Resistance to the chemotherapeutic agents of Staphylococcus aureus strains isolated from hospitalized patients

Junie, Lia Monica; Simon, Laura Mihaela; Pandrea, Stanca Lucia

doi:10.1016/j.ijid.2014.03.593

Cited by 4 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S. aureus is also a leading cause of hospital-associated (HA) and community-associated (CA) bacterial infections in humans, associating with numerous mild skin and soft tissue infections, as well as life-threatening pneumonia, bacteraemia, osteomyelitis, endocarditis, sepsis and toxic shock syndrome (David & Daum, 2010). The increasing prevalence of meticillinresistant S. aureus (MRSA) and its ability to resist multiple drugs has posed a serious challenge for infection control (Junie et al, 2014). HA-MRSA often infects individuals with health care risk factors, such as surgery or residence in a long-term care facility.…”

Section: Introductionmentioning

confidence: 99%

Staphylococcus aureus ST121: a globally disseminated hypervirulent clone

Rao

Shang

et al. 2015

Journal of Medical Microbiology

View full text Add to dashboard Cite

Staphylococcus aureus is a leading cause of bacterial infections in hospitals and communities worldwide. With the development of typing methods, several pandemic clones have been well characterized, including the extensively spreading hospital-associated meticillin-resistant S. aureus (HA-MRSA) clone ST239 and the emerging hypervirulent community-associated (CA) MRSA clone USA300. The multilocus sequence typing method was set up based on seven housekeeping genes; S. aureus groups were defined by the sharing of alleles at $5 of the seven loci. In many cases, the predicted founder of a group would also be the most prevalent ST within the group. As a predicted founder of major S. aureus groups, approximately 90 % of ST121 strains was meticillin-susceptible S. aureus (MSSA). The majority of ST121 strains carry accessory gene regulator type IV, whereas staphylococcal protein A gene types for ST121 are exceptionally diverse. More than 90 % of S. aureus ST121 strains have Panton-Valentine leukocidin; other enterotoxins, haemolysins, leukocidins and exfoliative toxins also contribute to the high virulence of ST121 strains. Patients suffering from S. aureus ST121 infections often need longer hospitalization and prolonged antimicrobial therapy. In this review, we tried to summarize the epidemiology of the S. aureus clone ST121 and focused on the molecular types, toxin carriage and disease spectrum of this globally disseminated clone.

show abstract

Section: Introductionmentioning

confidence: 99%

Staphylococcus aureus ST121: a globally disseminated hypervirulent clone

Rao

Shang

et al. 2015

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…Proper surveillance of the medical staff and a rational policy in prescribing antibiotics in these hospitals are therefore mandatory. Controlled trials must determine the potential medical and economic benefit of control strategies using this technology in hospital care units [27].…”

Section: Discussionmentioning

confidence: 99%

The Phenotypic and Genetic Detection of the Methicillin-Resistant Staphylococcus aureus (MRSA)

