Resistance mechanism of <i>Fusarium fujikuroi</i> to phenamacril in the field

Hou, Yiping; Qu, Xiang-Pu; Mao, Xuewei; Kuang, Jing; Duan, Yabing; Song, Xiu-Shi; Wang, Jianxin; Chen, Changjun; Zhou, Mingguo

doi:10.1002/ps.4742

Cited by 67 publications

(75 citation statements)

References 24 publications

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“…Phenamacril is a novel cyanoacrylate fungicide. It has an excellent antifungal effect on Fusarium species by inhibiting the ATPase activity of their myosin class I motor domains and has a good control effect on FHB . However, previous studies have shown that F. graminearum has a medium risk of resistance to this fungicide, which phenamacril cannot be used alone .…”

Section: Introductionmentioning

confidence: 99%

Resistance risk assessment for Fusarium graminearum to pydiflumetofen, a new succinate dehydrogenase inhibitor

Sun

Cui

Tian

et al. 2019

Pest Management Science

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Background Pydiflumetofen is a new generation succinate dehydrogenase inhibitor currently undergoing the process of registration in China for the control of Fusarium head blight in wheat. A resistance risk assessment of Fusarium graminearum to pydiflumetofen was undertaken in this study. Results A total of 75 pydiflumetofen‐resistant mutants were generated through spontaneous selection and displayed high resistance with an average resistance factor (RF) value of 78. Four mutants were generated through UV mutagenesis and displayed very high resistance with an RF value >1000. The sequence analysis results for Sdh genes and fitness studies revealed the existence of four types of mutations. In particular, 32 spontaneous selection mutants (SP mutants) had an arginine (R) to histidine (H) transition at position 86 in FGSdhC, resulting in seriously reduced fitness. Seven SP mutants had an R to cysteine (C) transition at position 86 in FGSdhC, resulting in reduced fitness. Thirty‐six SP mutants had an alanine (A) to valine (V) transition at position 83 in FGSdhC and had no fitness penalties. The efficacy of pydiflumetofen towards a mutant carrying A83V in FGSdhC in vivo was significantly decreased at 42.7%. Four UV mutants had no mutations on all Sdh genes and no fitness penalties. Cross‐resistance among boscalid, fluopyram and pydiflumetofen was observed. Conclusion Sdhc mutations were found and other target site resistance may be present in laboratory PR mutants of F. graminearum. An overall moderate risk of resistance development in F. graminearum was recommended for pydiflumetofen. © 2019 Society of Chemical Industry

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Section: Introductionmentioning

confidence: 99%

Resistance risk assessment for Fusarium graminearum to pydiflumetofen, a new succinate dehydrogenase inhibitor

Sun

Cui

Tian

et al. 2019

Pest Management Science

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“…Benzimidazole fungicides bind to β‐tubulin to inhibit mycelial growth of plant pathogenic fungi, and the resistance mechanism is the point mutation of amino acids encoding the β‐tubulin in Botrytis cinerea and Sclerotinia sclerotiorum (Liu et al, , ; Zhu, Zhou, Li, Zhu, & Ma, ), or amino acids encoding the β 2 ‐tubulin gene in F. graminearum (Liu, Duan, Ge, Chen, & Zhou, ; Liu et al, ). The active target of the cyanoacrylate fungicide phenamacril is myosin 5 of the FGSC, and the point mutation in its coding gene leads to resistance of the FGSC to phenamacril (Hou et al, ; Li et al, ; Zheng et al, ). Therefore, there was no cross‐resistance between metconazole and carbendazim or phenamacril in F. graminearum , and positive cross‐resistance relationship between metconazole and epoxiconazole, difenoconazole or tebuconazole in F. graminearum .…”

Section: Discussionmentioning

confidence: 99%

“…Owing to the prolonged use of only benzimidazole fungicides, carbendazim‐resistant isolates have developed in many plant pathogenic fungi, which leads to a decline in the efficacy of fungicides and the difficulty in controlling FHB (Liu, Che, & Chen, ; Liu et al, ; Qiu et al, , ; Yuan & Zhou, ). Although the cyanoacrylate fungicide phenamacril has high activity against the FGSC, previous studies have confirmed that it has a very high level of resistance risk for the FGSC and resistant isolates have been reported in F. fujikuroi , another member of the FGSC, in fields in China (Duan et al, ; Hou et al, ; Zhang et al, ).…”

Section: Introductionmentioning

confidence: 99%

Baseline sensitivity of Fusarium graminearum from wheat fields in Henan, China, to metconazole and analysis of cross resistance with carbendazim and phenamacril

