Responses of the CYP1A biomarker in Jenynsia multidentata and Phalloceros caudimaculatus and evaluation of a CYP1A refractory phenotype

Chivittz, Cíntia C.; Pinto, Debora P.; Ferreira, Roger Stacke; Sopezki, Maurício da Silva; Fillmann, Gilberto; Zanette, Juliano

doi:10.1016/j.chemosphere.2015.08.050

Cited by 18 publications

(8 citation statements)

References 25 publications

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“…The sampling site in the reference stream (32 33 0 32.48 00 S, 52 23 0 54.59 00 W) is a watercourse located 14 km away from the closest inhabited urban area at Cassino Beach, so it is relatively less impacted by anthropogenic contamination (Figure 1). Chivittz et al (2016) analyzed the sum of 16 EPA priority PAHs in sediment in those sites, confirming very different levels of PAHs between the polluted and reference streams (4414.0 and 1.7 ng g À1 dry weight, respectively).…”

Section: Sampling Selection and Site Characterizationmentioning

confidence: 82%

“…Similar to other cyprinodontiform fishes, e.g., Poecilia reticulata and Fundulus heteroclitus, it presents characteristics that favor its use as a model in environmental toxicology studies (Zanette, 2013). The P. caudimaculatus fish has been suggested as a model to evaluate the effects of pollution in the aquatic environment (Araújo et al, 2009;Chivittz et al, 2016;Ferreira et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Environmental contamination alters the intestinal microbial community of the livebearer killifish Phalloceros caudimaculatus

Nolorbe-Payahua

Freitas

Roesch

et al. 2020

Heliyon

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Intestinal microbiota perform important functions for the health of fishes. Knowing the microbial composition and evaluating the possible effects caused by anthropogenic pollution in the intestinal microbiota of fish populations might represent an important step in defining microbial biomarkers for water pollution. This study evaluated the impact of environmental contamination on the gut microbiota of the livebearer killifish Phalloceros caudimaculatus. The 16S survey using the V4 region of the 16S rRNA gene was used to characterize and compare the microbiota of two P. caudimaculatus populations from streams with different levels of environmental contamination in Rio Grande, RS, Brazil. Twelve bacterial operational taxonomic units (OTUs) (around one-third of the total) were shared between both fish populations. They represent the core microbiota of the gut in this species. The dominant phyla were Protebacteria and Firmicutes, with more than 80% of relative abundance. The dominant genus was Burkholderia with more than 35% of the relative abundance irrespective of the environmental condition. We detected a lower microbial diversity (Shannon index and observed OTUs) in fish from the polluted stream compared to the reference stream. The PERMANOVA analysis showed that the intestinal microbial communities from fish living in the polluted stream were distinct from those found in the reference stream (p < 0.05). Finally, we identified Luteolibacter, Methylocaldum and Rhodobacter genera, which correlated strongly with the polluted stream. These taxa might represent potential microbial biomarkers of exposure to environmental contaminants in the guts of fish. Confirmation of these findings in other polluted environments might allow the development of a microbiota-based screening approach for environmental evaluation in ecotoxicological studies in aquatic ecosystems.

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Section: Sampling Selection and Site Characterizationmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Environmental contamination alters the intestinal microbial community of the livebearer killifish Phalloceros caudimaculatus

Nolorbe-Payahua

Freitas

Roesch

et al. 2020

Heliyon

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“…The liver is the primary organ in vertebrates, and it is involved in a wide range of functions like detoxification, protein synthesis, digestion and excretion. It regulates the expression of enzymes, which are involved in the biotransformation of xenobiotics (Chivittz et al., 2016; Ivana Haluzová et al., 2011). A large portion of the xenobiotics introduced into animals is accumulated by the liver, which can damage it.…”

