Resistance to heat and cold stress in Drosophila melanogaster: intra and inter population variation in relation to climate

Guerra, Daniela; Cavicchi, Sandro; Krebs, RA; Loeschcke, Volker

doi:10.1186/1297-9686-29-4-497

Cited by 31 publications

(14 citation statements)

References 5 publications

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“…Comparisons of thermal tolerance of lab-bred flies derived from distinct geographic locations, however, have produced conflicting results. In some cases, geographic comparisons of thermal tolerance traits indicate that differences among populations are consistent with what is predicted by local environmental thermal regimes (Krebs and Loeschcke, 1995;Guerra et al, 1997;Sorensen et al, 2001). Other comparisons where latitudinal variation in thermal tolerance is expected have failed to reveal such differences (Davidson, 1990;Kimura et al, 1994).…”

Section: Introductionmentioning

confidence: 83%

Intraspecific variation in thermal tolerance and heat shock protein gene expression in common killifish,Fundulus heteroclitus

Fangue

Hofmeister

Schulte

2006

Journal of Experimental Biology

431

384

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SUMMARY Populations of common killifish, Fundulus heteroclitus, are distributed along the Atlantic coast of North America through a steep latitudinal thermal gradient. We examined intraspecific variation in whole-animal thermal tolerance and its relationship to the heat shock response in killifish from the northern and southern extremes of the species range. Critical thermal maxima were significantly higher in southern than in northern fish by ∼1.5°C at a wide range of acclimation temperatures (from 2-34°C), and critical thermal minima differed by ∼1.5°C at acclimation temperatures above 22°C, converging on the freezing point of brackish water at lower acclimation temperatures. To determine whether these differences in whole-organism thermal tolerance were reflected in differences in either the sequence or regulation of the heat shock protein genes(hsps) we obtained complete cDNA sequences for hsc70, hsp70-1 and hsp70-2, and partial sequences of hsp90α and hsp90β. There were no fixed differences in amino acid sequence between populations in either hsp70-1 or hsp70-2, and only a single conservative substitution between populations in hsc70. By contrast, there were significant differences between populations in the expression of many, but not all, of these genes. Both northern and southern killifish significantly increased hsp70-2 levels above control values(Ton) at a heat shock temperature of 33°C, but the magnitude of this induction was greater in northern fish, suggesting that northern fish may be more susceptible to thermal damage than are southern fish. In contrast, hsp70-1 mRNA levels increased gradually and to the same extent in response to heat shock in both populations. Hsc70 mRNA levels were significantly elevated by heat shock in southern fish, but not in northern fish. Similarly, the more thermotolerant southern killifish had a Ton for hsp90α of 30°C, 2°C lower than that of northern fish. This observation combined with the ability of southern killifish to upregulate hsc70 in response to heat shock suggests a possible role for these hsps in whole-organism differences in thermal tolerance. These data highlight the importance of considering the complexity of the heat shock response across multiple isoforms when attempting to make linkages to whole-organism traits such as thermal tolerance.

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Section: Introductionmentioning

confidence: 83%

Intraspecific variation in thermal tolerance and heat shock protein gene expression in common killifish,Fundulus heteroclitus

Fangue

Hofmeister

Schulte

2006

Journal of Experimental Biology

431

384

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“…Research in Drosophila is providing a focus for understanding the physiological and genetic basis of thermal tolerance variation in ectotherms (Huey and Kingsolver, 1993;Duncker et al, 1995;Krebs and Loeschcke, 1995;Guerra et al, 1997;Ohtsu et al, 1998). Significant variation in resistance to temperature extremes are known among several groups of closely related Drosophila species and these are likely to be adaptive since they are related to the climatic zones where the species are found (Kimura, 1988;Jenkins and Hoffmann, 1994;Gibert et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Thermal tolerance trade-offs associated with the right arm of chromosome 3 and marked by the hsr-omega gene in Drosophila melanogaster

et al. 2003

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Drosophila melanogaster occurs in diverse climatic regions and shows opposing clinal changes in resistance to heat and resistance to cold along a 3000 km latitudinal transect on the eastern coast of Australia. We report here on variation at a polymorphic 8 bp-indel site in the heat shock hsr-omega gene that maps to the right arm of chromosome 3. The frequency of the genetic element marked by the L form of the gene was strongly and positively associated with latitude along this transect, and latitudinal differences in L frequency were robustly associated with latitudinal differences in maximum temperature for the hottest month. On a genetic background mixed for genes from each end of the cline a set of 10 lines was derived, five of which were fixed for the L marker, the absence of In(3R)P and 12 kb of repeats at a second polymorphic site at the 3 0 end of hsr-omega, and five that were fixed for the S marker, In(3R)P and 15 kb of hsromega repeats. For two different measures of heat tolerance S lines outperformed L lines, and for two different measures of cold tolerance L lines outperformed S lines. These data suggest that an element on the right arm of chromosome 3, possibly In(3R)P, confers heat resistance but carries the trade-off of also conferring susceptibility to cold. This element occurs at high frequency near the equator. The alternate element on the other hand, at high frequency at temperate latitudes, confers cold resistance at the cost of heat susceptibility.

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“…Drosophila species have been of considerable focus in attempts to elucidate such mechanisms because, apart from the obvious benefits of their experimental and genomic tractability, there are numerous examples of closely related species with quite different thermal tolerance attributes and of intra-specific ecotype variation where strains from warm tropical regions at low latitudes are more heat tolerant and/or more cold sensitive than those from cool temperate higher latitudes (Kimura et al, 1994;Guerra et al, 1997;Hoffmann et al, 2002). Heritable, climatically-associated variation in stress resistance traits provide the opportunity to investigate and understand the potential of and limits to the processes of adaptation to thermal extremes.…”

Section: Introductionmentioning

confidence: 99%

Latitudinal and cold-tolerance variation associate with DNA repeat-number variation in the hsr-omega RNA gene of Drosophila melanogaster

et al. 2008

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Resistance to heat and cold stress in Drosophila melanogaster: intra and inter population variation in relation to climate

Cited by 31 publications

References 5 publications

Intraspecific variation in thermal tolerance and heat shock protein gene expression in common killifish,Fundulus heteroclitus

Intraspecific variation in thermal tolerance and heat shock protein gene expression in common killifish,Fundulus heteroclitus

Thermal tolerance trade-offs associated with the right arm of chromosome 3 and marked by the hsr-omega gene in Drosophila melanogaster

Latitudinal and cold-tolerance variation associate with DNA repeat-number variation in the hsr-omega RNA gene of Drosophila melanogaster

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