We propose a mechanism to directly measure the chiral anomaly in disorder Weyl semimetals (WSMs) by the Kondo effect. We find that in a magnetic and electric field driven WSM, the locations of the Kondo peaks can be modulated by the chiral chemical potential, which is proportional to E • B. The Kondo peaks come from spin fluctuations within the impurities, which apart from the temperature, relate closely to the host's Fermi level. In WSMs, the chiral-anomaly-induced chirality population imbalance will shift the local Fermi levels of the paired Weyl valleys toward opposite directions in energy, and then affects the Kondo effect. Consequently, the Kondo effect can be tunable by an external electric field via the chiral chemical potential. This is unique to the chiral anomaly. Base on this, we argue that the electrically tunable Kondo effect can serve as a direct measurement of the chiral anomaly in WSMs. The Kondo peaks are robust against the disorder effect and therefore, the signal of the chiral anomaly survives for a relatively weak magnetic field.