RESISTÊNCIA AOS ANTIMICROBIANOS EM Staphylococcus spp. ASSOCIADOS À MASTITE BOVINA

Moritz, Fábio; Moritz, Cristiane Mengue Feniman

doi:10.4025/revcivet.v3i2.34435

Cited by 8 publications

(9 citation statements)

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“…Mastitis is a frequent cause of economic losses in dairy cows, compromising animal health, milk composition and public health. The incorrect and indiscriminate antimicrobial use in animals has selected multiresistant bacteria (Moritz and Moritz, 2016), as evidenced for Staphylococcus spp. strains isolated from cows (Liu et al., 2017; Unakal and Kaliwal, 2010; Wang et al., 2013).…”

Section: Introductionmentioning

confidence: 99%

Extract of Mangifera indica L. leaves may reduce biofilms of Staphylococcus spp. in stainless steel and teatcup rubbers

Manzur

Valdo

Morais-Costa

et al. 2019

Food sci. technol. int.

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Human ingestion of antimicrobial residues in dairy products is a problem of public health. In this study we evaluated antibacterial effectiveness of aqueous and ethanolic leaf extracts of Mangifera indica L. as a natural alternative for reduction of Staphylococcus spp. biofilms. Eight Staphylococcus spp. strains from cows with mastitis and the Staphylococcus aureus strain (ATCC 25923) were evaluated. High performance liquid chromatography indicated the presence of phenolic compounds in the aqueous extract. Agilent Technologies gas chromatography revealed the presence of phenolic compounds, carbohydrates, and gallic acid in ethanolic extract. The tannin contents were 2.27 ± 0.01 and 2.67 ± 0.01% for ethanolic extract and aqueous extract, respectively. Extracts were evaluated in agar diffusion test and the size of inhibition zones were compared. The ethanolic extract was the most effective, showing minimum inhibitory concentrations and minimum bactericidal concentrations between 1.8–7.5 and 15.1–45.3 mg/ml, respectively. After tannin removal, the extracts were less effective, indicating these secondary metabolites as one of the main antibacterial compounds. The ethanolic extract at 45.3 mg/ml reduced mature biofilms in teat rubber (average: 3.91 log reduction) and stainless steel (average: 3.87 log reduction) after 5 min of contact. The extracts of M. indica leaves represent natural alternatives against Staphylococcus spp. strains and the ethanolic extract shows potential as a natural sanitizer.

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Section: Introductionmentioning

confidence: 99%

Extract of Mangifera indica L. leaves may reduce biofilms of Staphylococcus spp. in stainless steel and teatcup rubbers

Manzur

Valdo

Morais-Costa

et al. 2019

Food sci. technol. int.

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“…(Silva et al, 2013). Um método eficaz e de baixo custo para o controle e prevenção da mastite é o uso de boas práticas durante a ordenha, entretanto, na conduta terapêutica é importante a identificação do patógeno e o monitoramento da resistência aos antimicrobianos nos casos de mastite subclínica (Moritz;Moritz 2016).…”

Section: Introductionunclassified

“…O uso inadequado e indiscriminado de antimicrobianos tem promovido a ocorrência de cepas resistentes, prejudicando a eficiência do tratamento, tornando-se um problema de saúde humana e animal (Mendonça et al, 2012); Moritz;Moritz (2016). Diversos microrganismos patogênicos podem causar inflamação no teto do animal, entretanto Staphylococcus aureus (S. aureus) é um dos principais agentes etiológicos causadores da mastite bovina (Silva et al, 2013;Song et al, 2016;Sheet et al, 2019).…”

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Caracterização genotípica de Staphylococcus aureus resistentes à meticilina isolados de tetos e leite bovino

et al. 2020

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O presente estudo teve como objetivo verificar a presença dos genes mecA, mecA LGA251, blaZ em isolados de S. aureus resistente a antibióticos beta-lactâmicos, provenientes de amostras de vacas diagnosticadas com mastite subclínica de rebanhos do norte de Minas Gerais. Isolados previamente identificados através da detecção do gene femA e MALDI-TOF MS e resistentes a antibióticos do grupo beta-lactâmicos foram utilizados. Os isolados foram submetidos à extração de DNA e para o rastreamento dos genes de resistência utilizou se a técnica de PCR. Embora fenotipicamente resistente a oxacilina e cefoxitina nenhuma das linhagens apresentou resultado positivo para os genes mecA e mecALGA251 nos ensaios efetuados, a ausência destes não descarta a possibilidade da presença de outros genes de resistências nas propriedades rurais em estudo. Porém, a presença do gene blaZ foi verificada em 4 (12,90%) dos isolados em estudo.Os resultados destacam a necessidade do monitoramento e adoção de medidas de controle que evitem à disseminação desses genes que representam grande risco a saúde pública.

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“…Mastitis treatment and prevention consists mainly of the use of antibiotics and proper animal handling to prevent the spread of the disease to healthy animals. However, the use of antibiotics requires caution in order to avoid the emergence of resistant bacteria (Costa et al, ; Moritz & Moritz, ). Krewer et al () found simultaneous resistance to three or more antibiotics in 65.6% of Staphylococcus isolates that cause mastitis.…”

Section: Introductionmentioning

confidence: 99%

Looking for alternative treatments for bovine and caprine mastitis: Evaluation of the potential of Calliandra surinamensis leaf pinnulae lectin (CasuL), both alone and in combination with antibiotics

et al. 2019

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This work aimed to evaluate the effects of CasuL on growth and viability of 15 mastitis isolates from cows and goats, to determine the synergistic potential between CasuL and antibiotics, and to investigate the effects on bacterial ultrastructure and antibiofilm activity. The lectin inhibited the growth of Staphylococcus isolates from either bovine (Ssp6PD and Sa) or caprine (Ssp5D and Ssp01) mastitis. The minimal inhibitory concentrations were ranged from 3.75 to 15 µg/ml. Synergistic effect was observed for CasuL‐tetracycline against Sa and Ssp6PD and CasuL‐ampicillin against Ssp01. No structural damage was observed under the scanning electron microscope in CasuL treatments. Flow cytometry analysis using thiazol orange and propidium iodide demonstrated that CasuL was unable to reduce the cell viability of the isolates tested. At sub‐inhibitory concentrations, CasuL reduced biofilm formation by the isolates Sa and Ssp5D. However, CasuL‐tetracycline and CasuL‐ampicillin combinations inhibited biofilm formation by Ssp6PD and Ssp01, respectively. In conclusion, CasuL is a bacteriostatic and antibiofilm agent against some mastitis isolates and displayed a synergistic potential when used in combination with either ampicillin (against one isolate) or tetracycline (against two isolates). The results stimulate the evaluation of CasuL for the treatment of mastitis, particularly when used in conjunction with antibiotics.

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RESISTÊNCIA AOS ANTIMICROBIANOS EM Staphylococcus spp. ASSOCIADOS À MASTITE BOVINA

Cited by 8 publications

References 0 publications

Extract of Mangifera indica L. leaves may reduce biofilms of Staphylococcus spp. in stainless steel and teatcup rubbers

Extract of Mangifera indica L. leaves may reduce biofilms of Staphylococcus spp. in stainless steel and teatcup rubbers

Caracterização genotípica de Staphylococcus aureus resistentes à meticilina isolados de tetos e leite bovino

Looking for alternative treatments for bovine and caprine mastitis: Evaluation of the potential of Calliandra surinamensis leaf pinnulae lectin (CasuL), both alone and in combination with antibiotics

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