Reticulated platelets as a screening test to identify thrombocytopenia aetiology

Monteagudo, Manuel; Amengual, M. J.; Muñoz, Luz; Soler, Joëlle; Roig, Inmaculada; Tolosa, Carlos

doi:10.1093/qjmed/hcn047

Cited by 35 publications

(29 citation statements)

References 26 publications

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“…We measured RP% using flow cytometry by a simple technique directly from whole-blood, avoiding extra manipulation, with a dual-labelling method for platelet identification. Monteagudo et al [24] who used the same protocol for measuring RP% as the one used in our study found that RP% value in excess of 11% had a high sensitivity and good specificity for a diagnosis of thrombocytopenia with increased thrombopoietic activity which reflected that RP% determination is a reliable platelet turnover marker. In the present study, the median RP% was significantly higher in thrombocytopenic versus non-thrombocytopenic patients (11.75% versus 2.72% respectively, P < 0.001).…”

Section: Discussionsupporting

confidence: 63%

“…However, there is an evident lack of analytical standardization as there is a significant variability in the various published protocols regarding the incubation time, the used substrate (whole blood or platelet-rich plasma), concentrations of the fluorescent dyes, and the use of a second antibody marker to distinguish platelets, which yielded much variation in the published reference intervals of reticulated platelets percent (RP%) [19] . Even so, numerous studies have suggested that analysis of RP represents a valuable estimate of the rate of platelet production in bone marrow [17,19,[20][21][22][23][24] .…”

Section: Introductionmentioning

confidence: 99%

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Reticulated Platelets in Egyptian Patients with Chronic Hepatitis C Infection

Boshnak¹

2017

jmscr

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Section: Discussionsupporting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Reticulated Platelets in Egyptian Patients with Chronic Hepatitis C Infection

Boshnak¹

2017

jmscr

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“…8 Furthermore, retPLTs are an early predictor of bone marrow recovery after chemotherapy and transplantation. 9 Therefore, a standardized and fully automated method would be desirable for routine use.…”

mentioning

confidence: 99%

Reference Intervals of Reticulated Platelets and Other Platelet Parameters and Their Associations

Hoffmann

Broek

Curvers

2013

Archives of Pathology &Amp; Laboratory Medicine

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Context.-Reticulated platelets are platelets recently released from the bone marrow, and they can serve as a noninvasive indicator of recent megakaryopoietic activity. Widespread clinical use has been hampered by laborious methods and lack of standardization. Recently, a fully automated method was released on the Abbott CELL-DYN Sapphire hematology analyzer.Objective.-To establish reference ranges for reticulated platelets. Secondary aims were to investigate associations between reticulated platelets and other platelet parameters like mean platelet volume, plateletcrit, and platelet distribution width.Design.-Reticulated platelets and other platelet parameters were measured in an unselected cohort of 8089 subjects visiting a primary health care laboratory. The reticulated platelet data were analyzed using the Bhattacharya technique. In addition, a nonparametric method was used in selected subjects with normal platelet counts for providing reference ranges.Results.-Reticulated platelets ranged from 0.4% to 6.0% or from 1 to 18 3 10 3 /L. Reticulated platelets increased significantly with the subjects' age. Statistically, males had slightly higher values than females, but the differences were negligible. Reticulated platelets were positively correlated with platelet count and negatively with mean platelet volume.Conclusions.-Reference ranges have been established for reticulated platelets as measured on the CELL-DYN Sapphire hematology analyzer. There were no relevant differences between the sexes, but there was a clear effect of age. An individual's reticulated platelets are associated with the platelet count as well as mean platelet volume.

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“…Bone marrow aspiration and biopsy should be performed when the aetiology is unclear. Reticulated platelets or the equivalent immature platelet fraction can help in discriminating between thrombocytopenia due to bone marrow failure (low percentages) and hyperdestructive thrombocytopenia (high percentages) [7,8], although the specificity of these tests has not been validated prospectively. Limited evidence suggests that plasma glycocalicin and thrombopoietin levels can increase the specificity of reticulated platelets in thrombocytopenia due to increased platelet destruction [9].…”

Section: Type Mechanisms Examplesmentioning

confidence: 99%

The Cardiac Related Thrombocytopenia

Sinan¹

2015

J Hematol Thrombo

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Thrombocytopenia is a common problem in cardiovascular patients. The aetiology and management may be different than those in other populations. The drugs that are used during percutaneous coronary interventions most commonly associated with thrombocytopenia are the glycoprotein (GP) IIb/IIIa receptor inhibitors and heparin. Thienopyridines rarely cause thrombocytopenia. The platelet count falls, primarily due to platelet damage and destruction in the bypass circuit and hemodilution after open-heart surgery. Heparin is the most common drug to be implicated in thrombocytopenia in intensive care unit patients. Determining the etiology for the low platelet count is important for the implementation of appropriate management. Heparin-induced thrombocytopenia is a potentially devastating immune mediated adverse drug reaction caused by the emergence of antibodies that activate platelets in the presence of heparin. Despite thrombocytopenia, bleeding is rare; rather, HIT is strongly associated with thromboembolic complications involving both the arterial and venous systems.

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Reticulated platelets as a screening test to identify thrombocytopenia aetiology

Cited by 35 publications

References 26 publications

Reticulated Platelets in Egyptian Patients with Chronic Hepatitis C Infection

Reticulated Platelets in Egyptian Patients with Chronic Hepatitis C Infection

Reference Intervals of Reticulated Platelets and Other Platelet Parameters and Their Associations

The Cardiac Related Thrombocytopenia

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