Reticuloendothelial system (RES) hyperfunction and erythropoietin (Ep) production in the regenerating liver

Naughton, Brian A.; Birnbach, David J.; Liu, P.; Kolks, Gail A.; Tung, Ming Z.; Piliero, Joseph A.; Piliero, Sam J.; Gordon, Albert S.

doi:10.1002/jso.2930120307

Cited by 17 publications

(6 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For several reasons, Epo was used in the present study in combination with Cur. First, Cur has been shown to suppress Epo production in tumour cell lines [41] and Epo production seems to be relevant during liver regeneration [30]. Second, besides its stimulatory effects on cellular proliferation, Epo also has antioxidant and anti-inflammatory effects [8,9,14] similar to that of Cur, making it a promising combination partner.…”

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Synergistic effect of erythropoietin but not G-CSF in combination with curcumin on impaired liver regeneration in rats

Seehofer

Neumann

Schirmeier

et al. 2008

Langenbecks Arch Surg

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 98%

“…It is known since many years that Epo [30] and G-CSF [31] are produced in the liver during regeneration after PH. However, Epo production seems to be more distinctive than G-CSF production [31].…”

Section: Discussionmentioning

confidence: 99%

Synergistic effect of erythropoietin but not G-CSF in combination with curcumin on impaired liver regeneration in rats

Seehofer

Neumann

Schirmeier

et al. 2008

Langenbecks Arch Surg

View full text Add to dashboard Cite

show abstract

“…PRL, similarly to GH and Epo (Naughton et al, 1977(Naughton et al, , 1979Pennisi et al, 2004;Conway-Campbell et al, 2007;Cui et al, 2007), regulates liver transcription factors involved in proliferation, metabolism and liver specific function. The results also show that the administration of a single PRL dose prior to hepatectomy accelerates the onset of liver regenerative machinery and consequently it has a liver protective role.…”

Section: Discussionmentioning

confidence: 99%

Prolactin's role in the early stages of liver regeneration in rats

Olazabal

Muñoz

Rodríguez-Navas

et al. 2009

Journal Cellular Physiology

View full text Add to dashboard Cite

Liver regeneration after partial hepatectomy (PHx) is a complex process that is regulated by hemodynamic changes, the modulation of cytokines and growth factors, and the activation of immediate early transcription factors that lead to a round of hepatocyte mitosis. Among the factors involved, the pituitary hormone prolactin (PRL) has been shown to induce a hepatotrophic response after partial hepatectomy similar to that caused by phorbol esters; and in isolated hepatocytes PRL triggers a mitogenic response. However, it is becoming clear that PRL exerts a dual role acting in proliferation and differentiation processes. In this work, we have assessed the role of PRL in the early stages of liver regeneration in rats. To this end, three groups of rats were compared: Sham operated, regenerant and regenerant with PRL i.p. administration. Results show that PRL administration prior to partial hepatectomy caused an increase in the binding activity of several transcription factors involved in cell proliferation: AP-1, c-Jun and STAT-3, and in liver-specific differentiation and maintenance of energetic metabolism: CEBPalpha, HNF-1, HNF-4 at early time points and at later time points HNF-3. Hepatic sections show that PRL administration increases the number of proliferating cells within 5 h post-partial hepatectomy. The mRNA of the angiogenic and survival factors VEGF and HIF-1alpha, was also induced by PRL treatment. Data indicate that PRL triggers, either directly or indirectly, an acceleration of liver regeneration, preserving liver function and fulfilling a hepatoprotective role. J. Cell. Physiol. 219: 626-633, 2009. (c) 2009 Wiley-Liss, Inc.

show abstract

“…In the fetus the liver is the primary source of Epo (2, 37), but the relative contribution of the Kupffer cell and parenchymal cell is unknown. Some evidence suggests that the Kupffer cell is a site of Epo production or storage or both (38)(39)(40)(41)(42). However, these studies did not directly quantitate the amount of Epo produced by Kupffer cells nor did they demonstrate regulated production of Epo by these cells.…”

Section: Discussionmentioning

confidence: 99%

The regulated expression of erythropoietin by two human hepatoma cell lines.

