RETRACTED ARTICLE: Impact of ultrasonication techniques on the preparation of novel Amiloride-nanoemulsion used for intranasal delivery in the treatment of epilepsy

Ahmad, Niyaz; Ahmad, Rizwan; Alam, Md. Aftab; Ahmad, Farhan Jalees; Amir, Mohd

doi:10.1080/21691401.2018.1489826

Cited by 40 publications

(27 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A four-factor, three-level central composite experimental design (CCD) [21] was employed for the optimization and evaluation of the correlation between the independent variables like PLGA (X 1 ), PVA (X 2 ), sonication time (X 3 ), and temperature (X 4 ), and the dependent (responses) variables including particle size (Y 1 ), polydispersity index (PDI) (Y 2 ), and zeta potential (Y 3 ) ( Table 1). On the other side for the prediction of better response, CCD is better as compared with the Box-Benhnken design (BBD) [21], as BBD suggested formulation only, that is, independent variables' values (lower, middle, and higher). CCD, however, included two additional values, (+α) and (-α), in addition to the above-mentioned values in which the rotatability necessities of the design are enclosed [21].…”

Section: Optimization By Central Composite Designmentioning

confidence: 99%

“…On the other side for the prediction of better response, CCD is better as compared with the Box-Benhnken design (BBD) [21], as BBD suggested formulation only, that is, independent variables' values (lower, middle, and higher). CCD, however, included two additional values, (+α) and (-α), in addition to the above-mentioned values in which the rotatability necessities of the design are enclosed [21]. As per the experimental runs, the preparation of a total of 30 formulations was constructed ( Table 2).…”

Section: Optimization By Central Composite Designmentioning

confidence: 99%

“…DSC (Differential Scanning Calorimetry) Study DSC 214 Polyma (NETZSCH-Wittelsbacherstraße 42, 95100 Selb, Germany) was used to determine the DSC of pure catechin (CH); polymer (PLGA); poly vinyl alcohol (PVA); a physical mixture of CH, PVA, and PLGA; freeze-dried-CH-loaded-optimized polymer (PLGA)-NPs; pure chitosan; and CS-coated-CH-loaded-optimized polymer (PLGA)-NPs. The sample (10 mg) was kept inside a standard aluminium pan, crimped, and heated (20.0 • C to 400.0 • C) at a rate of 10.0 • K/min, followed by a continuous supply of nitrogen [21,28,34].…”

Section: Loading Capacity (%Lc) Entrapment Efficiency (%Ee) and %Prmentioning

confidence: 99%

“…The dose for each rat based their body weight (70.0 mg/kg PTZ) was given intra-peritoneal, and they were observed for half an hour for myoclonic jerks as well as for clonic generalized seizures, which were noted [21,47]. For the development of PTZ seizure, we divided the rats into four groups as follows: Group 1 (normal saline treated/control group), Group 2 (catechin hydrate simple suspension treated), Group 3 (CH-PLGA-NPs treated), and Group 4 (CS-coated-CH-loaded-PLGA-NPs treated).…”

Section: Seizures Developed By Ptzmentioning

confidence: 99%

“…Many researchers are focusing on nose-to-brain drug delivery systems as they have many advantages such as hepatic first pass metabolism, avoiding the blood brain barrier (BBB), and being a non-invasive route with easy administration of drugs [21][22][23]. Before designing the intranasal preparations, it is important to understand the problem of the short-stay time of the drug in the nasal cavity.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Quantification and Evaluations of Catechin Hydrate Polymeric Nanoparticles Used in Brain Targeting for the Treatment of Epilepsy

