Synergistic effect of erythropoietin but not G-CSF in combination with curcumin on impaired liver regeneration in rats

Abstract

In the present model, Epo, at a relatively low dosage, did not improve liver regeneration. However, the combination of Epo and Cur showed a synergistic effect with highly significant stimulation of liver regeneration.

Effect of Erythropoietin on Oxidative Stress and Liver Injury in Experimental Obstructive Jaundice

“…In a recent study Seehofer et al [35] showed that Epo reduces the anti-inflammatory response and oxidative stress and attenuates liver damage in a rat model with liver resection, similar to our findings. In another study, Tascilar et al [39] showed that Epo can attenuate acute necrotizing pancreatitis-induced lung injury by inhibiting polymorphonuclear cytokines, preserving microvascular endothelial cell integrity and reducing oxidative stress-associated lipid peroxidation.…”

“…Decreased GSH levels in the liver tissue and circulation in obstructive jaundice have been shown in previous studies [35] . GSH levels decrease because GSH turns into oxidized glutathione and the GSH/oxidized glutathione ratio decreases in cholestatic liver.…”

“…Moreover, MDA levels in both Epo groups (Epo-3 and Epo-7) were significantly lower than in the Non-Epo groups. These findings indicate that Epo attenuates oxidative injury, which has been proved to have occurred in obstructive jaundice in previous studies [35,38] . Parallel with the MDA levels, the Non-Epo groups had the highest NO levels amongst the CBDL groups, and this elevation was also significantly different to the sham and Epo groups.…”

Inhibitory Effect of Curcumin on Early Liver Regeneration Following Partial Hepatectomy in Rats

“…Also in the present model hypertrophy of hepatocytes had to be considered, but the DNA-synthesis was maintained. However, in a distinct experimental setup, using a rat model of delayed liver regeneration, it could be shown that curcumin not as monotherapy but in combination with erythropoietin could significantly stimulate hepatocellular proliferation [20].…”

Multidrug donor preconditioning prevents cold liver preservation and reperfusion injury

“…With the aim to achieve the best possible organ protection by adjunct preconditioning, we included those drugs in our MDDP protocol, which have been shown in previous single-drug preconditioning studies to be successful in attenuating postischemic liver injury. These substances provide protection be exerting anti-oxidative, anti-inflammatory, and anti-apoptotic actions, or may even be characterized by a pleiotropic action profile [52], such as simvastatin [42], glycine [53,54], erythropoietin [55], and methylprednisolone [56]. The data of the present study now indicate that MDDP is capable of almost completely preventing cold I/R injury.…”