RETRACTED: LncRNA FEZF1-AS1 Modulates Cancer Stem Cell Properties of Human Gastric Cancer Through miR-363-3p/HMGA2

Hui, Yuanjian; Yang, Yan; Li, Deping; Wang, Juan; Di, Maojun; Zhang, Shichao; Wang, Shasha

doi:10.1177/0963689720925059

Cited by 24 publications

(15 citation statements)

References 56 publications

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“…The luciferase assay verified that miR ‐ 363 ‐ 3p could bind to the 3′‐UTR of CELF2 . It has been reported that miR ‐ 363 ‐ 3p is involved in multiple processes during tumour development 27‐29 . We performed a series of experiments to confirm biological functions of miR ‐ 363 ‐ 3p .…”

Section: Discussionmentioning

confidence: 98%

miR‐363‐3p induces EMT via the Wnt/β‐catenin pathway in glioma cells by targeting CELF2

Fan

Su²,

Song

et al. 2021

J Cellular Molecular Medi

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In our previous study, we reported that CELF2 has a tumour‐suppressive function in glioma. Here, we performed additional experiments to elucidate better its role in cancer. The expression profile of CELF2 was analysed by the GEPIA database, and Kaplan–Meier curves were used to evaluate the overall survival rates. Four different online databases were used to predict miRNAs targeting CELF2, and the luciferase assay was performed to identify the binding site. The biological effects of miR‐363‐3p and CELF2 were also investigated in vitro using MTT, Transwell, and flow cytometry assays. Western blotting, qPCR, and TOP/FOP flash dual‐luciferase assays were performed to investigate the impact of miR‐363‐3p and CELF2 on epithelial‐to‐mesenchymal transition (EMT) and the Wnt/β‐catenin pathway. The effect of miR‐363‐3p was also tested in vivo using a xenograft mouse model. We observed an abnormal expression pattern of CELF2 in glioma cells, and higher CELF2 expression correlated with better prognosis. We identified miR‐363‐3p as an upstream regulator of CELF2 and confirmed its direct binding to the 3′‐untranslated region of CELF2. Cell function experiments showed that miR‐363‐3p affected multiple aspects of glioma cells. Suppressing miR‐363‐3p expression inhibited glioma cell proliferation and invasion, as well as promoted cell death via attenuating EMT and blocking the Wnt/β‐catenin pathway. These effects could be abolished by the downregulation of CELF2. Treatment with ASO‐miR‐363‐3p decreased tumour size and weight in nude mice. In conclusion, miR‐363‐3p induced the EMT, which resulted in increased migration and invasion and reduced apoptosis in glioma cell lines, via the Wnt/β‐catenin pathway by targeting CELF2.

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Section: Discussionmentioning

confidence: 98%

miR‐363‐3p induces EMT via the Wnt/β‐catenin pathway in glioma cells by targeting CELF2

Fan

Su²,

Song

et al. 2021

J Cellular Molecular Medi

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“…Silencing of lncRNA HEIH inhibits liver cancer cell growth and metastasis through miR-199a-3p/mTOR axis [ 29 ]. Hui et al have found that FEZF1-AS1 modulates cancer stem cell properties through miR-363-3p/HMGA2 axis in GC [ 30 ]. LncRNA DCST1-AS1 has proved that it could regulate cell proliferation and apoptosis in GC by targeting miR-605-3p [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

LncRNA SNHG15 modulates gastric cancer tumorigenesis by impairing miR-506-5p expression

Chen

Zhong

et al. 2021

Bioscience Reports

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The gastric cancer (GC) patients commonly have a poor prognosis due to its invasiveness and distant metastasis. Growing evidence proved that aberrant long non-coding RNAs (lncRNAs) expression contributes to tumor development and progression. LncRNA SNHG15 has been reported to be involved in many different kinds of cancer, while its role in GC remains unclear. In the present study, we found that SNHG15 was up-regulated in GC tissues and cell lines. Silencing SNHG15 suppressed proliferation migration, invasion and promoted apoptosis of AGS cells. More importantly, microRNA-506-5p (miR-506-5p) was predicted as a direct target of SNHG15 by binding its 3’-UTR and further verified using luciferase reporter assay. Meanwhile, the results of rescue experiments revealed that knockdown of miR-506-5p expression reversed the functional effects of SNHG15 silenced on cell proliferation, migration, invasion and apoptosis. In conclusion, our findings revealed that SNHG15 executed oncogenic properties in GC progression through targeting miR-506-5p, which might provide a novel target for the GC treatment.

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“…GC-derived exosomes carrying miR-107 are able to promote MDSC expansion and activation via targeting DICER1 and PTEN, therefore suppressing tumor growth and augmenting immunotherapy efficacy (Ren et al, 2019). Recently, Zhang et al revealed that EVs loaded with miR-130b-3p mediate communication between GC cells and M2 macrophages in the tumor microenvironment by modulating MLL3 and GRHL2 (Zhang Y. et al, 2020). According to data from the TCGA database, lncRNA RP11-323N12.5 is identified as the most significantly upregulated lncRNA in GC.…”

Section: Tumor Immune Escapementioning

confidence: 99%

Non-Coding RNAs in Gastric Cancer: From Malignant Hallmarks to Clinical Applications

Chen

Shuai

et al. 2021

Front. Cell Dev. Biol.

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Gastric cancer (GC) is one of the most lethal malignancies worldwide. However, the molecular mechanisms underlying gastric carcinogenesis remain largely unknown. Over the past decades, advances in RNA-sequencing techniques have greatly facilitated the identification of various non-coding RNAs (ncRNAs) in cancer cells, including microRNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs). Accumulating evidence has revealed that ncRNAs are essential regulators in GC occurrence and development. However, ncRNAs represent an emerging field of cancer research, and their complex functionality remains to be clarified. Considering the lack of viable biomarkers and therapeutic targets in GC, further studies should focus on elucidating the intricate relationships between ncRNAs and GC, which can be translated into clinical practice. In this review, we summarize recent research progress on how ncRNAs modulate the malignant hallmarks of GC, especially in tumor immune escape, drug resistance, and stemness. We also discuss the promising applications of ncRNAs as diagnostic biomarkers and therapeutic targets in GC, aiming to validate their practical value for clinical treatment.

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RETRACTED: LncRNA FEZF1-AS1 Modulates Cancer Stem Cell Properties of Human Gastric Cancer Through miR-363-3p/HMGA2

Cited by 24 publications

References 56 publications

miR‐363‐3p induces EMT via the Wnt/β‐catenin pathway in glioma cells by targeting CELF2

miR‐363‐3p induces EMT via the Wnt/β‐catenin pathway in glioma cells by targeting CELF2

LncRNA SNHG15 modulates gastric cancer tumorigenesis by impairing miR-506-5p expression

Non-Coding RNAs in Gastric Cancer: From Malignant Hallmarks to Clinical Applications

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