Research Note: The immune enhancement ability of inulin on ptfA gene DNA vaccine of avian Pasteurella multocida

Gong, Qianhong; Peng, Yonggang; Niu, Mingfu; Qin, Chen

doi:10.1016/j.psj.2020.03.006

Cited by 6 publications

(3 citation statements)

References 8 publications

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“…A similar phenomenon was also found in Salmonella studies, suggesting that the protective role of antibodies specific to the LPS is not indispensable for live attenuated S. Typhimurium vaccines [ 29 , 37 ]. Some membrane proteins of P. multocida , including PlpE, Cp39, OmpH, PtfA and FHAB2, have been proven to be protective antigens that stimulate protective immunity against FC [ 38 – 42 ]. It would be of interest to examine the role and contribution of these antigens in the induction of protective immunity by live attenuated P. multocida vaccines.…”

Section: Discussionmentioning

confidence: 99%

Immunogenicity and protection of a Pasteurella multocida strain with a truncated lipopolysaccharide outer core in ducks

Zhao

Yang

Shen

et al. 2022

Vet Res

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Pasteurella multocida infection frequently causes fowl cholera outbreaks, leading to huge economic losses to the poultry industry worldwide. This study developed a novel live attenuated P. multocida vaccine strain for ducks named PMZ2 with deletion of the gatA gene and first four bases of the hptE gene, both of which are required for the synthesis of the lipopolysaccharide (LPS) outer core. PMZ2 produced a truncated LPS phenotype and was highly attenuated in ducks with a > 105-fold higher LD50 than the wild-type strain. PMZ2 colonized the blood and organs, including the spleen, liver and lung, at remarkably reduced levels, and its high dose of oral infection did not cause adverse effects on body temperatures and body weights in ducks. To evaluate the vaccine efficacy of the mutant, ducklings were inoculated orally or intranasally with PMZ2 or PBS twice and subsequently subjected to a lethal challenge. Compared with the PBS control, PMZ2 immunization stimulated significantly elevated serum IgG, bile IgA and tracheal IgA responses, especially after the boost immunization in both the oral and intranasal groups, and the induced serum had significant bactericidal effects against the wild-type strain. Furthermore, the two PMZ2 immunization groups exhibited alleviated tissue lesions and significantly decreased bacterial loads in the blood and organs compared with the PBS group post-challenge. All the ducks in the PMZ2 oral and intranasal groups survived the challenge, while 70% of ducks in the PBS group succumbed to the challenge. Thus, the P. multocida mutant with mutation of the gatA gene and part of the hptE gene proved to be an effective live attenuated vaccine candidate for prevention of fowl cholera in ducks.

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Section: Discussionmentioning

confidence: 99%

Immunogenicity and protection of a Pasteurella multocida strain with a truncated lipopolysaccharide outer core in ducks

Zhao

Yang

Shen

et al. 2022

Vet Res

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“…The remaining in vivo studies in this field of research explored, to a lesser extent, the novel use of bacterial DNA [85] and natural compounds [86,87]. Different compounds of plant and animal origin converged with varying degrees of experimental development in this last small group of substances.…”

Section: In Vivo Evaluation Of New Vaccines Against P Multocidamentioning

confidence: 99%

Global commercialization and research of veterinary vaccines against Pasteurella multocida: 2015–2022 technological surveillance