Section: Discussionmentioning

confidence: 99%

Structural damage in liver, gonads, and reduction in spawning performance and alteration in the haematological parameter of Anabas testudineus by glyphosate‐ a herbicide

et al. 2020

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The increasing demand for herbicides in agricultural fields affected aquatic environments as they entered into water bodies by agricultural runoff. Glyphosate (GLY), an organophosphate herbicide, is widely used in India for weed control. This study aimed to evaluate the toxic effects of GLY on climbing perch Anabas testudineus by studying its effects on the gonadosomatic index (GSI), hepatosomatic index (HSI), gonad and liver histology, spawning performance, and haematological parameters (RBC, WBC, haemoglobin). In this study, Anabas testudineus were exposed to three sub‐lethal concentrations of GLY such as T1: 2.6 ppm (1/30th of 48h LC50), T2: 3.9 ppm (1/20th of 48h LC50) and T3: 7.8 ppm (1/10th of 48 hr LC50) for 45 days with 12 fish in each tank. Results indicated a significant decrease in GSI, HSI, structural alterations in testis, and ovary on 30th and 45th days of exposure. Vacuolation in tissue, dilution of sinusoid, altered tissue architecture in the liver, reduction in spawning performances (fertilization and hatching rate), increase in WBC count, decrease in RBC number and haemoglobin content were observed in all treatment groups on the 45th day exposure. Thus, the outcome of the present study revealed that the herbicide could deleteriously impair reproductive fitness, alter the liver structure, and haematological parameter of A. testudineus with a possible reduction in growth and population size.

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“…CYP1A expression has been used in the past as a bioindicator of pollution. However, some fish may adapt to pollution and contamination over time so that they eventually express much lower levels of CYP1A protein (Chivittz et al, 2016, Wills et al, 2009. This study is the first of its kind in western New York and should be continued so that CYP1A expression can be used to monitor pollution levels in various bodies of water.…”

Section: Discussionmentioning

confidence: 99%

CYP1A expression in freshwater fish of western New York as an indicator of pollution levels

Williams¹,

Koetsier²,

Johnson³

et al. 2021

Preprint

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Various species of freshwater fish regulate the expression of certain proteins in response to environmental contamination. Previous research has shown that CYP1A expression increases in response to contaminant levels, and can result in increased tumor formation. Fish in contaminated environments would thus benefit by downregulating the expression of CYP1A to reduce tumor prevalence as an adaptive strategy. Alternatively, regulation of the CYP1A protein in fish can serve as a bioindicator of the pollution level of an environment. This study evaluated CYP1A expression in twelve different species of freshwater fish from seven bodies of water throughout western NY including Cuba Lake, Genesee River, Hanging Bog, Love Canal, Moss Lake, Rushford Lake and Tifft Nature Preserve. Western blot analysis was used to measure CYP1A expression as a marker of site pollution and potential fish population adaptation. It was hypothesized that low CYP1A expression at a site with known contamination would suggest signs of adaptation to pollution levels present. Furthermore, if at least one sample from a species showed CYP1A expression, then the CYP1A antibody (Caymen Chemical, USA; 173132) had compatibility with that species, eliminating falsely suspected adaptation. The results from this study suggest possible adaptation of fish may be occurring in the polluted Tifft Nature Preserve and Genesee River. In contrast, CYP1A expression in fish from Cuba Lake, Hanging Bog, Love Canal, Moss Lake, and Rushford Lake appear to represent known pollution levels and adaptation is not likely occurring. Results from this study are preliminary and next steps include collection and analysis of sediment to provide a stronger correlation between pollution at sites and CYP1A expression.

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Responses of the CYP1A biomarker in Jenynsia multidentata and Phalloceros caudimaculatus and evaluation of a CYP1A refractory phenotype

Cited by 18 publications

References 25 publications

Environmental contamination alters the intestinal microbial community of the livebearer killifish Phalloceros caudimaculatus

Environmental contamination alters the intestinal microbial community of the livebearer killifish Phalloceros caudimaculatus

Structural damage in liver, gonads, and reduction in spawning performance and alteration in the haematological parameter of Anabas testudineus by glyphosate‐ a herbicide

CYP1A expression in freshwater fish of western New York as an indicator of pollution levels

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