Retraction: Products of dentin matrix protein-1 degradation by interleukin-1β-induced matrix metalloproteinase-3 promote proliferation of odontoblastic cells

Hase, Naoko; Ozeki, Nobuaki; Hiyama, Taiki; Yamaguchi, Hideyuki; Kawai, Rie; Kondo, Ayami; Nakata, Kohei; Mogi, Makio

doi:10.5582/bst.2015.01092

Cited by 6 publications

(5 citation statements)

References 42 publications

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“…On the other hand, locally produced IL-1β and TNF-α exert significant influence on odontoblasts functions, inducing further β-defensin production 22 , production of dental matrix protein -1 and inducing proliferation of odontoblast-like cells derived from stem cells. 23 Moreover, the studies investigated the cytokine profile in the GCF samples following dental restoration and reported controversial data 24,25,26,27,28,29,30 The hypothesis of the present study was that IL-1β and TNF-α profile in the GCF from caries affected and intact teeth are different, while the caries extension, tooth position, and different restorative biomaterials alters the biomarker profile as well.…”

Section: Introductionmentioning

confidence: 77%

The effect of dental caries and restorative biomaterials on IL-1 β and TNF-α levels in the gingival crevicular fluid

et al. 2021

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Background/Aim.To estimate the concentrations of the proinflammatory cytokines (IL-1β TNF-in the gingival crevicular fluid (GCF) samples from caries affected teeth and intact teeth, and to correlate them with caries extension, tooth position, and different restorative biomaterials. Methods.GCF samples were collected from the 90 periodontally healthy patients demonstrating at least one tooth with proximal caries and one intact tooth, at the baseline, 7 and 30 days post-treatment. The biomarkers profile was investigated in relation to the different level of caries extension (superficial, pulpitis, gangrenous, root affection), defect size and restorative biomaterial. Results. Before therapy, caries level was significantly associated with GCF IL-1β concentration, demonstrating lowest level in gangrenous (C4) and superficial caries (C2). 30d after therapy, root affection (C5) was characterized by the highest IL-1β concentration. Different dental fillings showed various GCF cytokine change. CPC induced a significant IL-1β increase in more than 70% of treated patients. Caries lesion size was insignificantly associated with GCF levels of these proinflammatory cytokines, where larger defects were followed with average cytokine increase. Considering tooth position, before therapy, IL-1β had the highest level in GCF samples from caries affected canines and second molars while TNF-α showed highest levels from canines GCF. Dental restoration induced cytokine increase in canines (IL-1β and TNF-α), 1st and 2nd molars GCF (IL-1β). Conclusion. Before inflammation intensity of tooth structures was directly reflected with IL-1β and TNF-α concentration. Dental restoration significantly affects IL-1β and TNF-α depending on the used dental filling-type material. Profile of these cytokines varied in GCF samples of the tooth with different anatomical position, where canines and molars demonstrated the highest level. An increase of these proinflammatory cytokines in the absence of any symptomatic manifestation of inflammatory response has to be reconsidered as a possible tooth reparation parametar. Clinical relevance. The concentration of GCF cytokines is illustrative in depicting processes in tooth structures. Their relevance must be inspected with aspects of tooth position and caries lesion level. Different impact of dental restoration materials on GCF IL-1β and TNF-αcould be used as parameter for estimating local inflammation.

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Section: Introductionmentioning

confidence: 77%

The effect of dental caries and restorative biomaterials on IL-1 β and TNF-α levels in the gingival crevicular fluid

et al. 2021

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“…However, this study also showed that there was no significant difference between the viability of hPDLSCs treated with 1 µg/ml and 2 µg/ml. In accordance with the previous studies, DMP1 could induce proliferation in the different cell types (Hase et al, 2015;Maglic et al, 2015;Ye et al, 2005). In addition, the RGD domain of DMP1 was postulated to enhance the proliferation of odontoblast like cells (Hase et al, 2005).…”

Section: Proliferation Assay Of E Coli-produced Hdmp1supporting

confidence: 81%

Expression of human dentin matrix protein 1 in escherichia coli and nicotiana benthamiana and their effect on proliferation and osteogenic differentiation of human periodontal ligament stem cells

Ahmad

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This study aimed to express recombinant protein human Dentin Matrix Protein 1 (hDMP1) in Escherichia coli and Nicotiana benthamiana and investigated the osteogenic differentiation effects in human Periodontal Ligament Stem Cells (hPDLSCs). The gene encoding hDMP1 was ligated into the pET22b and pBY2R2e expression vectors. Recombinant hDMP1 protein has been productively expressed in E. coli and N. benthamiana. E. coli-produced hDMP1 resulted in 60 kDa and 100, and the optimal condition to produce highly expression was demonstrated at 0.5 mM IPTG and incubation at 37oC for 6 hr. N. benthamiana-produced hDMP1 protein showed in a 100 kDa that indicated the presence of post-translational modification, and the highest-level expression was obtained at 2 dpi and OD600 0.4. Recombinant hDMP1 could induce ALP, BMP2, CBFA1, OSX, OPN, and WNT3a osteogenic marker genes and calcium deposition in hPDLSCc. In conclusion, the results indicated that recombinant hDMP1 could induce osteogenic differentiation in hPDLSCs. However, N. benthamiana-produced hDMP1 could induce osteogenic differentiation and calcium deposition better than E. coli-produced hDMP1. Therefore, N. benthamiana-produced hDMP1 has potential to use as an inductive molecule in bone tissue�engineering.

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“…DMP1 was previously shown to induce the proliferation of different cell types [ [21] , [22] , [23] ]. In this study, DMP1 produced from E. coli was tested for toxicity on hPDL cells using the MTT assay.…”

Section: Discussionmentioning

confidence: 99%

Recombinant human dentin matrix protein 1 (DMP1) induces the osteogenic differentiation of human periodontal ligament cells

Ahmad

Kaewpungsup

Khorattanakulchai

et al. 2019

Biotechnology Reports

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Highlights Recombinant human dentin matrix protein 1 (DMP1) can be produced in Escherichia coli . E. coli produced DMP1 could induce the expression of osteogenic-related genes and calcium deposition in human PDL cells. This protein has potential to use for improving tooth repair and regeneration in the future.

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Retraction: Products of dentin matrix protein-1 degradation by interleukin-1β-induced matrix metalloproteinase-3 promote proliferation of odontoblastic cells

Cited by 6 publications

References 42 publications

The effect of dental caries and restorative biomaterials on IL-1 β and TNF-α levels in the gingival crevicular fluid

The effect of dental caries and restorative biomaterials on IL-1 β and TNF-α levels in the gingival crevicular fluid

Expression of human dentin matrix protein 1 in escherichia coli and nicotiana benthamiana and their effect on proliferation and osteogenic differentiation of human periodontal ligament stem cells

Recombinant human dentin matrix protein 1 (DMP1) induces the osteogenic differentiation of human periodontal ligament cells

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