Restriction fragment length polymorphisms of medicinal plants and crude drugs. IV. Restriction Fragment Length Polymorphisms of rDNA and Variation of Essential Oil Composition in Atractylodes Plants.

Mizukami, Hajime; Shimizu, Ryosuke; Kohda, Hiroshi; Kohjyouma, Mareshige; Kawanishi, Fumiaki; Hiraoka, Noboru

doi:10.1248/bpb.19.577

Cited by 14 publications

(7 citation statements)

References 3 publications

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“…The average content decreased to 1.92 mg/g dry weight in the processed Atractylodis Rhizoma because atractylon was readily oxidized to atractylenolides II and III during processing. The atractylon contents in the dried rhizomes of A. ovata and A. japonica were also evaluated by gas-liquid chromatography (GLC) [15]. The average atractylon content was 13.0 mg/g dry weight in A. ovata rhizomes and 22.0 mg/g dry weight in A. japonica rhizomes.…”

Section: Nmr Analysis and Purity Of Atractylonmentioning

confidence: 99%

Quantitative determination of atractylon in Atractylodis Rhizoma and Atractylodis Lanceae Rhizoma by 1H-NMR spectroscopy

et al. 2010

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(1)H-NMR spectroscopy was successfully applied to the quantitative determination of atractylon in Atractylodis Rhizoma (dried rhizomes of Atractylodes ovata and A. japonica) and Atractylodis Lanceae Rhizoma (dried rhizomes of Atractylodes lancea and A. chinensis). The analysis was carried out by comparing the integral of the H-12 singlet signal of atractylon, which was well separated in the range of delta 6.95-7.05 ppm in the NMR spectrum, with the integral of a hexamethyldisilane (HMD) signal at delta 0 ppm. The atractylon contents obtained by the (1)H-NMR spectroscopy were consistent with those obtained by the conventional HPLC analysis. The present method requires neither reference compounds for calibration curves nor sample pre-purification. It also allows simultaneous determination of multiple constituents in a crude extract. Thus, it is applicable to chemical evaluation of crude drugs as a powerful alternative to various chromatographic methods.

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Section: Nmr Analysis and Purity Of Atractylonmentioning

confidence: 99%

Quantitative determination of atractylon in Atractylodis Rhizoma and Atractylodis Lanceae Rhizoma by 1H-NMR spectroscopy

et al. 2010

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“…Many Chinese medicinal prescriptions contain it as the principle drug. The essential oil has been isolated and is the major constituent of Atractylodes Rhizoma; it has several pharmacological functions [1], [2], [3], [4], [5]. The extract of the herb has anti-inflammatory [6] and anti-ulcer properties [7], [8], [9], scavenges the CCl 3 radical, inhibits lipid peroxidation [10], [11] and xanthine oxidase inhibition [12], [13], inhibits the tert-butyl hydroperoxide-induced cytotoxicity and lipid peroxidation in primary culture of rat hepatocytes [14], [15], and inhibits the growth of esophageal carcinoma cells.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Activity of Sesquiterpenoids from Atractylodes ovata on Leukemia Cell Lines

Wang¹,

Chen²,

Yang³

2002

Planta med

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The rhizome of Atractylodes ovata (Bai Zhu in Chinese) is a widely used traditional Chinese herb in Taiwan as a tonic agent. In this paper, four sesquiterpenoids, namely atractylon, and atractylenolides I, II, and III, were isolated from the n-hexane extract of A. ovata and were evaluated for cytotoxic effects in vitro. Atractylon significantly inhibited the growth of human leukemia cell line HL-60 and mouse leukemia cell line P-388, and showed low cytotoxicity against primary cultures of normal human peripheral blood mononuclear cells at 15 microg/ml for 12 h. Atractylon had a dose-dependent antiproliferative effect on the two tumor cell lines. In accordance with DNA fragment increases and PARP protein decreases, atractylon at 15 microg/ml for 6 h induced apoptosis in HL-60 cells. Moreover, atractylon inhibited the viability of P-388 cells and induced apoptosis after 15 microg/ml treatment for 12 h in an in vitro assay. However, atractylenolide I at 30 microg/ml for 12 h also induced apoptosis in HL-60 and P-388 cells, but atractylenolides II and III showed no significant inhibition effects on tumor cell growth. As the above results suggested, atractylon and atractylenolide I were the major cytotoxic principle constituents of A. ovata on leukemia cell lines.

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“…The analysis of sequence variation in the regions such as ribosomal DNA (rDNA) and a large subunit of ribulose-1,5-bisphosphate carboxylase (rbcl) has become an effective method for identification of medicinal herbs. The PCR-RFLP of rDNA or rbc1 has been successfully carried out on Glehnia (Mizukami et al, 1993), Epimedium (Nakai et al, 1996), Atractylodes (Mizukami et al, 1996;, Panax (Ngan et al, 1999), Codonopsis (Fu et al, 1999), Dendrobium (Zhang et al, 2005), Fritillaria (Wang et al, 2005;, Sinopodophyllum and Dysosma (Gong et al, 2006), Ganoderma (Zhou et al, 2008), Akebia (Kitaoka et al, 2009) and Lonicera (Peng et al, 2010).…”

Section: Polymerase Chain Reaction-restriction Fragment Length Polymomentioning

confidence: 99%

DNA markers in the authentication of Traditional Chinese Medicine

Liu¹,

Tan²,

Qi³

et al. 2014

J. Med. Plants Res.

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Traditional Chinese medicine (TCM), with its multi-target effects, is gaining more and more attention all over the world due to its specific theory and long historical clinical practice. But the absence of an objective and accurate inspection system is frequently cited as one of the major hurdles for its modernization and globalization. Benefiting from modern molecular biology and polymerase chain reaction (PCR) techniques, DNA markers have become one of the most reliable methods for identification and authentication of Chinese medicinal materials. This paper reviewed the recent progress of DNA markers, including PCR-based markers, hybridization-based markers, sequencedbased markers, and DNA microarray in the authentication of TCM.

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Restriction fragment length polymorphisms of medicinal plants and crude drugs. IV. Restriction Fragment Length Polymorphisms of rDNA and Variation of Essential Oil Composition in Atractylodes Plants.

Cited by 14 publications

References 3 publications

Quantitative determination of atractylon in Atractylodis Rhizoma and Atractylodis Lanceae Rhizoma by 1H-NMR spectroscopy

Quantitative determination of atractylon in Atractylodis Rhizoma and Atractylodis Lanceae Rhizoma by 1H-NMR spectroscopy

Cytotoxic Activity of Sesquiterpenoids from Atractylodes ovata on Leukemia Cell Lines

DNA markers in the authentication of Traditional Chinese Medicine

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