Restriction site mapping of four genome types of adenovirus types 3 and 7 isolated in South America

Niel, Christian; Moraes, Marcia Terezinha Baroni de; Mistchenko, Alicia; Leite, José Paulo Gagliardi; Gomes, Saint Clair

doi:10.1002/jmv.1890330211

Cited by 25 publications

(33 citation statements)

References 30 publications

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“…This same genotype was found to become prevalent in Chile during 1986 (Kayon & Suarez 1990). Later, Niel et al (1991) ognized (Kayon & Wadell 1992) as the same virus and belonging to serotype 7, genetic type 7h.…”

mentioning

confidence: 78%

“…Strains showing these features had been previously classified as intermediates (Matumoto et al 1958, Fukumi et al 1961, Rafjko 1967. Using viral DNA digestion with restriction enzymes and comparing with Ad3 and Ad7 maps described earlier, it was possible to classify these isolates as Ad3 variant Ad3h, recently described in one isolate from Argentina (Niel et al 1991).…”

Section: Discussionmentioning

confidence: 99%

“…This is the first report of Ad3h isolates in Brazil. Ad3 genomic types previously described in Brazil were Ad3p1 (Li & Wadell 1988), Ad3e1 (Gomes et al 1989) and Ad3e2 (Niel et al 1991). These isolates were obtained from faecal specimens.…”

Section: Discussionmentioning

confidence: 99%

“…Ad7e was the original strain isolated in Brazil (Wadell et al 1985) and Ad3h and Ad7h were the original strains isolated in Argentina (Niel et al 1991). Ad3 and Ad7 rabbitt antisera were obtained from VRD/CPHL and also from MRC, Mill Hill, UK.…”

Section: Methodsmentioning

confidence: 99%

“…Restriction maps of Ad3p and Ad3h DNA for the endonucleases BamHI, BclI, EcoRI, HindlII, KpnI, SalI and SmaI were obtained from Niel et al (1991 Adrian et al (1989), Li and Wadell (1986), Niel et al (1991) and Wadell et al (1981).…”

Section: Methodsmentioning

confidence: 99%

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Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test

Moraes

Silva

Leite

et al. 1998

Mem. Inst. Oswaldo Cruz

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mentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test

Moraes

Silva

Leite

et al. 1998

Mem. Inst. Oswaldo Cruz

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Molecular epidemiology of adenovirus acute lower respiratory infections of children in the South Cone of South America (1991–1994)

et al. 1996

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A collection of 165 adenovirus strains isolated from nasopharyngeal aspirates of children hospitalized for acute lower respiratory infection in Argentina, Chile, and Uruguay between 1991 and 1994 was studied by restriction enzyme analysis (work performed in the Department of Virology, University of Umeå). Of the isolates, 71% (n = 117) were identified as members of subgenus B. Of these, 101 (61.2%) corresponded to genome type 7h, four (2.4%) to genome type 3p2, four (2.4%) to genome type 11a, one (0.6%) to genome type 7b, and one (0.6%) to genome type 7c. Two isolates that were neutralized as serotype 3 and four isolates that were neutralized as serotype 7 exhibited novel BamHI cleavage profiles corresponding to three new genome types denominated 3x, 7i, and 7j. Subgenus C members represented 28.5% of all typed isolates. Five different genome types of Ad1, seven genome types of Ad2, and three genome types of Ad5 were identified of, which two, two, and one, respectively, were found to correspond to new DNA variants. Only one isolate (0.6%) corresponded to Ad4 of subgenus E. Ad7h was isolated from 17 of the 18 fatal cases recorded among the patients included in the study.

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Fatal adenovirus infection associated with new genome type

et al. 1998

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The first fatal case caused by the new genome type 7i is described in an 8-month-old boy requiring long-term respiratory support who developed Reye's syndrome, acute respiratory distress, and bronchiolitis obliterans with fatal evolution. Adenovirus was detected in nasopharyngeal secretions and was persistently positive during hospitalization. IgM and IgG adenovirus antibody titers measured in serum by enzyme-linked immunoassay (EIA) were 1:32 and 1:800, respectively. Serum interleukins (IL) and interferons (IFN) measured by EIA were as follows: IL-2, 110 pg/ml; IL-6, 300 pg/ml; IL-8, 7,000 pg/ml; TNF-alpha, 35 pg/ml, IL-1 and IL-4 undetectable, IFN-alpha 2,200 pg/ml, and IFN-gamma 700 pg/ml. Virologic studies showed that adenovirus isolated belonged to subgenus B, and digestion of viral DNA with Bam HI, Sma I, Bgl II, and Hind III identified the isolate as belonging to genome type 7i. Autopsy showed bronchiolitis obliterans with diffuse alveolar damage and perivenular fatty degeneration with polymorphonuclear infiltrates in the periportal spaces. The difficulty in obtaining adequate oxygenation with minimization of iatrogenic oxygen injury is discussed.

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Restriction site mapping of four genome types of adenovirus types 3 and 7 isolated in South America

Cited by 25 publications

References 30 publications

Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test

Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test

Molecular epidemiology of adenovirus acute lower respiratory infections of children in the South Cone of South America (1991–1994)

Fatal adenovirus infection associated with new genome type

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