Restoration of rooting competence in a mature plant of Garcinia indica through serial shoot tip grafting in vitro

Chabukswar, Meera M; Deodhar, Manjushri A.

doi:10.1016/j.scienta.2005.12.008

Cited by 18 publications

(11 citation statements)

References 14 publications

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“…Similar responses of root induction by IBA and NAA were reported in Garcinia indica (Malik et al 2005, Thengane et al 2006, Deodhar et al 2008. In contrast to the above, half-strength WPM with 2.5 -245 µM IBA did not induce any rooting in Garcinia indica (Chabukswar et al 2006). It was reported that IBA promotes better root induction in plants such as Azadirachta indica (Srivastava et al 2009), Acanthophyllum sordidum (Meratan et al 2009), Bixa orellana (Parimalan et al 2009) and Plantago algarbiensis (Goncalves et al 2009).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 77%

High frequency plant regeneration from the mature seeds of Garcinia indica

Baskaran¹,

Krishnan²

2011

Biologia plant.

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A high frequency plant regeneration system was developed for the production of high yielding elite clones of Garcinia indica via direct organogenesis. A maximum number of 86.2 shoot buds per explant were induced from the mature seed segments cultured on Woody plant medium (WPM) supplemented with 2.0 mg dm -3 N 6 -benzyladenine and 1 mg dm -3 indole-3-acetic acid. Rooting was achieved on half-strength WPM medium supplemented with 3 mg dm -3 indole-3-butyric acid. Rooted plantlets were acclimatized and transferred to greenhouse for further growth. The highest survival rate of 95 % was recorded using a mixture of garden soil and sand. Histological studies clearly revealed multiple shoot formation from mature seed segments.

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Section: ⎯⎯⎯⎯mentioning

confidence: 77%

High frequency plant regeneration from the mature seeds of Garcinia indica

Baskaran¹,

Krishnan²

2011

Biologia plant.

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“…When nodal explants from mature trees were used as explants, WPM medium with 8.9 μM BAP gave 2.6 shoots per explant. When 8.9 μM BAP was supplemented with 0.5 μM TDZ the shoot number increased to five per explant (Chabukswar and Deodhar 2006;loc. cit.).…”

Section: Discussionmentioning

confidence: 95%

“…For cloning elite genotypes, explants from 10-to 12-yr-old mature trees were used, but axillary shoots obtained from nodal explants of G. indica did not induce rooting. Hence, efforts were made to standardize a protocol for serial shoot tip grafting in which in vitro shoots obtained from a nodal explant of a mature tree were used as scions and were grafted repeatedly onto in vitro-grown seedlings to achieve the desired level of rooting (Chabukswar and Deodhar 2006).…”

Section: Introductionmentioning

confidence: 99%

Clonal propagation and hydroxycitric acid production from in vitro shoot cultures of Garcinia indica using root suckers as explant

Tembe¹,

Deodhar²

2011

In Vitro Cell.Dev.Biol.-Plant

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In vitro shoots from mature tree explants often show poor root initiation response. Garcinia species are known for vegetative propagation through root suckers in natural environment. To establish a protocol for clonal propagation, apical and intercalary buds of the root suckers obtained from a mature elite tree of Garcinia indica were used as explant source. Multiple shoots were initiated in woody plant medium fortified with combinations of 6-benzylaminopurine and 1-phenyl-3-(1,2,3-Thiadiazol-5-YL)-urea. A pulse treatment of indole-3-butyric acid ranging from 4.9 to 19.6 mM for 30 s and 1 min was tried for root induction. The resulting in vitro plants were successfully hardened. The initiated shoots were also used for in vitro synthesis of hydroxycitric acid in shake flask cultures. The effect of basal medium, incubation period and plant growth regulators on production of hydroxycitric acid in vitro was studied.

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“…Khalafalla ve Daffalla (2008) tarafından senegal akasya'sında 1.5-3.0 cm uzunluğundaki aşı kalemleri kullanılarak %70-90 arasında değişen mikroaşı başarısı elde ettiğini bildirmiştir. Kiraz ağacı ve 20 yaşındaki Garcinia indica (Kokum) ağaçlarında sırasıyla 0.4-1 mm ve 0.5-1 cm uzunluğunda sürgün kullanımının mikroaşılama için uygun olacağı bildirilmektedir (Deogratis et al, 1986;Chabukswar and Deodhar, 2006). Hem gençleştirme hem de bitki sağlığı amacıyla aşı kalemi büyüklüğünün uygun şekilde düşürülmesi gerekmektedir.…”