Liu

et al. 2019

Journal of Phytopathology

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The filamentous plant pathogenic fungus Fusarium graminearum is one of the most important pathogens causing Fusarium head blight (FHB) in wheat in the Henan Province of China. Metconazole is among the demethylation inhibitor (DMI) fungicides with a higher inhibitory activity on the mycelial growth of F. graminearum. In 2016 and 2017, 119 single spore isolates of F. graminearum, prior to being exposed to metconazole, were recovered from 52 wheat fields near 11 cities in Henan Province. The inhibitory activity of metconazole on the mycelia of the Henan F. graminearum population was determined, and EC50 values were calculated. The range of EC50 values of the Henan F. graminearum population to metconazole was 0.0103 to 0.0775 μg/ml with an average EC50 value of 0.0293 ± 0.0114 μg/ml. The sensitivity frequency distribution curve presented a single peak in a narrow range. No cross‐resistance was found between the DMI fungicide metconazole and the benzimidazole fungicide carbendazim or the cyanoacrylate fungicide phenamacril. Therefore, these sensitivity data could be used as the baseline of F. graminearum susceptibility to metconazole in the Henan Province and provide the basis for monitoring metconazole resistance in this area.

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“…A novel cyanoacrylate fungicide called phenamacril (experimental code JS399‐19) was developed by the Jiangsu Branch of National Pesticide Research and Development Center of China in 1998 and showed specific antifungal activity towards Fusarium spp. Since 2007, it has been widely used in China for controlling FHB and rice bakanae disease caused by F. fujikuroi . Previous studies revealed that the class I myosin, FgMyoI is the drug target of phenamacril in F. graminearum .…”

Section: Introductionmentioning

confidence: 99%

“…Phenamacril directly binds with FgMyo1 and suppresses the ATPase activity of FgMyo1 . In the laboratory, substitution of an amino acid residue in FgMyo1 or its homologs in F. asiaticum and F. fujikuroi leads to high resistance to the fungicide . Until now, phenamacril resistant strains of F. graminearum have not been found in the field, while resistant strains of F. fujikuroi have existed in the field for some time .…”

Section: Introductionmentioning

confidence: 99%

The b‐ZIP transcription factor FgTfmI is required for the fungicide phenamacril tolerance and pathogenecity in Fusarium graminearum

Liu

Dawood

et al. 2019

Pest Management Science

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BACKGROUND Fusarium head blight (FHB) is a devastating disease of cereal crops worldwide mainly caused by Fusarium graminearum. Due to the unavailability of FHB‐resistant wheat cultivars, chemical fungicide application is currently the most effective approach for controlling FHB now. In the last few years, a novel cyanoacrylate fungicide, phenamacril, has been widely used in China for FHB disease management. In previous studies, we identified that myosin I (FgMyo1) is the target of phenamacril and is essential for mycotoxin deoxynivalenol (DON) biosynthesis and fungal growth. However, the regulation of FgMYO1 gene expression is still largely unknown. RESULTS In this study, we identified a b‐ZIP transcription factor, FgTfmI, which regulates the mRNA expression of FgMYO1 upon phenamacril treatment. The FgTfmI directly binds to the promoter region of FgMYO1, and is required for the upregulation of FgMYO1 in response to phenamacril treatment. The deletion mutant of FgTFMI (ΔFgTfmI) displayed a slight growth defect, while it showed hypersensitivity to phenamacril, but not to other tested fungicides. FgTfmI also contributed to DON biosynthesis and the infection process in planta. CONCLUSIONS The transcription factor FgTfmI plays an important role in regulating transcription of the genes involved in phenamacril tolerance, DON biosynthesis and virulence in F. graminearum. © 2019 Society of Chemical Industry

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Resistance mechanism of Fusarium fujikuroi to phenamacril in the field

Abstract: In the field, the point mutation S219P or S219 L in Myosin-5 conferred high resistance to phenamacril in F. fujikuroi. The point mutation S219P did not affect the fitness of F. fujikuroi, while the point mutation S219 L decreased its fitness. © 2017 Society of Chemical Industry.

Cited by 67 publications

References 24 publications

Resistance risk assessment for Fusarium graminearum to pydiflumetofen, a new succinate dehydrogenase inhibitor

Resistance risk assessment for Fusarium graminearum to pydiflumetofen, a new succinate dehydrogenase inhibitor

Baseline sensitivity of Fusarium graminearum from wheat fields in Henan, China, to metconazole and analysis of cross resistance with carbendazim and phenamacril

The b‐ZIP transcription factor FgTfmI is required for the fungicide phenamacril tolerance and pathogenecity in Fusarium graminearum

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