Odio

Delgado

2023

Vet World

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Background and Aim: Pasteurella multocida can infect a multitude of wild and domesticated animals, bacterial vaccines have become a crucial tool in combating antimicrobial resistance (AMR) in animal production. The study aimed to evaluate the current status and scientific trends related to veterinary vaccines against Pasteurella multocida during the 2015–2022 period. Materials and Methods: The characteristics of globally marketed vaccines were investigated based on the official websites of 22 pharmaceutical companies. VOSviewer® 1.6.18 was used to visualize networks of coauthorship and cooccurrence of keywords from papers published in English and available in Scopus. Results: Current commercial vaccines are mostly inactivated (81.7%), adjuvanted in aluminum hydroxide (57.8%), and designed to immunize cattle (33.0%). Investigational vaccines prioritize the inclusion of attenuated strains, peptide fragments, recombinant proteins, DNA as antigens, aluminum compounds as adjuvants and poultry as the target species. Conclusion: Despite advances in genetic engineering and biotechnology, there will be no changes in the commercial dominance of inactivated and aluminum hydroxide-adjuvanted vaccines in the short term (3–5 years). The future prospects for bacterial vaccines in animal production are promising, with advancements in vaccine formulation and genetic engineering, they have the potential to improve the sustainability of the industry. It is necessary to continue with the studies to improve the efficacy of the vaccines and their availability. Keywords: adjuvant, Pasteurella multocida, strain, technological surveillance, vaccine.

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“…DNA was added in an amount of 5.0 μL (100-150 ng). Studies were performed on a thermal cycler 2720 (Applied Biosystems, USA) with the temperature Molecular genetic studies were performed to identify the presence of the genes toxA, which is responsible for producing a dermonecrotic toxin, and ptfA, which encodes production of the products that assemble to form type 4 fimbriae on the bacterial surface and are responsible for biofilm formation (Devi et al, 2018;Gong et al, 2020).…”

Section: Thismentioning

confidence: 99%

Studija znakova patogenosti kod Pasteurella multocida, izolirane iz životinja različitih vrsta

et al. 2022

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A significant number of microorganisms in natural and artificial environments exist in a structured formation – biofilm. This formation attaches to a certain surface, particularly the epithelium. The ability to form a similar structure has been observed in Pasteurella multocida, the causative agent of anthropozoonoses that affect domestic and wild animals, birds, companion animals and humans. The spectrum of pathogenetic action of P. multocida is wide and associated with the development of respiratory and multisystemic pathology, bacteraemia and other manifestations. Timely detection of P. multocida and treatment of the diseases it causes in farm and domestic animals is important to limit economic losses and improve social security. The main objective of this study was to determine the pathogenicity of P. multocida, its ability to form a biofilm, its resistance to antibiotics, and to identify the genes responsible for the formation of dermonecrotic toxin and biofilm formation. The paper presents the results of a study of 11 isolates of P. multocida: six isolates (54.5%) from rabbits, two isolates (18.2%) from dogs, two isolates (18.2%) from cats, and one isolate from pigs (9.2%). In all isolates, the gene ptfA was detected. This gene encodes the formation of type 4 fimbriae and participates in the formation of the biofilm, and the studied cultures in vitro formed a biofilm of different densities. The genome of eight isolates (72.7%) included the toxA gene (provides the formation of dermonecrotic toxin), while 45.4% of isolates had a complete set of the studied signs of pathogenicity, both in phenotypic (biofilm formation, mortality for laboratory animals) and genotypic (presence of toxA, ptfA) traits, and three isolates (27.3%) showed signs of multidrug resistance. The virulence of the toxA-negative isolates of P. multocida was lower than in toxA-positive isolates. The culture with the highest virulence (0.5x 101 CFU) and extreme resistance to antibiotics formed a biofilm of the highest density. The association of the gene in the biofilm-producing mechanism needs further evaluation, and further research is needed to identify the relationships between pathogens in Pasteurella multocida isolates from different species of animals and humans.

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Research Note: The immune enhancement ability of inulin on ptfA gene DNA vaccine of avian Pasteurella multocida

Cited by 6 publications

References 8 publications

Immunogenicity and protection of a Pasteurella multocida strain with a truncated lipopolysaccharide outer core in ducks

Immunogenicity and protection of a Pasteurella multocida strain with a truncated lipopolysaccharide outer core in ducks

Global commercialization and research of veterinary vaccines against Pasteurella multocida: 2015–2022 technological surveillance

Studija znakova patogenosti kod Pasteurella multocida, izolirane iz životinja različitih vrsta

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