Response Surface Methodology as a Useful Tool for Development and Optimization of Sustained Release Ketorolac Tromethamine Niosomal Organogels

Elsayed, Mahmoud M. A.; Rasoul, Saleh Abd El; Ramadan, Abd El hakim; Hussein, Amal K.

doi:10.1007/s12247-019-09421-4

Cited by 13 publications

(13 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Resultsmentioning

confidence: 99%

“…Fifteen formulae of 5-FuCrNPs were prepared using Box–Behnken Design (BBD) [ 35 , 36 ]. Three independent variables were chosen: 5-Fu concentration (0.25, 0.5 and 0.75%), the weight of CrNPs (250, 500 and 750 mg), and reflux temperature in the water bath shaker (20 ± 1 °C, 25 ± 1 °C, and 30 ± 1 °C).…”

Section: Methodsmentioning

confidence: 99%

“…The LE% depends on the 5-Fu concentration, CrNP weight and temperature, and ranges from 55.87 ± 2.03% to 92.11 ± 3.23%. The regression Equation (7) of the fitted LE% model is [36,42]: LE% = 79.45 + 7.21 X 1 + 6.45 X 2 − 4.99 X 3 − 1.19 X 1 2 − 5.24 X 2 2 + 2.25 X 3 2 − 1.31 X 1 X 2 + 0.57 X 1 X 3 − 0.14 X 2 X 3 (7) Since the p-value in the ANOVA table is less than 0.1, there is a statistically significant relationship between the variables at the 90% and higher confidence level. The R-squared statistic indicates that the model (as fitted) explains 36.23% of the variability in 5-Fu concentration.…”

Section: Drug Loading Efficiency On Crnps (Le%)mentioning

confidence: 99%

See 2 more Smart Citations

Novel Green Biosynthesis of 5-Fluorouracil Chromium Nanoparticles Using Harpullia pendula Extract for Treatment of Colorectal Cancer

et al. 2021

Self Cite

View full text Add to dashboard Cite

Colorectal cancer (CRC) is the third highest major cause of morbidity and mortality worldwide. Hence, many strategies and approaches have been widely developed for cancer treatment. This work prepared and evaluated the antitumor activity of 5-Fluorouracil (5-Fu) loaded chromium nanoparticles (5-FuCrNPs). The green biosynthesis approach using Harpullia (H) pendula aqueous extract was used for CrNPs preparation, which was further loaded with 5-Fu. The prepared NPs were characterized for morphology using scanning and transmission electron microscopes (SEM and TEM). The results revealed the formation of uniform, mono-dispersive, and highly stable CrNPs with a mean size of 23 nm. Encapsulation of 5-Fu over CrNPs, with a higher drug loading efficiency, was successful with a mean size of 29 nm being produced. In addition, Fourier transform infrared (FTIR) and X-ray diffraction pattern (XRD) were also used for the investigation. The drug 5-Fu was adsorbed on the surface of biosynthesized CrNPs in order to overcome its clinical resistance and increase its activity against CRC cells. Box–Behnken Design (BBD) and response surface methodology (RSM) were used to characterize and optimize the formulation factors (5-Fu concentration, CrNP weight, and temperature). Furthermore, the antitumor activity of the prepared 5-FuCrNPs was tested against CRC cells (CACO-2). This in vitro antitumor study demonstrated that 5-Fu-loaded CrNPs markedly decreased the IC50 of 5-Fu and exerted more cytotoxicity at nearly all concentrations than 5-Fu alone. In conclusion, 5-FuCrNPs is a promising drug delivery system for the effective treatment of CRC.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Drug Loading Efficiency On Crnps (Le%)mentioning

confidence: 99%

See 1 more Smart Citation

Novel Green Biosynthesis of 5-Fluorouracil Chromium Nanoparticles Using Harpullia pendula Extract for Treatment of Colorectal Cancer

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…The ultracentrifugation method was used to characterize the EE% of LUT-ENPs using the Biofuge Centrifuge (Primo Heraeus, Hanau, Germany) at 10,000 rpm for 60 min [ 19 , 21 ]. PBS, pH 7.4 was used to dilute 1 mL of the supernatant obtained and measured at 356 nm using PBS, pH 7.4 as a blank.…”

Section: Methodsmentioning

confidence: 99%

“…LUT-ENPs Size and Polydispersity Index (PDI) For the optimization of different formulation variables three levels of each independent variable were used. The selected levels of EtOH concentrations (X 1 ) were 15(−1), 30(0), and 45% (1) (v/v), whereas the selected levels of SL concentrations were (X 2 ) 2(−1), 4(0), and 6(1) %(w/v) (Table 1) [19,20].…”

Section: Morphology Of the Prepared Lut-enpsmentioning

confidence: 99%

Design and Optimization of Orally Administered Luteolin Nanoethosomes to Enhance Its Anti-Tumor Activity against Hepatocellular Carcinoma

et al. 2021

Self Cite

View full text Add to dashboard Cite

Luteolin (LUT) is a natural flavonoid with low oral bioavailability with restricted clinical applications due to its low solubility. LUT shows significant anti-tumor activity in many cancer cells, including hepatocellular carcinoma (HCC). The most recent trend in pharmaceutical innovations is the application of phospholipid vesicles to improve the solubility of such hydrophobic drugs. Ethosomes are one of the most powerful phospholipid vesicles used to achieve that that target. In this study, LUT-loaded ethosomal nanoparticles (LUT-ENPs) were prepared by the cold method. Full factorial design and response surface methodology were used to analyze and optimize the selected formulation variables. Drug entrapment efficiency, vesicle size, zeta potential, Fourier transform infra-red spectroscopy, scanning electron microscopy, and cumulative percent drug released was estimated. The selected LUT-ENPs were subjected to further investigations as estimation of hepatic gene expression levels of GPC3, liver biomarkers, and oxidative stress biomarkers. The prepared LUT-ENPs were semi-spherical in shape with high entrapment efficiency. The prepared LUT-ENPs have a small particle size with high zeta potential values. The in vitro liver biomarkers assay revealed a significant decrease in the hepatic tissue nitric oxide (NO), malondialdehyde (MDA) content, and the expression of the GPC3 gene. Results showed a high increase in the hepatic tissue levels of glutathione (GSH) and superoxide dismutase (SOD). Histopathological examination showed a small number of hepatic adenomas and a significant decrease of neoplastic hepatic lesions after treatment with LUT-ENPs. Our results firmly suggest the distinctive anti-proliferative activity of LUT-ENPs as an oral drug delivery system for the treatment of HCC.

show abstract

Tailoring and Optimization of Nifedipine Controlled Release Organogel via Quality by Design Approach

Dave,

Kariya,

Dudhat

2024

J Pharm Innov

View full text Add to dashboard Cite

Response Surface Methodology as a Useful Tool for Development and Optimization of Sustained Release Ketorolac Tromethamine Niosomal Organogels

Cited by 13 publications

References 43 publications

Novel Green Biosynthesis of 5-Fluorouracil Chromium Nanoparticles Using Harpullia pendula Extract for Treatment of Colorectal Cancer

Novel Green Biosynthesis of 5-Fluorouracil Chromium Nanoparticles Using Harpullia pendula Extract for Treatment of Colorectal Cancer

Design and Optimization of Orally Administered Luteolin Nanoethosomes to Enhance Its Anti-Tumor Activity against Hepatocellular Carcinoma

Tailoring and Optimization of Nifedipine Controlled Release Organogel via Quality by Design Approach

Contact Info

Product

Resources

About