Eftychios¹,

Vm²,

Calina-Oana³

et al. 2020

J Emerg Dis Virol

View full text Add to dashboard Cite

Methicillin resistant strains of Staphylococcus aureus (MRSA) were identified shortly upon the introduction of methicillin into the clinical practice. The S. aureus samples were taken from patients hospitalized in General Hospital of Chania “Agios Georgios”. The strains were isolated from different pathological products, in the hospital laboratory. All isolates were tested using the cefoxitin disk diffusion, the oxacillin MIC methods and the PBP2’ latex agglutination test (bioMérieux), for the production of the Penicillin-Binding Protein 2a (PBP2a or PBP2’ protein). S. aureus ATCC 29213 was used as a negative control. We followed the detection of the mecA gene throughout the PCR method, as the standard “gold” method, in order to identify MRSA strains. Conventional methods for MRSA strains detection were compared with the PCR method. The antibiotic susceptibility testing was performed throughout the Kirby-Bauer disk diffusion method using antibiotic discs from Bioanalyse ltd. The mecA gene was found by PCR in 57.5 % of S. aureus strains, which allowed defining the isolated strains as MRSA strains. According to the oxacillin MIC values of the studied strains, 25 strains (53.2%) were identified as MRSA, 21 (44.68%) as MSSA and 1*strain (2.13%) as Borderline (BL) MRSA. The mecA gene is present in 24 of the MRSA strains with oxacillin MIC ≥ 4 being more common in strains showing the oxacillin MICs ≥ 256 (12/27). Adding the BL strains to the methicillin resistant strains, the rate of the MRSA strains increases to 26 (55.32%), the appropriate values of the MRSA strains percent, as determined by the PCR method (57.45%), which shows a concordance of 96.3% (26/27), between the results obtained by the two tests. Comparing the results obtained using the PCR method; with the oxacillin MICs and the PBP2’ latex agglutination test, concordant results were obtained for 89.36% of the strains (42/47) by oxacillin MICs and for 97.87% of the strains (46/47) by PBP2’ latex agglutination test. We conclude that the specificity of these methods is 100% for the mecA PCR method, 97.87% for the PBP2a latex method and 89.36% for the oxacillin MIC. The comparison of phenotypic methods (the PBP2a latex reaction, oxacillin MICs, the Cefoxitin disk diffusion test with the genotypic methods (the presence of the mecA gene), reveals that the PBP2a latex reaction has high sensitivity (97.87%), and can be used as an alternative method to PCR for the MRSA detection, in resource constraint settings.

show abstract

“…The increasing prevalence of methicillin-resistant S. aureus (MRSA) and its ability to resist multiple drugs has posed a serious challenge to infection control. 1,2 Escherichia coli is one of the earliest colonizers of the gastrointestinal tract; although eventually, it is a minor component of the colonic gut microbiome in humans, where it represents less than 0.1% of the total bacterial cells. Nevertheless, due to the overall high cell density in the colon, this small percentage translates into around 108 cells/ml.…”

Section: Introductionmentioning

confidence: 99%

A potential role of extracellular DNA in biofilm and ciprofloxacin resistance

Ibrahim¹,

Mussa

Al-Mathkhury

2023

J. Contemp. Med. Sci.

View full text Add to dashboard Cite

Objectives: This study aims to broaden our knowledge of the role of eDNA in bacterial biofilms and antibiotic-resistance gene transfer among isolates. Methods: Staphylococcus aureus, E. coli, and Pseudomonas aeruginosa were isolated from different non-repeated 170 specimens. The bacterial isolates were identified using morphological and molecular methods. Different concentrations of genomic DNA were tested for their potential role in biofilms formed by study isolates employing microtiter plate assay. Ciprofloxacin resistance was identified by detecting a mutation in gyrA and parC. Results: The biofilm intensity significantly decreased (P < 0.05) concerning S. aureus isolates and insignificantly (P > 0.05) concerning E. coli isolates. Yet, one E. coli isolate's biofilm was significantly decreased (P < 0.05) linearly with increasing eDNA. Of considerable interest, the addition of eDNA led to a significant increase (P < 0.05) in the biofilm of the two-tested P. aeruginosa isolates. Moreover, eDNA participated in transferring Ciprofloxacin resistance to the sensitive isolate when it presents in its biofilm. Conclusion: eDNA has a dual effect on bacterial biofilms either supportive or suppressive following bacterial species per se. Also, it seems to play an important role in antibiotic resistance within the biofilm.

show abstract

Resistance to the chemotherapeutic agents of Staphylococcus aureus strains isolated from hospitalized patients

Cited by 4 publications

References 0 publications

Staphylococcus aureus ST121: a globally disseminated hypervirulent clone

Staphylococcus aureus ST121: a globally disseminated hypervirulent clone

The Phenotypic and Genetic Detection of the Methicillin-Resistant Staphylococcus aureus (MRSA)

A potential role of extracellular DNA in biofilm and ciprofloxacin resistance

Contact Info

Product

Resources

About