Resveratrol Inhibits IgE-Mediated Basophilic Mast Cell Degranulation and Passive Cutaneous Anaphylaxis in Mice

Abstract

Resveratrol is a phytoalexin abundantly found in red grape skin and is effective in antitumor and antiinflammation associated with immune responses. This study investigated whether resveratrol suppressed immunoglobulin (Ig)E-mediated allergic responses and passive cutaneous anaphylaxis (PCA) in rat RBL-2H3 mast cells and in BALB/c mice. The release of β-hexosaminidase and histamine was enhanced in mast cells sensitized with anti-dinitrophenyl (DNP)-IgE and subsequently stimulated by DNP-human serum albumin (HSA), indicative of mast cell degranulation. When mast cells were pretreated with nontoxic resveratrol at 1-25 μmol/L, such induction was dose dependently diminished. Spleen tyrosine kinase (Syk) and phospholipase Cγ (PLCγ) of sensitized mast cells were activated by stimulation with DNP-HSA antigen, which was dampened by ≥5 μmol/L resveratrol. The phosphorylation of protein kinase C (PKC)μ and PKCθ was attenuated by administering resveratrol to DNP-HSA-exposed mast cells, whereas quiescent PKCζ/λ in sensitized cells was dose-dependently activated by resveratrol. Male BALB/c mice were sensitized for 24 h with DNP-IgE and orally administered with resveratrol 1 h before the DNP-HSA challenge. The histamine concentration was enhanced in sensitized mice challenged to DNP-HSA, which was reversed by administration of 10 mg/kg resveratrol. Additionally, it encumbered the tissue activation of Syk, PLCγ, and PKCμ in antigen-exposed mice. Resveratrol decreased IgE-mediated PCA and alleviated allergic edema of mouse ear and dorsal skin. Mast cell degranulation and allergic inflammation, accompanying the induction of monocyte chemotactic protein-1 and macrophage inflammatory protein-2, were inhibited by supplementing resveratrol to antigen-challenged mice. Resveratrol inhibited mast cell-derived, immediate-type allergic reactions, and these responses of resveratrol suggest possible therapeutic strategies in preventing allergic inflammatory diseases.

Imidacloprid inhibits IgE-mediated RBL-2H3 cell degranulation and passive cutaneous anaphylaxis

“…It was investigated to determine whether imidacloprid treatment could inhibit mast cell degranulation by measuring the release of β-hex and histamine from RBL-2H3 cells [31]. Fig.…”

The novel flavone tetramethoxyluteolin is a potent inhibitor of human mast cells

“…This method was previously used to document the effect of other inhibitors on MC activation 40 as well as of resveratrol on passive cutaneous anaphylaxis in mice 55 Moreover, the flavonoid concentrations used here in this mouse “model” (100 mg/kg) have already been shown to have a statistically significant benefit at least for lut in children with autism 56 many of whom have been reported to have “allergic-like” symptoms 57 implicating MC activation. 58 …”

Preliminary Clinical Effect Evaluation of Resveratrol in Adults with Allergic Rhinitis

“…In this study, we found that patients treated with resveratrol had significantly decreased IgE and IL-4 levels in the blood, suggesting that resveratrol inhibited IgE and IL-4 production. Han et al [29] reported that resveratrol inhibits IgE-mediated basophilic mast cell degranulation . This could be another mechanism that contributes to inhibition of AR.…”