RETRACTED: Thymoquinone poly (lactide-co-glycolide) nanoparticles exhibit enhanced anti-proliferative, anti-inflammatory, and chemosensitization potential

Ravindran, Jayaraj; Nair, Hareesh B.; Sung, Bokyung; Prasad, Sahdeo; Tekmal, Rajeshwar Rao; Aggarwal, Bharat B.

doi:10.1016/j.bcp.2010.01.023

Cited by 132 publications

(77 citation statements)

References 39 publications

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“…The principal phytochemicals of NSSEO are thymoquinone, p-cymene, α-pinene, thymohydroquinone, dithymoquinone and nigellone (Al Juhaimi et al, 2013). In fact, thymoquinone formed the main part of NSSEO to which mostly NSSEO therapeutic usages are attributed to it (Ravindran et al, 2010). It is reported that the production of inflammatory cytokines can be inhibited by thymoquinone in NSSEO (Ahmad et al, 2013).…”

Section: Introduction:-mentioning

confidence: 99%

Topical Co-Delivery of Indomethacin and Nigella Sativa L. Essential Oil in Poly- -Caprolactone Nanoparticles: In Vivo Study of Anti-Inflammatory Activity.

Badri¹,

Mohamed²,

Affendi³

et al. 2018

IJAR

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Indomethacin is a potent, nonselective Non-steroidal Antiinflammatory Drug (NSAID) but its low water-solubility precludes its use as topical dosage form. As with other NSAIDs, the systemic delivery is associated with high risk of serious gastrointestinal adverse events including bleeding, ulceration and perforation of stomach and intestines. Here we demonstrate a safer way of administration i.e via topical demonstrating synergistic effects when co-delivered with Nigella sativa L. seeds essential oil (NSSEO) in the form of coencapsulated particles (~200 nm) of poly--caprolactone. The particles showed penetrability across stratum corneum to dermis layer in ex-vivo human skin. Further study in the xyline-induced ear edema in mice was performed, and co-encapsulated particles demonstrated highest antiinflammatory effect compared to indomethacin particles and indomethacin gels. Despite slower onset compared to indomethacin gels, the inflamed ear continued to show reduction in thickness over 8 hours of observation demonstrating synergistic and pro-longed effect contributed by NSSEO. In immunohistochemistry study of CD45+, the mice ears treated with co-encapsulated particles showed considerable reduction in lesions, epidermal-dermal separation and inflammatory cells (lymphocytes and neutrophils) infiltration as compared to other formulation. Based on microscopic evaluation, the anti-inflammatory inhibition effect of co-encapsulated particles is the highest (90%) followed by indomethacin particles (79%) and indomethacin gel (49%). The findings suggest not only skin permeability of indomethacin significantly improved but also the therapeutic effects, all provided by the presence of NSSEO in the particles. This study paves the way to

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Section: Introduction:-mentioning

confidence: 99%

Topical Co-Delivery of Indomethacin and Nigella Sativa L. Essential Oil in Poly- -Caprolactone Nanoparticles: In Vivo Study of Anti-Inflammatory Activity.

Badri¹,

Mohamed²,

Affendi³

et al. 2018

IJAR

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“…In a previous study, Ravindran et al, (2010) succeeded in loading TQ into a poly (lactide-co-glycolide) nanoparticle. Other attractive nanocarriers are liposomes.…”

Section: Original Articlementioning

confidence: 99%

Thymoquinone in liposomes: a study of loading efficiency and biological activity towards breast cancer

et al. 2012

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“…Treatment of TQ at 10 mM induced inhibition of cell growth by 15% compared with the control (Ravindran et al 2010). In contrast to MDA-MB-231, MCF-7 has an estrogen receptor and expresses wild-type p53, a tumor-suppressor protein.…”

Section: Discussionmentioning

confidence: 92%

Thymoquinone (TQ) regulates cyclooxygenase-2 expression and prostaglandin E2 production through PI3kinase (PI3K)/p38 kinase pathway in human breast cancer cell line, MDA-MB-231

Yu¹,

Kim²

2012

Animal Cells and Systems

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Thymoquinone (TQ), a drug extracted from the black seeds of Nigella sativa, has been shown to exhibit antiinflammatory, anti-oxidant, and anti-neoplastic effects in numerous cancer cells. The effects of TQ on cyclooxygenase-2 (COX-2) expression and prostaglandin E 2 (PGE 2 ) production in MDA-MB-231, however, remain poorly understood. Western blot analysis and immunofluorescence staining were performed to study the expression levels of inflammation regulatory proteins in MDA-MB-231. PGE 2 assay was conducted to explore the TQ-induced production of PGE 2 . In this study, we investigated the effects of TQ on COX-2 expression and PGE 2 production in MDA-MB-231. TQ significantly induced COX-2 expression and increased PGE 2 production in a dose-dependent manner, as determined by a Western blot analysis and PGE 2 assay. Furthermore, the activation of Akt and p38 kinase, respectively, was up-regulated in TQ treated cells. Inhibition of p38 kinase with SB203580 and PI3kinase (PI3K) with LY294002 abolished TQ-caused COX-2 expression and decreased PGE 2 production. These results collectively demonstrate that TQ effectively modulates COX-2 expression and PGE 2 production via PI3K and p38 kinase pathways in the human breast cancer cell line MDA-MB-231.

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RETRACTED: Thymoquinone poly (lactide-co-glycolide) nanoparticles exhibit enhanced anti-proliferative, anti-inflammatory, and chemosensitization potential

Cited by 132 publications

References 39 publications

Topical Co-Delivery of Indomethacin and Nigella Sativa L. Essential Oil in Poly- -Caprolactone Nanoparticles: In Vivo Study of Anti-Inflammatory Activity.

Topical Co-Delivery of Indomethacin and Nigella Sativa L. Essential Oil in Poly- -Caprolactone Nanoparticles: In Vivo Study of Anti-Inflammatory Activity.

Thymoquinone in liposomes: a study of loading efficiency and biological activity towards breast cancer

Thymoquinone (TQ) regulates cyclooxygenase-2 expression and prostaglandin E2 production through PI3kinase (PI3K)/p38 kinase pathway in human breast cancer cell line, MDA-MB-231

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