Research on antioxidant effects and estrogenic effect of formononetin from Trifolium pratense (red clover)

Mu, Hui; Bai, Yue; Wang, S.-T.; Zhu, Zhanfang; Zhang, Y.-W.

doi:10.1016/j.phymed.2008.07.005

Cited by 123 publications

(83 citation statements)

References 26 publications

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“…Besides, being a natural compound, formononetin may have little toxic effect. What's more, formononetin possesses antioxidant property [44] , and it increases the expression of eNOS [9] and lowers blood lipid [45] , which will be beneficial in preventing and treating the complications of hypertension, such as cardiac and vascular remodeling, lipid abnormalities and atherosclerosis. Therefore, formononetin may be of value in treating and preventing hypertension.…”

Section: Discussionmentioning

confidence: 99%

Vasorelaxant and antihypertensive effects of formononetin through endothelium-dependent and -independent mechanisms

Sun

Cao

2011

Acta Pharmacol Sin

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Aim: To investigate the mechanisms underlying the vasorelaxant effect of formononetin, an O-methylated isoflavone, in isolated arteries, and its antihypertensive activity in vivo. Methods: Arterial rings of superior mesenteric arteries, renal arteries, cerebral basilar arteries, coronary arteries and abdominal aortas were prepared from SD rats. Isometric tension of the arterial rings was recorded using a myograph system. Arterial pressure was measured using tail-cuff method in spontaneously hypertensive rats. Results: Formononetin (1-300 μmol/L) elicited relaxation in arteries of the five regions that were pre-contracted by KCl (60 mmol/L), U46619 (1 μmol/L) or phenylephrine (10 μmol/L). The formononetin-induced relaxation was reduced by removal of endothelium or by pretreatment with L-NAME (100 μmol/L). Under conditions of endothelium denudation, formononetin (10, 30, and 100 μmol/L) inhibited the contraction induced by KCl and that induced by CaCl 2 in Ca 2+ -free depolarized medium. In the absence of extracellular Ca 2+ , formononetin (10, 30, and 100 μmol/L) depressed the constriction caused by phenylephrine (10 μmol/L), but did not inhibit the tonic contraction in response to the addition of CaCl 2 (2 mmol/L). The contraction caused by caffeine (30 mmol/L) was not inhibited by formononetin (100 μmol/L). Formononetin (10 and 100 μmol/L) reduced the change rate of Ca 2+ -fluorescence intensity in response to KCl (50 mmol/L). In spontaneously hypertensive rats, formononetin (5, 10, and 20 mg/kg) slowly lowered the systolic, diastolic and mean arterial pressure. Conclusion: Formononetin causes vasodilatation via two pathways: (1) endothelium-independent pathway, probably due to inhibition of voltage-dependent Ca 2+ channels and intracellular Ca 2+ release; and (2) endothelium-dependent pathway by releasing NO. Both the pathways may contribute to its antihypertensive effect.

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Section: Discussionmentioning

confidence: 99%

Vasorelaxant and antihypertensive effects of formononetin through endothelium-dependent and -independent mechanisms

Sun

Cao

2011

Acta Pharmacol Sin

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“…This is the key process to guarantee the purity and yield of formononetin [12]. Studies indicate that formononetin from Trifolium pratense L. (red clover) processes excellent activity to scavenge free radicals and that its administration can enhance the tissue enzymatic and nonenzymatic defenses of mice and thus prevent the lipid peroxidation formation [13].…”

Section: Introductionmentioning

confidence: 99%

Extraction and purification of formonometin from <i>Trifolium pratense</i> L: Physicochemical properties of its complex with lecithin

Brad¹,

Zhang²,

Gao³

2017

Trop. J. Pharm Res

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“…Many studies evaluated antioxidant activity of phenolic compounds from different fruit and vegetable species, herbs, tea, wine, and medicinal plants (Sato et al 1996;Wang et al 1996;Pietta et al 1998). Beside this in the literature there are several records describing the antioxidant activity of the foreign crops (Sivakumaran et al 2004;Mu et al 2009;Sabudak & Guler 2009). …”

Section: Introductionmentioning

confidence: 99%

Proteinase inhibition and antioxidant activity of selected forage crops

et al. 2010

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The hypothesis of the possible therapeutic potential of selected species of forage crops is discussed. Extracts from genotypes of Anthyllis sp., Astragalus sp., Coronilla sp., Lotus sp., Medicago sp., Melilotus sp., Onobrychis sp. and Trifolium sp. were prepared and tested for proteinase inhibition and antioxidant activities. We found that Trifolium pratense accession POLKIE99-3 expressed the highest relative trypsin inhibition activity (80.0%) compared to standards. The highest thrombin inhibition activity (81.4%) was detected in the Medicago sativa old cultivar Hodonínka, whereas the highest relative urokinase inhibition activity (62.5%) was expressed by the local population Nitranka. Relatively high antioxidant properties of Trifolium sp. accessions, Trifolium pratense genetic resources SVKZAH98-40, were of interest. Results of this study confirmed that there are significant differences in proteinase inhibition and antioxidant activity among important selected agricultural crops. The present paper may also be the starting point of the research aimed for development of new functional food and nutraceuticals, and/or of the research focused on new secondary metabolites with potential as bioactive compounds.Key words: trypsin; thrombin; urokinase; inhibition; antioxidant; activity; forage crops.Abbreviations: AOX, antioxidant activity; BBI, Bowman-Birk inhibitor; DMSO, dimethyl sulphoxide; Rel%BT, relative percentage value of activity to standardized black tea extract; Rel%Q, relative percentage value of activity to standard quercetin; SHE, sunflower oil hydrolysate ethanol; SITh, selectivity index of thrombin; SIU, selectivity index of urokinase; ThIA, thrombin inhibition activity; TrIA, trypsin inhibition activities; UIA, urokinase inhibition activity.

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Research on antioxidant effects and estrogenic effect of formononetin from Trifolium pratense (red clover)

Cited by 123 publications

References 26 publications

Vasorelaxant and antihypertensive effects of formononetin through endothelium-dependent and -independent mechanisms

Vasorelaxant and antihypertensive effects of formononetin through endothelium-dependent and -independent mechanisms

Extraction and purification of formonometin from <i>Trifolium pratense</i> L: Physicochemical properties of its complex with lecithin

Proteinase inhibition and antioxidant activity of selected forage crops

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