RETRACTED ARTICLE: Effect of RAB31 silencing on osteosarcoma cell proliferation and migration through the Hedgehog signaling pathway

Yu, Qiong; Li, Dong; Wang, Dan; Hu, Chunmei; Sun, Yan; Tang, Yan; Shi, Guang

doi:10.1007/s00774-018-0961-9

Cited by 7 publications

(5 citation statements)

References 39 publications

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“…38 Furthermore, RAB31 can facilitate osteosarcoma cell proliferation and migration through regulating the Hedgehog signaling pathway. 39 In our research, RAB31 was confirmed to be the target gene of miR-582-3p. We also proved the upregulation of RAB31 in NPC cells and it promoted cell proliferation and migration.…”

Section: Discussionsupporting

confidence: 53%

LncRNA HOXA10-AS Activated by E2F1 Facilitates Proliferation and Migration of Nasopharyngeal Carcinoma Cells Through Sponging miR-582-3p to Upregulate RAB31

Wang

Nie

Zhu

2022

Am J Rhinol�Allergy

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Nasopharyngeal carcinoma (NPC) is a kind of head and neck cancer with a characteristic regional distribution. Increasing evidence has illustrated that long noncoding RNAs (lncRNAs) exert the regulatory function in tumor development. Nevertheless, the specific functions of lncRNA HOXA10 antisense RNA (HOXA10-AS) in NPC remain to be clarified. In this research, quantitative reverse transcription polymerase chain reaction detected HOXA10-AS expression in NPC cells. Cell counting kit-8, colony formation, and transwell assays were utilized to measure the proliferation and migration of NPC cells. Moreover, mechanism assays detected the interaction of different genes. Briefly, HOXA10-AS was highly expressed in NPC cells. HOXA10-AS down-regulation restrained NPC cell proliferation and migration. Further, HOXA10-AS could bind to miR-582-3p by acting as a competing endogenous RNA. Besides, Ras-related protein Rab-31 (RAB31) was proven as the target gene of miR-582-3p. Additionally, E2F transcription factor 1 (E2F1) acted as a transcription factor to activate HOXA10-AS expression. In the final rescue assays, we observed that the effect of HOXA10-AS depletion on NPC cell growth could be fully reversed by RAB31 overexpression or miR-582-3p inhibition. In short, our research proved that HOXA10-AS activated by E2F1 facilitated proliferation and migration of NPC cells through sponging miR-582-3p to upregulate RAB31.

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Section: Discussionsupporting

confidence: 53%

LncRNA HOXA10-AS Activated by E2F1 Facilitates Proliferation and Migration of Nasopharyngeal Carcinoma Cells Through Sponging miR-582-3p to Upregulate RAB31

Wang

Nie

Zhu

2022

Am J Rhinol�Allergy

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“…Rab31 is highly expressed in breast cancer [16], gastric cancer [21], pancreatic cancer [19] and other malignancies. Additionally, Rab31 promotes the proliferation and metastasis of osteosarcoma cells [20]. We showed that Rab31 level was higher in HPV-positive cervical cancer cells than in HPV-negative cervical cancer cells.…”

Section: Discussionmentioning

confidence: 72%

“…Elevated Rab31 in pancreatic cancer is associated with reduced overall survival [19]. Rab31 is related with the malignant behavior of gastric cancer and osteosarcoma [20,21]. Recently, our mass spectrometry results show that Rab31 expression is upregulated in HPV16 E6-or E7-expressing cells (data not shown).…”

Section: Ivyspring International Publishermentioning

confidence: 80%

“…The Hedgehog signaling pathway regulates the differentiation of human tissues/organs and is implicated in multiple cancers [24]. Studies have shown that the Hedgehog signaling pathway is upregulated in osteosarcoma [25] and that Rab31 knockdown reduces the invasion capacity of osteosarcoma cells via inhibition of the Hedgehog signaling pathway [20]. Hepatocyte growth factor (HGF), which is secreted predominantly by cancer-associated fibroblasts and present in the tumor microenvironment, promotes the survival and migration of cancer cells [26].…”

Section: Rab31 Promotes the Migration And Invasion Of Cervical Cancer Cells Via Mapk6mentioning

confidence: 99%

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Rab31 promotes the invasion and metastasis of cervical cancer cells by inhibiting MAPK6 degradation

Huang¹,

Liu²,

Han³

et al. 2022

Int. J. Biol. Sci.

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“…Similarly, Jones et al (2012) reported a subset of miRNA, miR‐126, miR‐142‐5p, miR‐195, miR‐223, and miR‐451 to be downregulated in OS cells compared to osteoblasts. Meanwhile, RAB31 was found to participate in the disease progression of OS and its silencing suppressed the OS progression (Yu et al, 2019). Thereby, we then downregulated miR‐1286 expression in OS cell lines and as predicted, we found that the inhibition of miR‐1286 could reverse the GANT61‐induced cell inhibition.…”

Section: Discussionmentioning

confidence: 99%

GANT61 plays antitumor effects by inducing oxidative stress through the miRNA‐1286/RAB31 axis in osteosarcoma

Zhang

Chu

2020

Cell Biology International

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Osteosarcoma (OS) is a rare malignancy of bone associated with poor clinical outcomes. The antitumor effects of GANT61 on OS is unclear. To investigate antitumor effects and mechanism of GANT61 in OS cells and xenograft model. Effects of GANT61 on cell viability, clone formation, cell cycle, apoptosis, migration, and invasion ability of OS cells were assessed. Reactive oxygen species (ROS) levels measured by dichlorofluorescein fluorescence were used to evaluate oxidative stress. The Xenograft model was constructed to investigate the antitumor effects of GANT61 in vivo. The microRNA (miRNA)‐1286 was downregulated, while RAB31 upregulated in OS tissues and cells. GANT61 inhibited viability, migration, and invasion ability of OS cells (SaOS‐2 and U2OS), and induced apoptosis and the ROS production, along with miRNA‐1286 upregulation and RAB13 downregulation. After knockdown of miRNA‐1286, GANT6‐induced cell inhibition was attenuated, along with RAB31 upregulation. Inversely, miRNA‐1286 overexpression downregulated RAB31. Dual‐luciferase reporter assay verified that miR‐1286 negatively targeted RAB13. Moreover, the knockdown of RAB31 stimulated apoptosis and ROS production while inhibited viability, migration, and invasion of GANT61‐treated cells. In vivo experiments further confirmed that GANT61 inhibited tumor growth and RAB13 expression, but enhanced miRNA‐1286. The study demonstrated that GANT61 inhibited cell aggressive phenotype and tumor growth by inducing oxidative stress through the miRNA‐1286/RAB31 axis. Our findings provided a potential antitumor agent for the OS clinical treatment.

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RETRACTED ARTICLE: Effect of RAB31 silencing on osteosarcoma cell proliferation and migration through the Hedgehog signaling pathway

Cited by 7 publications

References 39 publications

LncRNA HOXA10-AS Activated by E2F1 Facilitates Proliferation and Migration of Nasopharyngeal Carcinoma Cells Through Sponging miR-582-3p to Upregulate RAB31

LncRNA HOXA10-AS Activated by E2F1 Facilitates Proliferation and Migration of Nasopharyngeal Carcinoma Cells Through Sponging miR-582-3p to Upregulate RAB31

Rab31 promotes the invasion and metastasis of cervical cancer cells by inhibiting MAPK6 degradation

GANT61 plays antitumor effects by inducing oxidative stress through the miRNA‐1286/RAB31 axis in osteosarcoma

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