Retrospective study of porcine circovirus 3 infection in China

Sun, Jixiang; Li, Wei; Lu, Zexi; Mi, Shijiang; Bao, Fangyin; Guo, Huancheng; Tu, Changchun; Zhu, Yan; Gong, Wenjie

doi:10.1111/tbed.12853

Cited by 66 publications

(62 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, other studies have confirmed that there is no significant genetic difference in PCV3 strains in different regions. Previous studies have shown that the high genetic stability of pigorigin PCV3 over the past 20 years (Sukmak et al, 2019;Sun et al, 2018). In the present study, the dog-origin PCV3 strain in this study share hight nucleic acid similarity (> 95%) with reference sequences of pig-origin PCV3.…”

Section: Discussionsupporting

confidence: 61%

“…Subsequently, this virus has been detected in many countries and has an abnormally high positive rate (Costa et al, 2014;Deim et al, 2019;Faccini et al, 2017;Saraiva et al, 2019). In China, multiple epidemiological surveillance data indicated that this virus was extensively prevalent in pigs in many provinces and specific cities (Qi et al, 2019;Sun et al, 2018). Multiple studies have revealed that the virus was associated with several diseases (such as PDNS, multisystemic inflammation, reproductive failure) and coinfects with other viruses (for example, Torque teno sus virus, PCV2, porcine reproductive and respiratory syndrome virus) Ha et al, 2018;Wen et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An epidemiological investigation of porcine circovirus 3 infection in dogs in the Guangxi Province from 2015 to 2017, China

et al. 2019

View full text Add to dashboard Cite

A B S T R A C TPorcine circovirus type 3 (PCV3) is an emerging circovirus species associated with several diseases. The study aimed to investigate the frequency of porcine circovirus 3 (PCV3) and its coinfection with canine parvovirus type 2 (CPV-2) in dogs in the Guangxi province from 2015 to 2017, China, and to examine the genome diversity of PCV3. Using polymerase chain reaction (PCR) amplification and sequencing, 96 of 406 (23.6%)samples were positive for PCV3, 38 out of 406 (9.4%) samples were coinfected with both PCV3 and CPV-2. The CPV-positive rate was significantly higher in the PCV3-positive samples than in the non-PCV3 samples, and the difference was extremely significant (P < 0.01). The complete genome (n=4) and ten capsid genes (n=10) of PCV3 were sequenced. Multiple sequence alignment results showed that these sequences shared 98.5-100% nucleotide similarity with the reference genome sequence and 97.5-100% nucleotide similarity with the reference capsid gene sequence. PCV3 was classified into two different genotypes, according to phylogenetic analysis based on the whole genome. These strains were clustered in PCV3a, showing a close relationship with PCV3-US/SD2016. Surprisingly, we separately analyzed these PCV3 strains from the Guangxi province and found that the dog and pig PCV3 are from different branches. In summary, this was the first seroprevalence and genetic investigation of PCV3 in dogs in the Guangxi province, China, and the first complete genome PCV3 from dogs obtained in the world. The results provide insights into the epidemiology and pathogenesis of this important virus.

show abstract

Section: Discussionsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

An epidemiological investigation of porcine circovirus 3 infection in dogs in the Guangxi Province from 2015 to 2017, China

et al. 2019

View full text Add to dashboard Cite

show abstract

“…PCV3 has been reported in major pig-producing countries since the first report in the US in 2016 [6][7][8][9][10][11][12][13][14][15][16][17]. The virus was reported in China in samples taken in 2015 [8] with its possible presence traced back as early as 1996 as revealed in another study [25]. By qPCR, we found that PCV3 was present in two-thirds of the clinical samples including archived specimen back from 2014.…”

Section: Discussionsupporting

confidence: 52%

“…The sequences from Zhejiang province were distributed in all three groups, while most of them were present in the group PCV3c and clustered into a small branch together with the USA isolate PCV3-US/MO2015. Some studies reported the patterns of key amino acid residues 24, 27, 77 and 150 on PCV3 ORF2 [13,24,25]. We analyzed these four residues by extending to the 200 isolates of the three groups ( Fig.…”

