( 56 ). Previous studies by our team have shown that the inhibition of NF-κB P65 protein and activation of toll like receptor 4 (TLR4)/NF-κB signaling pathway reduced pro-inflammatory cytokines (TNF-α, IL-1, and IL-6) and increased anti-inflammatory cytokines (IL-4 and IL-10) in hemorrhagic shock rats and thus reversed the imbalance of inflammatory mediators and reduced organ damage caused by THS ( 57 , 58 ).…”

Section: Discussionmentioning

confidence: 97%

Effect of miR-21-3p on lung injury in rats with traumatic hemorrhagic shock resuscitated with sodium bicarbonate Ringer’s solution

Xu¹,

Qiu²,

Zheng³

et al. 2022

Ann Transl Med

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Background: Restricted fluid resuscitation is the most important early method for treating traumatic hemorrhagic shock (THS). This study sought to explore whether micro ribonucleic acid (miR)-21-3p affected resuscitated THS rats by regulating the glycocalyx and inflammation. Methods: MiRNAs extracted from the lung tissues were analyzed by miRNA microarray assays. A rat model of THS was induced by hemorrhage from a left femur fracture. The pathological change in the lung tissues and glycocalyx structure was observed by hematoxylin and eosin staining and a transmission electron microscope examination. The miR-21-3p expression in the lung tissues and serum was detected by real-time quantitative polymerase chain reaction (RT-qPCR). The levels of glycocalyx-related factors and inflammation-related factors were determined by enzyme linked immunosorbent assays. The expression of glycocalyx-related proteins, cell junction-related proteins, and the phosphatidylinositol 3-kinase (PI3K)/ protein kinase B (Akt)/nuclear factor kappa B (NF-κB) signaling pathway-related proteins was analyzed by Western blot.Results: After RT-qPCR verification, the variation trend of miR-21-3p was in line with expected trends.The mean arterial pressure (MAP) and heart rate (HR) were decreased, and the lung injury and damage to the glycocalyx were all aggravated in the THS rats resuscitated with sodium bicarbonate Ringer's solution (BRS) or sodium lactate Ringer's solution (LRS). The expression of miR-21-3p was decreased in the THS rats resuscitated with BRS and increased in the THS rats resuscitated with LRS, and the upregulation of miR-21-3p further decreased the MAP and HR, and increased the levels of syndecan-1 (SDC-1), heparanase-1 (HPA1), interleukin (IL)-6, IL-1β, and tumor necrosis factor alpha (TNF-α) in the serum of the THS rats resuscitated with BRS. The upregulation of miR-21-3p also increased the expression of SDC-1, HPA1, β-catenin, matrix metallopeptidase (MMP)2, and MMP9, but decreased the expression of E-cadherin (E-cad) and activated the PI3K/Akt/NF-κB signaling pathway in the THS rats resuscitated with BRS and transfected with miR-21-3p compared to that of the THS rats resuscitated with BRS and transfected with miR-negative control (NC).Conclusions: miR-21-3p promoted inflammation and glycocalyx damage by activating the PI3K/Akt/NF-κB signaling pathway, thereby aggravating the lung injury in the THS rats resuscitated with BRS.

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Research Article The effect of acetate Ringer's solution on inflammatory factors and NF-ÎºB signal pathway in the liver of hemorrhagic shock rats

Cited by 2 publications

References 21 publications

Effect of Mir-21-3p on Intestinal Injury in Rats With Traumatic Hemorrhagic Shock Resuscitated With the Sodium Bicarbonate Ringer's Solution

Effect of Mir-21-3p on Intestinal Injury in Rats With Traumatic Hemorrhagic Shock Resuscitated With the Sodium Bicarbonate Ringer's Solution

Effect of miR-21-3p on lung injury in rats with traumatic hemorrhagic shock resuscitated with sodium bicarbonate Ringer’s solution

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