et al. 2020

Self Cite

View full text Add to dashboard Cite

To formulate novel chitosan (CS)-coated–PLGA–nanoparticles (NPs) using a central composite design approach and use them in order to improve brain bioavailability for catechin hydrate (CH) through direct nose-to-central nervous system (CNS) delivery for the evaluation of a comparative biodistribution study of CH by the newly developed ultra high performance liquid chromatography mass spectroscopy and mass spectroscopy (UHPLC-MS/MS) method in the treatment of epilepsy. For PLGA–NPs’ preparation, a double emulsion-solvent evaporation method was used, where a four-factor, three-level central composite design was used to obtain the best nanoformulation. For the optimization, four independent variables were chosen, that is, PLGA, polyvinyl alcohol (PVA), sonication time, and temperature. The optimized PLGA–NPs were further coated with chitosan and assessed for drug release, nasal permeation study, as well as a comparative pharmacokinetic and pharmacodynamic study. Independent and dependent variables helped to optimize the best nanoformulation based on the composition of PLGA (50.0 mg), PVA (1.10%), sonication time (90.0 s), and temperature (25.0 °C). The values of dependent variables were observed, such as polydispersity index (PDI), particle size, and zeta potential (ZP)—that is, 0.106 ± 0.01, 93.46 ± 3.94 nm, and −12.63 ± 0.08 mV, respectively. The ZPs of CS-coated PLGA–NPs were changed from negative to positive value with some alteration in the distribution of particle size. Excellent mucoadhesive-nature of CS–CH–PLGA–NPs as compared with CH–S and CH–PLGA–NPs was seen, with a retention time of 0.856 min and m/z of 289.23/245.20 for CH, together with a retention time of 1.04 min and m/z of 301.21/151.21 for Quercetin as an internal standard (IS). For a linear range (1–1000 ng mL−1), % accuracy (93.07–99.41%) and inter- and intraday % precision (0.39–4.90%) were determined. The improved Cmax with area under curve (AUC)0–24 was found to be highly significant (p < 0.001) in Wistar rats’ brain as compared with the i.n. and i.v. treated group based on the pharmacokinetics (PK) results. Furthermore, CS–CH–PLGA–NPs were found to be more significant (p < 0.001) for the treatment of seizure threshold rodent models, that is, increasing current electroshock and pentylenetetrazole-induced seizures. A significant role of CS–CH–PLGA–NPs was observed, that is, p < 0.001, for the enhancement of brain bioavailability and the treatment of epilepsy.

show abstract

Section: Optimization By Central Composite Designmentioning

confidence: 99%

Section: Optimization By Central Composite Designmentioning

confidence: 99%

Section: Loading Capacity (%Lc) Entrapment Efficiency (%Ee) and %Prmentioning

confidence: 99%

Section: Seizures Developed By Ptzmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Quantification and Evaluations of Catechin Hydrate Polymeric Nanoparticles Used in Brain Targeting for the Treatment of Epilepsy

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

Lipid Nanoformulation of Nutraceuticals as Neurotherapeuticals in Neurological Disorders

Taleuzzaman¹,

Chauhan²,

Tomar³

et al. 2022

Nanotechnology in Functional Foods

View full text Add to dashboard Cite

Molecular mechanisms of anticancer effect of rutin

Imani

Maleki

Bohlouli

et al. 2020

Phytotherapy Research

130

View full text Add to dashboard Cite

Because of the extensive biological functions of natural substances such as bioflavonoids, and their high safety and low costs, they could have high priority application in the health care system. The antioxidant properties of rutin, a polyphenolic bioflavonoid, have been well documented and demonstrated a wide range of pharmacological applications in cancer research. Since chemotherapeutic drugs have a wide range of side effects and rutin is a safe anticancer agent with minor side effects so recent investigations are performed for study of mechanisms of its anticancer effect.Both in-vivo and in-vitro examinations on anticancer mechanisms of this natural agent have been widely carried out. Regulation of different cellular signaling pathways such as Wnt/β-catenin, p53-independent pathway, PI3K/Akt, JAK/STAT, MAPK, p53, apoptosis as well as NF-ĸB signaling pathways helps to mediate the anticancer impacts of this agent. This study tried to review the molecular mechanisms of rutin anticancer effect on various types of cancer. Deep exploration of these anticancer mechanisms can facilitate the development of this beneficial compound for its application in the treatment of different cancers.

show abstract

RETRACTED ARTICLE: Impact of ultrasonication techniques on the preparation of novel Amiloride-nanoemulsion used for intranasal delivery in the treatment of epilepsy

Abstract: A significant role of Amilo-NE as observed after high targeting potential and efficiency of the formulation supports the easy brain targeting for Amilo-NE.

Cited by 40 publications

References 37 publications

Quantification and Evaluations of Catechin Hydrate Polymeric Nanoparticles Used in Brain Targeting for the Treatment of Epilepsy

Quantification and Evaluations of Catechin Hydrate Polymeric Nanoparticles Used in Brain Targeting for the Treatment of Epilepsy

Lipid Nanoformulation of Nutraceuticals as Neurotherapeuticals in Neurological Disorders

Molecular mechanisms of anticancer effect of rutin

Contact Info

Product

Resources

About