Resistance to Tuber Moth Following the Transfer to Potato of a Cry9aa2 Gene Under the Control of Constitutive and Light-Inducible Promoters

Meiyalaghan, Sathiyamoorthy; Takla, Michael; Barrell, Philippa J.; Keijzer, Rosalinde M.; Jacobs, Jeanne M. E.; Conner, A. J.

doi:10.17660/actahortic.2005.670.7

Cited by 5 publications

(15 citation statements)

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“…4). However, this level of resistance was no greater than that previously observed from the use of only the cry1Ac9 gene (Davidson et al 2002, 2004, Meiyalaghan et al 2006a or only the cry9Aa2 gene (Meiyalaghan et al 2005(Meiyalaghan et al , 2006aJacobs et al 2009). The failure to observe an enhanced level of resistance to PTM larvae is surprising given the previous experimental results from simulating the pyramiding of several cry genes (Meiyalaghan et al 2006c).…”

Section: Re-transformation Simultaneous Transformationcontrasting

confidence: 53%

“…The I75 line was transgenic for 35S-cry1Ac9-ocs and nos-nptII-nos chimeric genes (Davidson et al 2002), whereas the DG4c line was transgenic for 35S-cry9Aa2-ocs and nos-nptII-nos chimeric genes (Meiyalaghan et al 2005). Both I75 and DG4c produced phenotypically normal plants with high resistance to PTM (Davidson et al 2002;Meiyalaghan et al 2005).…”

Section: Plant Materialsmentioning

confidence: 99%

“…The qRT-PCR data for each sample were normalized to the amount of ef1a transcript using the same amplification conditions. The relative abundance of each cry gene transcript in transgenic plants was determined using the DDC t method relative to the I75 and DG4c lines, which were used as positive controls that are known to have high resistance to PTM larvae from the expression of the cry1Ac9 and cry9Aa2 genes, respectively (Davidson et al 2002;Meiyalaghan et al 2005).…”

Section: Qualitative Reverse Transcription (Rt)-pcr Analysesmentioning

confidence: 99%

“…Mean growth indices (Davidson et al 2002;Meiyalaghan et al 2005). The standard error of each mean is represented by the vertical line is inappropriate in clonal crops such as potatoes.…”

Section: Re-transformation Simultaneous Transformationmentioning

confidence: 99%

“…However, with the advent of genetic engineering, genes for insect resistance can be transferred into plants to develop insectresistant plants. Transgenic potatoes that express cry genes derived from various strains of the soil bacterium Bacillus thuringiensis (Bt) have exhibited resistance to PTM (Jansens et al 1995;Cañedo et al 1999;Li et al 1999;Chakrabarti et al 2000, Douches et al 2002Davidson et al 2002Davidson et al , 2004Meiyalaghan et al 2005Meiyalaghan et al , 2006aHagh et al 2009;Jacobs et al 2009;Kumar et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Pyramiding transgenes for potato tuber moth resistance in potato

Meiyalaghan

Pringle

Barrell

et al. 2010

Plant Biotechnol Rep

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The feasibility of two strategies for transgene pyramiding using Agrobacterium-mediated transformation was investigated to develop a transgenic potato (Solanum tuberosum L. cv. Iwa) with resistance to potato tuber moth (PTM) (Phthorimaea operculella (Zeller)). In the first approach, cry1Ac9 and cry9Aa2 genes were introduced simultaneously using a kanamycin (nptII) selectable marker gene. The second approach involved the sequential introduction (re-transformation) of a cry1Ac9 gene, using a hygromycin resistance (hpt) selectable marker gene, into an existing line transgenic for a cry9Aa2 gene and a kanamycin resistance (nptII) selectable marker gene. Multiplex polymerase chain reaction (PCR) confirmed the presence of the specific selectable marker gene and both cry genes in all regenerated lines. The relative steady-state level of the cry gene transcripts in leaves was quantified in all regenerated lines by real-time PCR analysis. Re-transformation proved to be a flexible approach to effectively pyramid genes for PTM resistance in potato, since it allowed the second gene to be added to a line that was previously identified as having a high level of resistance. Larval growth of PTM was significantly inhibited on excised greenhouse-grown leaves in all transgenic lines, although no lines expressing both cry genes exhibited any greater resistance to PTM larvae over that previously observed for the individual genes. It is anticipated that these lines will permit more durable resistance by delaying the opportunities for PTM adaptation to the individual cry genes.

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Section: Re-transformation Simultaneous Transformationcontrasting

confidence: 53%

Section: Plant Materialsmentioning

confidence: 99%

Section: Qualitative Reverse Transcription (Rt)-pcr Analysesmentioning

confidence: 99%

“…Mean growth indices (Davidson et al 2002;Meiyalaghan et al 2005). The standard error of each mean is represented by the vertical line is inappropriate in clonal crops such as potatoes.…”

Section: Re-transformation Simultaneous Transformationmentioning

confidence: 99%