Resveratrol ameliorates methotrexate-induced hepatotoxicity in rats via inhibition of lipid peroxidation

Dalaklıoğlu, Selvinaz; Genc, Gizem Esra; Aksoy, Nurkan; Akçit, Ferhat; Gümüşlü, Saadet

doi:10.1177/0960327112468178

Cited by 107 publications

(76 citation statements)

References 57 publications

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“…There is an increasing interest in RV, since RV may prevent or delay the ischemic and chemicallyinduced injuries, multi-organ dysfunctions, inflammations, infections, and heart disorders by inhibiting oxidation and the expression of inflammatory mediators and also by regulating microcirculation (3-6,9,27). Dalaklioglu et al (28) found that RV treatment (20 mg/kg/day, ip) significantly prevented methotrexate-induced hepatotoxicity, as indicated by alanine transaminase, aspartate aminotransferase, and alkaline phosphatase levels and liver histopathology. RV significantly reduced the elevated levels of TBARS and activities of catalase and glutathione-S transferase in the hepatic tissue compared to the methotrexate-treated group.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol Protects Sepsis-Induced Oxidative DNA Damage in Liver and Kidney of Rats

Aydın¹,

Şahin²,

Bacanlı³

et al. 2016

Balkan Med J

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Background:The increases of free radicals have been proposed to be involved in the pathogenesis of sepsis, which leads to multiple-organ dysfunction syndromes. The uses of antioxidants as a complementary tool in the medical care of oxidative stress-related diseases have attracted attention of researchers. Resveratrol (RV) has suggested being antioxidant, anti-proliferative, and anti-inflammatory effects in various experimental models and clinical settings. Aims: This study was undertaken to evaluate the protective effects of RV on oxidative DNA damage induced by sepsis in the liver and kidney tissues of Wistar albino rats. Study Design: Animal experimentation. Methods: Four experimental groups consisting of eight animals for each was created using a total of thirty-two male Wistar albino rats. Sham group was given 0.5 mL of saline intra-peritoneal (ip) only following laparatomy. Sepsis group was given 0.5 mL saline ip only following the induction of sepsis. RV-treated group was given a dose of 100 mg/kg ip RV in 0.5 mL saline following laparatomy. RVtreated sepsis group was given 100 mg/kg ip RV in 0.5 mL saline following the induction of sepsis. A model of sepsis was created by cecal ligation and puncture technique. In the liver and kidney tissues, oxidative stress parameters (malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPX)) and a proinflammatory cytokine (tumor necrosis factor alpha (TNF-alpha)), were evaluated spectrophotometrically and DNA damage was determined by the alkaline single cell gel electrophoresis (comet assay) technique using formamidopyrimidine DNA glycosylase protein. Results:In the RV-treated sepsis group, the levels of MDA and TNF-alpha were lower and GSH levels, SOD and GPX activities were higher than in the septic rats (p<0.05). RV treatment significantly reduced the sepsis-induced oxidative DNA damage in the liver and kidney cells (p<0.05). Conclusion: It is suggested that RV treatment might reduce the sepsis-induced oxidative DNA damages in sepsis-related diseases; however, there is a need for more studies to clear up the protective mechanisms of RV against sepsis.

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Section: Discussionmentioning

confidence: 99%

Resveratrol Protects Sepsis-Induced Oxidative DNA Damage in Liver and Kidney of Rats

Aydın¹,

Şahin²,

Bacanlı³

et al. 2016

Balkan Med J

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“…Both techniques proved that the reticuloendothelial cells were damaged by ionizing radiation, which might result in malfunction of the Kupffer cells and or a decrease in their number [28]. Histological findings of MTX-induced hepatotoxicity are including macrovesicular steatosis, hepatocellular necrosis and inflammation, edema, fibrosis, morphological abnormalities and development of cirrhosis [29][30][31]. Chemotherapeutic agents caused drug induced acute hepatitis and hepatomegaly, decreased liver enhancement and widening of periportal space due to edema which are nonspecific imaging findings of acute hepatitis [32].…”

Section: Discussionmentioning

confidence: 99%

The use of 99mTc-phytate for assessment the protective effect of vitamin E against hepatotoxicity induced by methotrexat in rat

et al. 2018

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Hepatotoxicity is one of the most common side effects of methotrexate (MTX) therapy in patients. The aim of this study was to evaluate the protective effect of vitamin E against MTX-induced hepatotoxicity using 99m Tc-phytate as a radiopharmaceutical agent in animals. Rats were divided into five groups as follows: control, solvent, Vit E (100 mg/kg), MTX (20 mg/kg) and Vit E + MTX. Animals were intraperitoneally injected with Vit E for 17 days before MTX injection and continued for 4 days. Tc-phytate was injected into the tail of rats after the 21 days of Vit E administration. Percentage of the injected dose per gram of liver and spleen tissues (%ID/g) was calculated in treated rats. Liver imaging was obtained with gamma camera. In other experiment, liver of treated rats was assessed for histopathology. Tc-phytate uptake (%ID/g) of livers in control, solvent, Vit E, MTX and Vit E + MTX groups were 8.99% ± 1.37, 8.53% ± 2.91, 8.65% ± 3.84, 3.22% ± 1.09 and 8.38% ± 2.68. Vit E administration resulted in a significant increase of the level of %ID/g in MTX-injected animal. Vit E pre-treatment improved the shape of liver in MTX-treated rat which was seen abnormal view in planar imaging. Histophatological examinations approved the protective effect of Vit E against MTX-induced hepatotoxicity in rats. The results of this study show that Vit E significantly attenuates the MTX-induced hepatotoxicity in rats, and 99m Tc-phytate is an acceptable radiopharmaceutical agent for assessment of liver and spleen damages in the animal model.

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“…It is metabolized to curcumin glucuronides, sulfates, tetrahydrocurcumin and hexacurcumin in the intestine and liver of human and rats and is reported to exhibit antioxidant, anti-inflammatory, choleretic, anti-carcinogenic, antiviral, and anti-infectious activities. [156,157] It inhibits the expression of the enzyme cyclooxygenase 2 via interference with activation of the transcription factor NF-κB and is a potential hepatoprotectant. [158,159] Some study showed the curcumin-mediated protection against paracetamol-induced liver damage by restoring antioxidant activity of liver.…”

Section: Curcuminmentioning

confidence: 99%

A Review on Drug Induced Hepatotoxicity and Its Management by Herbal Drugs

Singh¹

2017

WJPPS

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The liver plays vital functions in maintaining, performing and regulating the homeostasis of body and it involves in almost all biochemical pathways such as nutrient supply, reproduction, biotransformation, energy supply and growth. The liver metabolize, detoxify and activate exogenous as well as endogenous compound and is capable for transforming foreign chemicals as it has high level of enzymatic system. Liver disorder or injury is defined as conditions, diseases, or infections that influence the structure and/or function of

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Resveratrol ameliorates methotrexate-induced hepatotoxicity in rats via inhibition of lipid peroxidation

Cited by 107 publications

References 57 publications

Resveratrol Protects Sepsis-Induced Oxidative DNA Damage in Liver and Kidney of Rats

Resveratrol Protects Sepsis-Induced Oxidative DNA Damage in Liver and Kidney of Rats

The use of 99mTc-phytate for assessment the protective effect of vitamin E against hepatotoxicity induced by methotrexat in rat

A Review on Drug Induced Hepatotoxicity and Its Management by Herbal Drugs

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