Restriction Endonuclease Staining Profiles in the C-Heterochromatin of Cervidae

Gripenberg, Ulla; Wessman, Maija

doi:10.1111/j.1601-5223.1993.00251.x

Hereditas

2004

DOI: 10.1111/j.1601-5223.1993.00251.x

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Restriction Endonuclease Staining Profiles in the C-Heterochromatin of Cervidae

Ulla Gripenberg

Maija Wessman

Abstract: The four species of Cervidae (Rangifer tarandus, Odocoileus uirginianus, Akes alces, and Ceruus dama) show striking differences in size, morphology, banding pattern, and amount of heterochromatin in their sex chromosomes studied here. Cervus dama is characterized by an acrocentric X chromosome, representing the original X in Cervidae. Odocoileus uirginianus and Alces alces have submetacentric and otherwise structurally different X chromosomes. In Rangifer tarandus the X and the Y are spectacularly large sex ch… Show more

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Cited by 3 publications

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“…The RE-induced banding of the extraordinary heterochromatin in the sex chromosomes is reported separately in part I1 of this publication ( GRIPENBERG and WESSMAN 1993). The evolution of the autosomes on one hand and the sex chromosomes on the other hand has proceeded along different and presumably independent pathways.…”

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confidence: 86%

Restriction Endonuclease Staining Profiles in the C-heterochromatin of Cervidae

Wessman

Gripenberg²

2004

Hereditas

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The amount of C‐heterochromatin in the autosomes of Rangifer tarandus, Odocoileus virginianus, Alces alces, and Cervus dama was estimated as small, medium, or large. The richness of certain specific base sequences in the DNA of the highly repetitive heterochromatin was studied by selective staining methods and by in situ treatment of chromosome spreads with restriction endonucleases. Resistant and susceptible regions, distributed in specific domains of the heterochromatin, expressed similar staining profiles in all three species belonging to the same evolutionary lineage, while the species belonging to another evolutionary lineage showed a clearly different organization. Correlations are demonstrated between the patterns of the specific base sequences here studied and structural chromosome changes characterizing the evolutionary lineages in Cervidae.

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mentioning

confidence: 86%

Restriction Endonuclease Staining Profiles in the C-heterochromatin of Cervidae

Wessman

Gripenberg²

2004

Hereditas

Self Cite

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Comparative FISH mapping of bovine cosmids to reindeer chromosomes demonstrates conservation of the X-chromosome

et al. 1996

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Three X chromosome-specific bovine cosmids were used for fluorescence in situ hybridization mapping on reindeer (Rangifer tarandus) chromosomes, to test whether such large genomic clones could be used for comparative mapping across distantly related species. All three cosmids showed distinct unique hybridization sites on the reindeer X. Comparative map locations of these cosmids, together with the relative C-banding and genome size data on the X chromosomes of the two species, provide preliminary indications that the short and long arms of bovine X correspond, respectively, to the long and short arms of the reindeer X. The study also demonstrates that cosmid clones can be used successfully for comparative mapping across species that diverged 35 million years ago.

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Defining the anatomy of the Rangifer tarandus sex chromosomes

et al. 1998

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A comprehensive cytogenetic characterization of the unusally large reindeer (Rangifer tarandus) sex chromosomes is presented for the purpose of studying the evolution of these atypical gonosomes. Sex chromosome idiograms were constructed from G-banded and C-banded chromosomes to illustrate the relative amounts and locations of euchromatin and heterochromatin. Hybridization with a Mazama gouazoubira X whole-chromosome paint revealed that essentially all reindeer X-linked euchromatin and most reindeer Y-linked euchromatin is conserved interspecifically. Subsequently, painting probes were generated from flow-sorted reindeer X chromosomes, flow-sorted reindeer Y chromosomes, and from microdissections of specific gonosomal regions to establish specific segment-to-segment homologies between these gonosomes. In particular, one microdissection-generated paint demonstrated that certain constituent repetitive DNAs, found in C-band region Xq31, were also present in essentially all heterochromatin blocks of the Y chromosome. Microdissection-generated paints from other X-linked heterochromatin blocks revealed the presence of DNA sequences that lacked homologous sequences on the Y chromosomes and were more specific for their region of origin. These characteristics of the reindeer sex chromosomes are consistent with the notion that mammalian sex chromosomes were derived from homologous progenitor chromosome pairs and provide insights into the evolution of these atypical mammalian gonosomes.

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Restriction Endonuclease Staining Profiles in the C-Heterochromatin of Cervidae

Cited by 3 publications

References 15 publications

Restriction Endonuclease Staining Profiles in the C-heterochromatin of Cervidae

Restriction Endonuclease Staining Profiles in the C-heterochromatin of Cervidae

Comparative FISH mapping of bovine cosmids to reindeer chromosomes demonstrates conservation of the X-chromosome

Defining the anatomy of the Rangifer tarandus sex chromosomes

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