Section: Aşı Kalemi a Tür -çEşit Etkisiunclassified

“…Bunun nedeninin ise aktif dönemde bulunan bitki bünyesindeki oksin metabolizması ve peroksidaz aktivitesinden kaynaklandığı öne sürülmüştür (Poessel et al, 1980;Jonard, 1986). Elma, kiraz, hindistan eriği, şemsiye akasya, antepfıstığı, kokum gibi belli bazı türlerde yapılan çalışmalarda in vitro mikroçelik kullanımının, in vivo çelik kullanımından daha iyi olduğu bildirilmiştir (Detrez, 1994;Chabukswar and Deodhar, 2006). Mikroaşıda elde edilen başarının, aslında bitkinin gelişme durumuna göre bir dalgalanma gösterdiği yapılan bazı çalışmalarda bildirilmektedir.…”

Section: Fizyolojik Koşullarunclassified

In vitro Micrografting of Woody Plant Species-I (Rootstock, Scion, Grafting Technique)

Çalar¹,

Onay²,

Karlıdağ³

et al. 2017

jist

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ÖZET: İn-vitro aşılama, aksenik kültür koşullarında minyatür aşı kalemlerinin aşılanmasını kapsayan ve diğer tekniklere nazaran en son vejetatif çoğaltım tekniklerinden biridir. Bu yöntem, sürgün ucu kültürü ve aşılamanın bazı sınırlayıcı özelliklerinin üstesinden gelmekle birlikte, her iki metodun avantajlarını da bir arada bulundurmaktadır. İlk uygulandığı zamanlarda bazı meyve tür ve çeşitlerindeki virüs ve benzeri endojen patojenlerin eradikasyonu için geliştirilen in-vitro aşılama, bitki gelişim ve fizyolojisinin farklı alanlarında çeşitli odunsu bitki türlerinde hızla gelişmiştir. Bunlar birçok odunsu türlerin olgun genotiplerinin klonal çoğaltımında bir ön koşul olarak fizyolojik rejuvenasyonu ve aşıda uyuşmazlığı da kapsamaktadır. Sonuç olarak in-vitro aşılama, yoğun olarak kullanılan ve diğer vejatatif çoğaltım metodlarında bulunan olumsuzlukların üstesinden gelebilmek için daha çok düşünülmeyi ve kullanılmayı hak eden orijinal ve tecrübe gerektiren bir tekniktir. Doku ve hücreler arasındaki genetik benzerlik ve farklılıkların ayrıntılı incelenmesine imkan sağlamaktadır. Bu çalışmanın amacı, 1970'li yıllarda turunçgillerde virüsten ari bitki üretimi amacıyla başlayan in vitro aşılama çalışmalarının günümüze kadar nasıl bir süreçten geçtiği ve özellikle meyve türlerinin de dahil olduğu odunsu bitki türlerinde bitki ıslahı ve çoğaltımı amacıyla ne tür çalışmaların yapıldığının ortaya çıkarılması ve belli bir düzende sunulmasıdır.Anahtar Kelimeler: Aşılama, in-vitro, kalem ABSTRACT: In vitro micrografting is a propagation technique, involving the grafting of relatively miniature cuttings under axenic culture conditions and it is one of the recent developed propagation techniques compared to other conventional vegetative propagation techniques. This method overcomes some of the limitations of shoot tip culture and grafting, while it also keeps together the advantages of both methods. Micrografting was applied for the eradication viruses and pathogens from some fruit species and cultivars during the first application period, but later, the technique was further developed on various woody plant species in different research areas of plant physiology and development. These includes physiological rejuvenation and incompatibility grafting as a prerequisite for the clonally propagation of mature genotypes of many woody species. Consequently, in vitro micrografting is used in large scale propagation and an original technique which needs experience by overcoming the disadvantages of other propagation technique. It also enables to examine in detail the genetic similarities and differences between the tissues and cells. The aims of this study were (1) to review how micrografting studies passed a process from 1970s until today, first started to obtain virus-free plants from citrus; (2) to reveal what kind of work has been presented particularly on the plant breeding and propagation of the woody plant species, including the type of fruit breeding and reproduction and (3) and to present those studies in a specific order.

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Restoration of rooting competence in a mature plant of Garcinia indica through serial shoot tip grafting in vitro

Cited by 18 publications

References 14 publications

High frequency plant regeneration from the mature seeds of Garcinia indica

High frequency plant regeneration from the mature seeds of Garcinia indica

Clonal propagation and hydroxycitric acid production from in vitro shoot cultures of Garcinia indica using root suckers as explant

In vitro Micrografting of Woody Plant Species-I (Rootstock, Scion, Grafting Technique)

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