RETRACTED ARTICLE: miR-506 contributes to malignancy of cutaneous squamous cell carcinoma via targeting of P65 and LAMC1

Abstract

Previous research has shown that microRNA 506 (miR-506) functions as an essential modulator in the development of many biological reactions, including multiple cancers. However, its involvement in cutaneous squamous cell carcinoma (CSCC) has been rarely reported. In the present work, we investigated the molecular mechanism and function of miR-506 in the regulation of CSCC cell viability and metastasis (migration and invasion). We observed that miR-506 expression was upregulated in both CSCC tissues and cell lines, and that decreased miR-506 expression led to repressed tumorigenesis in CSCC cells. Furthermore, flow cytometry revealed that the depletion of miR-506 resulted in decreased proliferation and increased apoptotic levels in CSCC cells. Meanwhile, it was found that miR-506 decreased CSCC cell migration and invasion in vitro. The dual-luciferase reporter assay also revealed that miR-506 targets the 3′-UTRs of p65 and Laminin C1 (LAMC1) for silencing. Silencing of p65 expression counteracted the pro-apoptotic influence of miR-506 depletion in CSCC cells, while inhibition of LAMC1 expression restored the migration and invasion properties of the CSCC cells. Therefore, the results provide evidence for the need to probe the biological and molecular mechanisms behind the development and progression of CSCC and may lead to novel treatment CSCC strategies.

MicroRNA-573 inhibits cell proliferation, migration and invasion and is downregulated by PICSAR in cutaneous squamous cell carcinoma

“…Similarly, in cSCC, some miRNAs expressions have been reported to be dysregulated and play crucial roles in tumorigenesis and development in cSCC. For instance, Zhou et al demonstrated that miR-506 expression was upregulated in both cSCC tissues and cell lines and downregulation of miR-506 expression repressed tumorigenesis in cSCC cells by targeting P65 and LAMC1 [ 20 ]. The miR-217 expression, which was upregulated in the cSCC cell lines and was found to promote cSCC cell growth, cell cycle and invasion, contributed to the development of cSCC [ 21 ].…”

Comprehensive Analysis of the Expression and Prognosis for Laminin Genes in Ovarian Cancer

“…According to Zhang et al, LAMC1 overexpression indicates poor prognosis in hepatocellular carcinoma (HCC)[ 33 ]. LAMC1 overexpression also indicated poor prognosis for cutaneous squamous cell carcinoma[ 34 ] and prostate cancer[ 35 ]. LAMC1 may participate in HCC progression by regulating PKM2 expression via the PTEN/AKT pathway[ 6 ].…”

Importance of microRNAs in Skin Oncogenesis and Their Suitability as Agents and Targets for Topical Therapy

“…Gong et al [47] reported that miR-221 promotes cell proliferation and colony growth by repressing PTEN. Furthermore, it was shown that inhibition of miR-506 induces apoptosis and decreases invasion and migration of cSCC cell lines [49]. In this study, LAMC1 and p65 were identified as direct targets of miR-506.…”

“…Functional analyses show that miR-365 inhibits apoptosis by directly targeting BAX [44] and forcing the cell cycle progression by NFIB repression [42, 43]. As shown in recent studies, miR-186, miR-221, miR-506, and miR-142-5p contribute to cSCC tumor growth [45-49]. miR-186 promotes proliferation and inhibits apoptosis by targeting RETREG1 [48] and APAF1 [46].…”