Goldberg

Glass²,

Cunningham³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

345

174

View full text Add to dashboard Cite

The development of a cell culture system that produces erythropoietin (Epo) in a regulated manner has been the focus of much effort. We have screened multiple renal and hepatic cell lines (including MDCK, LLC-PKI, BHK, WRL 68, CLCL, A704, CRFK, A498, ACHN, TCMK-1, LLC-MK2, CaKi-2, HepG2, and Hep3B) for either constitutive or regulated expression of Epo. Only the human hepatoma cell lines, Hep3B and HepG2, made significant amounts of Epo as measured both by radioimmunoassay and in vitro bioassay (as much as 330 milliunits per 10' cells in 24 hr It has been difficult to establish a cell culture system in which biologically active and immunologically identifiable Epo is produced in a regulated fashion by a single cell type. Many of the investigations cited above utilized tissues containing mixed cell types (5-9, 13). In other studies the demonstration of Epo production depended solely upon bioassays and/or inhibition of bioactivity by anti-Epo antibodies of questionable specificity (5-11, 13, 16, 19, 24). Some of the previously reported cell culture systems demonstrated only low levels of Epo production following stimulation by hypoxia or cobalt (II) (11,13,14,25), while still others possessed large amounts of constitutive Epo production (15)(16)(17)(18)(19)(20)(21)(22)(23) with little regulated production.In this report we demonstrate that, when grown at low cell density, two established human hepatoma cell lines can be induced to produce large amounts of biologically active and immunologically identifiable Epo in response to hypoxia or cobalt(II) chloride. Furthermore, upon such stimulation markedly increased levels of Epo mRNA were observed. MATERIALS AND METHODSCell Culture. All cell lines studied were obtained through the American Type Culture Collection. Cells were cultured in 100 x 20 mm tissue culture dishes (Coming no. 25020) and 25-cm2 tissue culture flasks (Coming no. 25100) using a minimal essential medium (GIBCO) supplemented with glutamine, penicillin (100 units per ml), streptomycin (100 /Lg/ml), and 10% heat-inactivated (560C for 30 min) fetal calf serum (Hazelton Research Products, Denver, PA). Cells were maintained in a humidified 5% C02/95% air incubator at 370C. The culture medium was changed daily for 1 or 2 days prior to all experiments. In experiments in which the oxygen tension was varied, the 25-cm2 flasks were fitted with rubber septa. The gas inlet was provided through a 9 cm 18 g needle, fitted with a 0.2-,Lm sterile filter, and the gas outlet was provided through a 4 cm 18 g needle connected by Silastic tubing to a closed water trap. Needles, tubing, and septa were autoclaved prior to use, and assembly was performed under sterile conditions. Experimental gases containing 1%, 2%, 3%, 5%, or 21% 02, 5% C02, and the balance N2 (Yankee Oxygen) were hydrated at 37°C and flowed at a rate of2.4-4.2 ml/min into the tissue culture flasks. The flasks were immersed in a 37°C constant-temperature water bath. Po2 and Abbreviations: Epo, erythropoietin; PGE2, prostaglandin E2. 7972The publica...

show abstract

Reticuloendothelial system (RES) hyperfunction and erythropoietin (Ep) production in the regenerating liver

Cited by 17 publications

References 19 publications

Synergistic effect of erythropoietin but not G-CSF in combination with curcumin on impaired liver regeneration in rats

Synergistic effect of erythropoietin but not G-CSF in combination with curcumin on impaired liver regeneration in rats

Prolactin's role in the early stages of liver regeneration in rats

The regulated expression of erythropoietin by two human hepatoma cell lines.

Contact Info

Product

Resources

About