Section: Phylogenetic Analysis Of Pcv3 Orf2mentioning

confidence: 99%

Prevalence of porcine circovirus type 3 in pigs in the southeastern Chinese province of Zhejiang

et al. 2019

View full text Add to dashboard Cite

Background Porcine circovirus type 3 (PCV3) was first reported in US in 2016. The virus was also identified later in China. Prevalence of PCV3 in Zhejiang province in southeastern China is not clear though it has been reported in many parts of China. Results PCV3 infection and its co-infection with other swine viral pathogens in pig herds of Zhejiang province were retrospectively investigated by quantitative PCR (qPCR) and its sero-prevalence by indirect ELISA. PCV3 was found positive in 67.1% of the 283 clinical samples taken from 2014 to 2017 as shown by qPCR. Single infection with PCV3 accounted for only one-third of the samples, and majority were of co-infections, predominantly with PEDV (41.6%) but generally low with other swine viruses. Indirect ELISA using the PCV3 capsid protein as the coating antigen revealed an average sero-positive rate of 52.6% (40.8 to 60.8%) in 2345 serum samples from 2011 to 2017, with earliest yet high positive findings in samples taken in 2012. Of 203 serum samples, the qPCR method showed more positive findings than ELISA (81.3% vs 56.2%). With 89 serum samples negative by ELISA, vast majority ( n = 81) were found positive by qPCR. There was negative correlation in levels of PCV3 DNA and anti-capsid antibody response. ORF2-based phylogenetic analysis revealed three major groups (PCV3a, PCV3b and PCV3c) of the 200 strains, 38 from this study and 162 reference strains from GenBank. Most of the strains from this study were clustered into PCV3c. Of the putative signature residues of the capsid protein (aa 24, 27, 77 and 150) relative to the three groups, only the PCV3a group strains showed a distinct pattern of residues VKSI (95% of the strains), while the other two groups did not have such a ‘signature’ pattern. Conclusions Results from this study provided further evidence that the novel virus PCV3 was widely distributed in China and might have emerged in Zhejiang province before 2014, most probably back in 2012 when there was high PCV3 sero-prevalence. PCV3 might be viremic in pigs and could spread by fecal shedding. Electronic supplementary material The online version of this article (10.1186/s12917-019-1977-7) contains supplementary material, which is available to authorized users.

show abstract

“…The emerging PCV3 has been reported in Chinese swine herds since 2017, and the genetic similarity of the isolate with the above‐reported US isolates was strong (Fan et al, ). A retrospective study of PCV3 has demonstrated that the earliest cases may predate to 1996 (Sun et al, ). Several studies have been performed and have shown a wide prevalence of PCV3 in China (Wen et al, ; Zheng et al, ; Ku et al, ).…”

Section: Introductionmentioning

confidence: 99%

The prevalence of novel porcine circovirus type 3 isolates in pig farms in China

Liu

Zhang

Song

et al. 2019

Transbound Emerg Dis

View full text Add to dashboard Cite

The emerging porcine circovirus type 3 (PCV3) has been reported in Chinese swine herds since 2017. We performed a nationwide investigation on the prevalence of PCV3 in pig breeding farms and slaughterhouses in China. A total of 4,040 tonsil samples were collected from 89 farms in 25 provinces, and 1,419 lymph node samples were collected from 50 slaughterhouses in 27 provinces. The PCR results showed that in pig breeding farms, the positive rate was 41.6% (37/89) at the farm level and 5.0% (201/4040) at the individual level. In the slaughterhouses, the positive rate was 62.0% (31/50) at the farm level and 8.0% (114/1419) at the individual level. The PCR‐positive samples were further sequenced, and 19 new PCV3 isolates were identified. The complete genomes of the 19 virus isolates showed 97.4%–99.7% nucleotide identity with other PCV3 isolates. The phylogenetic analysis revealed that the 19 isolates were divided into PCV3a and PCV3b genotype clusters based on the PCV3 complete genome sequences. This study indicated that PCV3 has spread extensively in both pig breeding farms and slaughterhouses. The positive rate of PCV3 was higher in eastern China compared to other regions in China. Furthermore, this study will help us understand the prevalence and genetic variation of PCV3 in Chinese swine herds.

show abstract

Retrospective study of porcine circovirus 3 infection in China

Cited by 66 publications

References 20 publications

An epidemiological investigation of porcine circovirus 3 infection in dogs in the Guangxi Province from 2015 to 2017, China

An epidemiological investigation of porcine circovirus 3 infection in dogs in the Guangxi Province from 2015 to 2017, China

Prevalence of porcine circovirus type 3 in pigs in the southeastern Chinese province of Zhejiang

The prevalence of novel porcine circovirus type 3 isolates in pig farms in China

Contact Info

Product

Resources

About