Respiration of the Cornea

deROETTH, Andrew

doi:10.1001/archopht.1950.00910020678005

Cited by 35 publications

(3 citation statements)

References 7 publications

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“…Particularly, this metabolic pathway is prevalent especially in corneal tissue (de Roetth, 1950;Chen and Chen, 1990) since about 70 % of the glucose is metabolized through glycolysis (Kinoshita, Masurat and Helfant, 1955;Aguayo et al, 1988, Chen andChen, 1990). Moreover, it was shown that LDH was highly active in rat, cat and, particularly, in rabbit corneal epithelium (Kuhlman, 1959).…”

Section: Discussionmentioning

confidence: 99%

Differentiation-dependent Increases in Lactate Dehydrogenase Activity and Isoenzyme Expression in Rabbit Corneal Epithelial Cells

Hernández-Quintero

García-Villegas

Castro‐Muñozledo

2002

Experimental Eye Research

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Section: Discussionmentioning

confidence: 99%

Differentiation-dependent Increases in Lactate Dehydrogenase Activity and Isoenzyme Expression in Rabbit Corneal Epithelial Cells

Hernández-Quintero

García-Villegas

Castro‐Muñozledo

2002

Experimental Eye Research

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“…To determine the rate of acid production aerobically it has been necessary to assume an R.Q. of unity (Duane, 1949;deRoetth, 1950). Direct manometric determination of the rate of acid production in the stroma was made after removal of the epithelial and endothelial cells by scraping.…”

Section: Methodsmentioning

confidence: 99%

“…In contrast, when respiration was maximal the amount of lactic acid accumulated accounted for only half the total acid, assuming that the R.Q. of this tissue is unity (Duane, 1949;deRoetth, 1950). The rate of accumulation of lactic acid in thefreshly excised cornea.…”

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confidence: 99%

Glycolysis in the cornea of the rabbit

Langham¹

1954

The Journal of Physiology

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Numerous investigators have observed that the excised cornea respires and that glycolysis takes place in both aerobic and anaerobic conditions. In particular, Kohra (1935), Fischer (1940) and deRoetth (1951) observed that in the absence of oxygen the rate of glycolysis increased in all corneal layersthe epithelium, the stroma and the endothelium. Further, they demonstrated that the collagenous stroma, comprising nine-tenths of the corneal mass, possessed a very low metabolic activity compared to the outer cellular layers. Kohra studied rabbits, deRoetth cattle, while Fischer did not state the species employed. All these authors used a manometric technique to determine glycolytic rates, and deRoetth in addition made simultaneous analyses of the rate of accumulation of lactic acid in the cornea using a chemical technique. His findings differed from earlier workers in one important respect in that he observed no glycolysis or lactic acid accumulation in the epithelium in the presence of oxygen.An absence of lactic acid production in the epithelium in aerobic conditions is not readily reconciled with the observation that lactic acid exists in this layer in a concentration exceeding that in the stroma both in bovine and rabbit cornea (Herrman & Hickman, 1948;Langham, 1952). In an attempt to elucidate this problem the rate of glycolysis in the whole excised cornea of the rabbit has been determined and related to the rate of accumulation of lactic acid in aerobic and anaerobic conditions, and to the rate of glycolysis in the component layers determined by a manometric technique.The supply of oxygen to the cornea presents special problems, and it is still uncertain whether the tissue can obtain sufficient to respire at a maximal rate. In an earlier investigation (Langham, 1952) it was observed that the concentration of lactic acid in the cornea of the rabbit decreased when the atmospheric oxygen tension was raised. The possibility that this may reflect a change in the rate of accumulation of lactic acid has now been investigated by comparing the rates at which lactic acid accumulates in the cornea of the living animal and in the excised tissue.

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Glycolytic Activity of the Cornea

deROETTH¹

1951

Archives of Ophthalmology

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Respiration of the Cornea

Cited by 35 publications

References 7 publications

Differentiation-dependent Increases in Lactate Dehydrogenase Activity and Isoenzyme Expression in Rabbit Corneal Epithelial Cells

Differentiation-dependent Increases in Lactate Dehydrogenase Activity and Isoenzyme Expression in Rabbit Corneal Epithelial Cells

Glycolysis in the cornea of the rabbit

Glycolytic Activity of the Cornea

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